A practical toxicity bioassay for vicine and convicine levels in faba bean (<scp><i>Vicia faba</i></scp>)

Getachew, Fitsum; Vandenberg, Albert; Smits, Judit E. G.

doi:10.1002/jsfa.9049

Cited by 10 publications

(3 citation statements)

References 26 publications

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“…Those results suggest that vc-faba bean varieties are potentially safe for patients who are lacking G6PD, but further studies with more patients are required [205]. Moreover, new analytical procedures have been developed to quantify vicine and convicine in food products to assure food safety [71,206,207].…”

Section: Favismmentioning

confidence: 99%

Faba Bean: An Untapped Source of Quality Plant Proteins and Bioactives

et al. 2022

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Faba beans are emerging as sustainable quality plant protein sources, with the potential to help meet the growing global demand for more nutritious and healthy foods. The faba bean, in addition to its high protein content and well-balanced amino acid profile, contains bioactive constituents with health-enhancing properties, including bioactive peptides, phenolic compounds, GABA, and L-DOPA. Faba bean peptides released after gastrointestinal digestion have shown antioxidant, antidiabetic, antihypertensive, cholesterol-lowering, and anti-inflammatory effects, indicating a strong potential for this legume crop to be used as a functional food to help face the increasing incidences of non-communicable diseases. This paper provides a comprehensive review of the current body of knowledge on the nutritional and biofunctional qualities of faba beans, with a particular focus on protein-derived bioactive peptides and how they are affected by food processing. It further covers the adverse health effects of faba beans associated with the presence of anti-nutrients and potential allergens, and it outlines research gaps and needs.

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Section: Favismmentioning

confidence: 99%

Faba Bean: An Untapped Source of Quality Plant Proteins and Bioactives

et al. 2022

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“…e percentage of this hemolysis is correlated with the G6PD deficiency levels as noticed in Table 1. Getachew et al previously demonstrated that fava bean aglycones cause an irreversible rapid GSH depletion with a simultaneous increase in oxidized glutathione (GSSG) production in G6PD enzyme defective human RBCs [24]. is was correlated with progressive accumulation of denatured hemoglobin products into high molecular weight (HMW) proteins and the formation of both band 3 membrane proteins and hemichromes as HMW protein aggregates [23,24].…”

Section: Resultsmentioning

confidence: 99%

Ex Vivo Study of Laban's Role in Decreasing Hemolysis Crisis in G6PD-Deficient Patients

Zam

Belal²

2020

Journal of Nutrition and Metabolism

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In spite of the vast nutritional and environmental benefits provided by fava bean (Vicia faba), the ingestion of vicine/convicine provokes an acute hemolytic anemia called favism in individuals with a glucose-6-phosphate dehydrogenase (G6PD) deficiency. The elimination of these glycosides is a goal that could be accomplished using different processing methods including bacteriological treatment. Laban as a good source of lactic acid bacteria was tested in an ex vivo assay on human blood samples in order to determine its capacity in decreasing the hemolysis crisis induced by the ingestion of fava beans. Results indicate a significant decrease in human blood cell hemolysis after the treatment of fava beans by Laban. This decrease in hemolysis was also correlated with the G6PD deficiency categorization. The highest hemolysis level (mean: 23.11 ± 0.76%) was observed in samples with G6PD activity between 10 and 30%, while the lowest hemolysis level (mean: 5.75 ± 0.64%) was observed in samples with G6PD activity more than 60%. This decrease was correlated with a high antioxidant capacity of Laban (51.61 ± 1.13% expressed by the percentage inhibition of DPPH radical). The counts of isolates from MRS and M17 culture plates were 6.75 ± 0.095 and 7.91 ± 0.061 log cfu ml–1, respectively. In conclusion, the synergy between the antioxidant properties of Laban and the possible decrease of vicine and convicine concentrations by lactobacillus found in the fermented dairy products could explain the ability of Laban to reduce the hemolysis crisis ex vivo.

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“…Traditional means of estimating V-C contents are expensive and require highly sophisticated equipment. Recent efforts have led to the successful development of a new biological test (bioassay) and laboratory methods to detect V-C content in faba bean (2). These methods are relatively inexpensive and can have a turn-around time of as little as one week.…”

Section: The Importance Of V-cmentioning

confidence: 99%

New Opportunities for Faba Bean

2018

CFW

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A practical toxicity bioassay for vicine and convicine levels in faba bean (Vicia faba)

Abstract: BCNU-sensitized red blood cells provide an ideal model for favism blood, to assess and compare the toxicity of faba bean varieties and their food products. © 2018 Society of Chemical Industry.

Cited by 10 publications

References 26 publications

Faba Bean: An Untapped Source of Quality Plant Proteins and Bioactives

Faba Bean: An Untapped Source of Quality Plant Proteins and Bioactives

Ex Vivo Study of Laban's Role in Decreasing Hemolysis Crisis in G6PD-Deficient Patients

New Opportunities for Faba Bean

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