A predictive model for blastocyst formation based on morphokinetic parameters in time-lapse monitoring of embryo development

Milewski, Robert; Kuć, Paweł; Kuczyńska, Agnieszka; Stankiewicz, Bożena; Łukaszuk, Krzysztof; Kuczyński, Waldemar

doi:10.1007/s10815-015-0440-3

Cited by 99 publications

(86 citation statements)

References 16 publications

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“…The identification of the best quality embryos with the highest blastocyst formation potential could allow the uter- ine transfer of embryos in the second or third day of their life, before they achieve the blastocyst stage [7]. Elimination of the prolonged in vitro culturing may be beneficial, e.g.…”

Section: Discussionmentioning

confidence: 99%

“…All absolute morphokinetic parameters and a majority of relative parameters are non-monotonic since very low to the highest reproductive potential will be close to the median value in the "positive outcome" group of embryos [7,8]. Therefore, the median value in the groups of embryos developed to the blastocyst stage, a biochemical and a clinical pregnancy was estimated (Tab.…”

Section: Methodsmentioning

confidence: 99%

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Morphokinetic parameters as a source of information concerning embryo developmental and implantation potential

et al. 2016

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Objectives: The aim of the study was to present the results of time-lapse observation and to verify whether morphokinetic parameters are associated with embryo developmental and implantation potential. Material and methods:The analysed data concern the development of 1,060 embryos, 898 of which (84.72%) achieved the blastocyst stage and 307 were transferred into the uterine cavity. As a result, 126 (41.04%) biochemical pregnancies and 109 (35.50%) clinical pregnancies were observed. Time from fertilisation to further divisions into 2-9 blastomeres, first to fourth round of cleavage, second to third synchronisation parameters and the duration of stages after the first, second and third division were analysed. Results:Most of the parameters in the group of embryos developed to the blastocyst stage reached lower values than in the non-developed group. Moreover, parameters in the first group clearly had less dispersion. The differences between the groups with and without a biochemical pregnancy were smaller than the differences in the analysis of development to the blastocyst stage. However, in the case of clinical pregnancy analysis, there were again larger differences between both groups. A strong correlation was found between the majority of absolute morphokinetic parameters. A weaker, but still statistically significant correlation, was established between relative and other parameters. Conclusions:Morphokinetic parameters are associated with embryo developmental and implantation potential and can be considered as predictors of their quality. However, the development of efficient pregnancy prediction models needs further research utilising information from all available parameters and using advanced biostatistical methods.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Morphokinetic parameters as a source of information concerning embryo developmental and implantation potential

et al. 2016

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“…Milewski et al create two predictive models for blastocyst development [41] and the transferred embryo implantation ability [42]. They evaluated the embryo morphokinetic parameters between embryos developed to blastocyst, and embryos did not reach blastocyst stage [41] as well as between implanted and non-implanted embryos [42].…”

Section: Morphokinetic Algorithms For Prediction Of Embryo Implantatimentioning

confidence: 99%

“…They evaluated the embryo morphokinetic parameters between embryos developed to blastocyst, and embryos did not reach blastocyst stage [41] as well as between implanted and non-implanted embryos [42]. Based on the indings and using statistical analysis, two models were generated which presented TLS as a good predictive instruments for embryo implantation but not as high as the model for blastocyst for-mation.…”

