A practical way to prepare primer human chondrocyte culture

Isyar, Mehmet; Yılmaz, İbrahim; Şirin, Duygu Yaşar; Yalçın, Sercan; Güler, Olcay; Mahiroğulları, Mahir

doi:10.1016/j.jor.2016.03.008

Cited by 19 publications

(44 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As it is known that the sensitivity of human and animal tissues is different, therefore, the results obtained can be different and misleading as well . In many studies where animal tissues are not used, commercial cell lines are used …”

Section: Discussionmentioning

confidence: 99%

“…As a result, the in vitro test results, which are difficult to comply with in vivo conditions, may become even more controversial since the cell does not interact with formations such as extracellular matrix structure. Besides, since the genetic structure of the cells in the cell line has been modified, they will not carry the genotypic and/or phenotypic characteristics that they have in the human body …”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Are radio‐contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

Karaarslan

Yılmaz

Özbek

et al. 2018

Basic Clin Pharma Tox

Self Cite

View full text Add to dashboard Cite

In the literature, there have been no studies showing clear results on how radio-contrast pharmaceuticals would affect intact disc tissue cells. In this context, it was aimed to evaluate the effects of iopromide and gadoxetic acid, frequently used in the discography, on intact lumbar disc tissue in pharmaco-molecular and histopathological level. Primary cell cultures were prepared from the healthy disc tissue of the patients operated in the neurosurgery clinic. Except for the control group, the cultures were incubated with the indicated radio-contrast agents. Cell viability, toxicity and proliferation indices were tested at specific time intervals. The cell viability was quantitatively analysed. It was also visually rechecked under a fluorescence microscope with acridine orange/propidium iodide staining. Simultaneously, cell surface morphology was analysed with an inverted light microscope, while haematoxylin and eosin (H&E) staining methodology was used in the histopathological evaluations. The obtained data were evaluated statistically. Unlike the literature, iopromide or gadoxetic acid did not have any adverse effects on the cell viability, proliferation and toxicity (P < 0.05). Although this study reveals that radio-contrast pharmaceuticals used in the discography, often used in neurosurgical practice, can be safely used, it should be remembered that this study was performed in an in vitro environment.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Are radio‐contrast agents commonly used in discography toxic to the intact intervertebral disc tissue cells?

Karaarslan

Yılmaz

Özbek

et al. 2018

Basic Clin Pharma Tox

Self Cite

View full text Add to dashboard Cite

show abstract

“…MTT analyses were evaluated in accordance with commercial kit bulletins on the 1st, 7th, 14th, and 21st days [15–20, 24, 26]. …”

Section: Methodsmentioning

confidence: 99%

Can a Biodegradable Implanted Bilayered Drug Delivery System Loaded with BMP-2/BMP-12 Take an Effective Role in the Biological Repair Process of Bone–Tendon Injuries? A Preliminary Report

Kömür

Akyuva

Karaslan

et al. 2017

Journal of Pharmaceutics

Self Cite

View full text Add to dashboard Cite

Background Use of biodegradable and biocompatible materials in the orthopedic surgery is gaining popularity. In this research, the rate of controlled release of a bilayered prototype biomaterial designed to promote osteoblastic and tenoblastic activity was calculated using pharmacochemical methods. Methods The first part of the design, composed of a sodium tetraborate, polyvinyl alcohol, and starch based hydrogel, was loaded with bone morphogenic protein-2. The second part which was composed of a sodium tetraborate, polyvinyl alcohol, and chitosan based hydrogel was loaded with bone morphogenic protein-12. Osteochondral and tendon tissue specimens were obtained from patients with a diagnosis of gonarthrosis and primary bone cells and tendon cells cultures were prepared following treatment with collagenase enzyme. Cell samples were collected from the groups by means of an invert light microscope and environmental scanning electron microscope underwent at the 1st and 21st days. The level of osteogenic differentiation was measured by the activity of alkaline phosphatase. For the statistical evaluation of the obtained data, groups were compared with post hoc Tukey test following analysis of variance. Level of significance was accepted to be <0,01. Results Both osteogenic and tenogenic stimulation were observed in the cultured specimens. In comparison to the control groups, the rate of proliferation of healthy cells was found to be higher in the groups to which the design was added (p < 0.01). Conclusions Our research is a preliminary report that describes a study conducted in an in vitro experimental setting. We believe that such prototype systems may be pioneers in targeted drug therapies after reconstructional surgeries.

show abstract

“…Recent well-proved studies have reported that half-life of growth factors is maximum 2 min. [37][38][39][40] It was emphasized that when growth factors -with protein and peptide structure -in PRP obtained from human were applied after that period, bioactivity decreased and even vanished. [37][38][39][40] When the studies in which PRP was obtained from autologous blood in clinics were considered, it was found that it was prepared from 10 to 20 min.…”

Section: -2234mentioning

confidence: 99%

“…[37][38][39][40] It was emphasized that when growth factors -with protein and peptide structure -in PRP obtained from human were applied after that period, bioactivity decreased and even vanished. [37][38][39][40] When the studies in which PRP was obtained from autologous blood in clinics were considered, it was found that it was prepared from 10 to 20 min. [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] Nevertheless, it takes time to apply it to cases, which makes its bioactive and effective treatment results controversy.…”

Section: -2234mentioning

confidence: 99%