A preliminary DNA barcode selection for the genus Russula (Russulales, Basidiomycota)

Abstract

Russula is a worldwid genus which has a high species diversity . Aiming accurate and rapid species identification, candidate genes nLSU (28S), ITS, tef-1α, mtSSU, rpb1, and rpb2, were analysed as potential DNA barcodes. This analysis included 433 sequences from 38 well-circumscribed Russula species of eight subgenera. Two vital standards were analysed for success species identification using DNA barcodes, specifically inter-and intra-specific variations together with the success rates of PCR amplification and sequencing. Although the gap between inter-and intra-specific variations was narrow, ITS met the qualification standards for a target DNA barcode. Overlapping inter-and intra-specific pairwise distances were observed in nLSU, tef-1α, mtSSU, and rpb2. The success rates of PCR amplification and sequencing in mtSSU and rpb1 were lower than those of others. Gene combinations were also investigated for resolution of species recognition. ITS-rpb2 was suggested as the likely target DNA barcode for Russula, owing to the two viatal standards above. Since nLSU has the lowest minimum of inter-specific variation, and tef-1α has the highest overlap between intra-and inter-species variations among the candidate genes, they are disqualified from the selection for DNA barcode of Russula. ARTICLE HISTORY

Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa

“…This genus is characterized by fairly large basidiocarps, brightly colored pileus, white to dark yellow basidiospore print, attached lamellae, absence of volva and milky latex, amyloid basidiospore ornamentations, and numerous spherocysts in context (Sarnari 1998). Over 800 species are accommodated in this genus Li et al 2015Li et al , 2018c. Most recent new Russula species were described based on specimens from Asia (Li et al 2016(Li et al , 2018aSang et al 2016;Das et al 2017aDas et al , b, 2018bHyde et al 2016Hyde et al , 2017bJiang et al 2017;Zhang et al 2017).…”

Antioxidant Extracts of Three Russula Genus Species Express Diverse Biological Activity

“…The genus Russula Pers. belonging to the Russulaceae family comprises more than 780 species with a cosmopolitan distribution, making it the second largest genus in the Agaricomycetes class [ 15 ]. Most of the species have not been thoroughly studied for their bioactive compounds profile and diverse biological activities.…”

Three new Russula species in sect. Ingratae (Russulales, Basidiomycota) from southern China

“…The modern taxonomy of Russula calls for a combination of detailed microscopic observations with universal and specific standard, multi-gene phylogenetic analyses and accurate symbiotic plant species information (Buyck et al 2018;Adamčík et al 2019). The ITS phylogenetic analyses are the most common for practical identification of Russula species, because ITS is regarded as an adequate single gene DNA barcode for this genus (Li et al 2019) and it has the largest number of available referential sequences in open databases (Schoch et al 2012). A combination of morphological and ITS phylogenetic analyses supported the three new species amongst Asian Ingratae: R. straminella, R. subpectinatoides and R. succinea.…”