Section: Morphokinetic Algorithms For Prediction Of Embryo Implantatimentioning

confidence: 99%

Embryo Morphokinetics Based on Time-Lapse Observation

Tabibnejad¹

2017

Embryo Cleavage

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Embryo incubation and evaluation are critical steps in assisted reproductive technology (ART). Conventionally, embryo assessment has been done by embryologists through removing embryos from a conventional incubator during the culture period. Over recent years, time-lapse systems (TLS) have been established which can take digital images of embryos at key points and time intervals. This technique allows embryologists to assess the embryo quality in the steady culture environment. According to TLS studies and prepared algorithm models, it seems that TLS alone or in combination with conventional morphology can be considered as a useful diagnostic tool to determine high-quality embryos and improve embryonic implantation and pregnancy rates. In addition, there were remarkable diferences between embryo developmental time points and intervals regarding embryo gender, embryo fragmentation, and type of ovarian stimulation protocol. For conident conclusion, time-lapse imaging should be evaluated in further studies, and the system should be evaluated for cost/beneit ratio efectiveness in individual laboratory.Keywords: embryo cleavage, embryo morphokinetics, embryoscope, time-lapse imaging IntroductionAssisted reproductive technology (ART) may help infertile couples to realize their dream to have a child in their family, but pregnancy and live birth rates following in vitro fertilization (IVF) still remain low. It is ideal to identify viable embryos with the highest implantation potential to raise IVF success rates. In the traditional IVF practice, embryo assessments are mainly based on the morphologic observation and grade of embryologists at each stage of oocyte and embryonic development. Some features including oocyte and embryo quality, blastomere numbers and regularity, the percentage of fragmentation, and cytoplasmic granularity have been deined as prognostic indicators of successful pregnancy. This traditional embryo assessment method may have some detrimental efects on embryo growth because frequently opening and closing of incubators often cause to the change of embryo culture environmental steadiness. In order to reduce the inter-and intra-observer diference change, the time-lapse imaging (TLI) has been introduced into in vitro fertilization (IVF) laboratory. The application of time-lapse technology to the clinical IVF laboratory has supported more detailed observations on the embryo development researches quickly.The aim of this chapter is to determine whether TLI is useful for selection of "top quality" embryos for transfer to improve ART outcome rather than conventional morphological evaluation. The possible correlation between embryos' sex, embryo fragmentation, treatment protocols, diferent culture media, and embryo morphokinetics will be examined based on some new researches of TLI facilities. Furthermore, various algorithms and predictive models designed in ART cycles with TLI will be discussed.

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“…The timing of key parameters from time-lapse monitoring for predictions of blastocyst formation [11][12][13] aneuploidy, [14,15], and, in some cases, implantation [16][17][18] have been investigated. Various models based on specific developmental milestones and phenotypes have been built [9].…”

Section: Available Time-lapse Systems and Potential Benefitsmentioning

confidence: 99%

A critical appraisal of time-lapse imaging for embryo selection: where are we and where do we need to go?

Racowsky

Kovács

Martins

2015

J Assist Reprod Genet

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Purpose The aim of this study was to undertake a critical appraisal of the available evidence for the use of time-lapse imaging for embryo selection in clinical IVF. Methods A literature search in PubMed, Scopus, Cochrane Central, ClinicalTrials.gov, Current Controlled Trials, and WHO International Clinical Trials Registry Platform was performed to identify randomized controlled trials that investigated the effect of time-lapse embryo selection and/or the timelapse incubation system on ongoing pregnancy rate. We then performed a systematic review and assessed the relative risks (RRs) and 95 % confidence intervals (CIs) for ongoing pregnancy rates and the risk of bias of the eligible studies. Results We identified four eligible randomized studies, three of which investigated the effect of both time-lapse incubation system and selection on ongoing pregnancy rate; the pooled result revealed a benefit of this intervention (relative risk (RR) 1.20; 95 % CI 1.05-1.37). However, the evidence was judged to be of low quality due to study limitations; a beneficial effect was observed in only one study deemed to be at high risk of bias. The single study assessing the effect of only the timelapse incubation system revealed a non-significant negative effect (RR 0.71; 95 % CI 0.49-1.03). Conclusions The findings from this systematic review of the current evidence do not support routine use of time-lapse technology in clinical IVF. We therefore believe that the use of time-lapse imaging for embryo selection should remain experimental and that couples should not be subject to a surcharge for having their embryos cultured in a time-lapse imaging system. Future studies evaluating this technology in welldesigned trials should be performed.

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A predictive model for blastocyst formation based on morphokinetic parameters in time-lapse monitoring of embryo development

Cited by 99 publications

References 16 publications

Morphokinetic parameters as a source of information concerning embryo developmental and implantation potential

Morphokinetic parameters as a source of information concerning embryo developmental and implantation potential

Embryo Morphokinetics Based on Time-Lapse Observation

A critical appraisal of time-lapse imaging for embryo selection: where are we and where do we need to go?

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