A Preliminary Study in the Effect of &amp;lt;i&amp;gt;Astragali radix&amp;lt;/i&amp;gt;, a Qi-Invigorating Herb, on Mitochondria: Insights at Cellular Level and Mouse Tissues Level

Chen, Yu; Li, Mengmei; Cai, Zhongzhen; Chen, Yuming; Wang, Lin; Teng, Jie; Feng, Qian; Li, Caixia; Rein, Glen; Tang, Bruce Qing; Bai, Xuemei

doi:10.4236/cm.2020.113007

Cited by 48 publications

(1 citation statement)

References 20 publications

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“…17 All these findings suggest that natural food derived-NPs have a favorable safety profile, tissue-specific targeting capabilities, and the ability to be produced in large quantities, making them more appealing for clinical applications than their synthetic counterparts. 18–20…”

Section: Introductionmentioning

confidence: 99%

Oral exosome-like nanovesicles from Phellinus linteus suppress metastatic hepatocellular carcinoma by reactive oxygen species generation and microbiota rebalancing

Zu,

Liu,

Chen

et al. 2024

Nanoscale

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Section: Introductionmentioning

confidence: 99%

Oral exosome-like nanovesicles from Phellinus linteus suppress metastatic hepatocellular carcinoma by reactive oxygen species generation and microbiota rebalancing

Zu,

Liu,

Chen

et al. 2024

Nanoscale

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Exosomal Bupivacaine: Integrating Nerve Barrier Penetration Capability and Sustained Drug Release for Enhanced Potency in Peripheral Nerve Block and Reduced Toxicity

Cai,

Li,

Wesselmann

et al. 2024

Adv Funct Materials

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Peripherally injected local anesthetics exhibit limited ability to penetrate peripheral nerve barriers, which limits their effectiveness in peripheral nerve block and increases the risk of adverse effects. In this work, it is demonstrated that exosomes derived from Human Embryo Kidney 293 cells can effectively traverse the perineurium, which is the rate‐limiting barrier within PNBs that local anesthetics need to cross before acting on axons. Based on this finding, these exosomes are used as a carrier for bupivacaine (BUP), a local anesthetic commonly used in clinical settings. The in vitro assessments revealed that the prepared exosomal bupivacaine (BUP@EXO) achieves a BUP loading capacity of up to 82.33% and sustained release of BUP for over 30 days. In rats, a single peripheral injection of BUP@EXO, containing 0.75 mg of BUP, which is ineffective for BUP alone, induced a 2‐h sensory nerve blockade without significant motor impairments. Increasing the BUP dose in BUP@EXO to 2.5 mg, a highly toxic dose for BUP alone, extended the sensory nerve blockade to 12 h without causing systemic cardiotoxicity and local neurotoxicity and myotoxicity.

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High‐Yield Production of Extracellular Vesicle Subpopulations with Constant Quality Using Batch‐Refeed Cultures

Paganini

Boyce

Libort

et al. 2022

Adv Healthcare Materials

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The conventional manufacturing of extracellular vesicles (EVs) is characterized by low yields and batch‐to‐batch variability, hampering fundamental research on EVs and their practical applications. Perfusion operations have huge potential to address these limitations and increase the productivity and quality of EVs. In this study, perfusion cultures are simulated with batch‐refeed systems and their productivity is compared with that achieved using batch cultures. It is shown that a shift from batch to batch‐refeed system can increase the space‐time yields of a target EV subpopulation characterized by CD81 and CD63 biomarkers by threefold. Moreover, it is demonstrated that the method facilitates the consistent production of the target EVs from cells maintained under constant conditions for 13 days. These results indicate that the use of perfusion cultures is a promising strategy to increase the manufacturing yield of EVs and control the production of specific EV subpopulations with constant quality attributes, thereby improving reproducibility.

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A Preliminary Study in the Effect of <i>Astragali radix</i>, a Qi-Invigorating Herb, on Mitochondria: Insights at Cellular Level and Mouse Tissues Level

Cited by 48 publications

References 20 publications

Oral exosome-like nanovesicles from Phellinus linteus suppress metastatic hepatocellular carcinoma by reactive oxygen species generation and microbiota rebalancing

Oral exosome-like nanovesicles from Phellinus linteus suppress metastatic hepatocellular carcinoma by reactive oxygen species generation and microbiota rebalancing

Exosomal Bupivacaine: Integrating Nerve Barrier Penetration Capability and Sustained Drug Release for Enhanced Potency in Peripheral Nerve Block and Reduced Toxicity

High‐Yield Production of Extracellular Vesicle Subpopulations with Constant Quality Using Batch‐Refeed Cultures

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