A preliminary molecular epidemiologic study using analysis of variable number of tandem repeats of Acinetobacter baumannii OXA-23 producing strains isolated from hospitals in Rio de Janeiro State, Brazil

Carramachi, Isabel Nogueira; Carvalho, Karyne Rangel; Cruz, Jéssica Gonçalves Vieira da; Zahner, Viviane

doi:10.5123/s2176-62232014000200008

Cited by 1 publication

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, 91% (71/78) of the isolates were positive for bla OXA-23-like amplifi cation. A study conducted by Carvalho et al (17) in 10 public and private hospitals in the City of Rio de Janeiro from 2005 to 2007 showed similar results, with the detection of bla OXA-23-like in 96% of the isolates. This rate is higher than the rates observed in studies conducted in other countries, both nationwide, such as in Taiwan (21.1%), or restricted to a few local hospitals, such as in Romania (84.6%), Tunisia (82%), Greece (72.4%), and Italy (40%) (33) .…”

Section: Discussionmentioning

confidence: 56%

“…Thus, phenotypically identifi ed isolates that possess the bla OXA-51-like gene were considered members of the complex in the present study. Conversely, recent studies have reported the plasmid-encoded carbapenemase OXA-51 in clinical isolates of Acinetobacter nosocomialis and in other genospecies not corresponding to A. baumannii (17) (33) (34) (35) .…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting

Kobs¹,

Ferreira

Bobrowicz

et al. 2016

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Members of the Acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. Oxacillinases are primarily responsible for resistance to carbapenem antibiotics. Higher rates of carbapenem hydrolysis might be ascribed to insertion sequences, such as the ISAba1 sequence, near bla OXA genes. The present study examined the occurrence of the genetic elements bla OXA and ISAba1 and their relationship with susceptibility to carbapenems in clinical isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. Methods: Isolates identifi ed over 6 consecutive years in a general hospital in Joinville, Southern Brazil, were evaluated. The investigation of 5 families of genes encoding oxacillinases and the ISAba1 sequence location relative to bla OXA genes was conducted using polymerase chain reaction. Results: All isolates presented the bla OXA-51-like gene (n = 78), and 91% tested positive for the bla OXA-23-like gene (n = 71). The presence of ISAba1 was exclusively detected in isolates carrying the bla OXA-23-like gene. All isolates in which ISAba1 was found upstream of the bla OXA-23-like gene (n = 69) showed resistance to carbapenems, whereas the only isolate in which ISAba1 was not located near the bla OXA-23-like gene was susceptible to carbapenems. The ISAba1 sequence position of another bla OXA-23-like -positive isolate was inconclusive. The isolates exclusively carrying the bla OXA-51-like gene (n = 7) showed susceptibility to carbapenems. Conclusions: The presence of the ISAba1 sequence upstream of the bla OXA-23-like gene was strongly associated with carbapenem resistance in isolates of the A. calcoaceticus-A. baumannii complex in the hospital center studied.

show abstract

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 97%