A Preliminary Study to Assess Possible Chromosomal Damage Among Users of Digital Mobile Phones

Gadhia, Pankaj; Shah, Tejal M.; Mistry, Amit; Pithawala, Meonis; Tamakuwala, Dipesh

doi:10.1081/jbc-120024624

Cited by 25 publications

(11 citation statements)

References 21 publications

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“…Several studies (Juutilainen et al 2010;Hintzsche and Stopper 2010) came to the conclusion that nonionizing radiation exposure could not be related to any genotoxic action, although several studies have reported effects with different genotoxic end points such as sister chromatid exchange (Gadhia et al 2003), micronuclei (Gandhi and Singh 2005a;Gandhi and Anita 2005b;Yadav OR odds ratio, CI confidence interval v 2 test was used for comparison of GSTM1 and GSTT1 genotype distribution among the control and exposed groups Arch Environ Contam Toxicol * Significant at p \ 0.05. Multivariate analysis of covariance tests was used for the differences in tail moment value (TM) and BMN frequency adjusted with age, sex, consumption habits and exposure duration among exposed and control groups separately Arch Environ Contam Toxicol and Sharma 2008), CAs (Lalic et al 2001;Othman et al 2003), and DNA strand breakage or comet assay (Sun et al 2006a, b, c;Garaj-Vrhovac and Orescanin 2009).…”

Section: Discussionmentioning

confidence: 98%

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Effect of GSTM1 and GSTT1 Polymorphisms on Genetic Damage in Humans Populations Exposed to Radiation From Mobile Towers

Gulati

Yadav

Kumar

et al. 2015

Arch Environ Contam Toxicol

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All over the world, people have been debating about associated health risks due to radiation from mobile phones and mobile towers. The carcinogenicity of this nonionizing radiation has been the greatest health concern associated with mobile towers exposure until recently. The objective of our study was to evaluate the genetic damage caused by radiation from mobile towers and to find an association between genetic polymorphism of GSTM1 and GSTT1 genes and DNA damage. In our study, 116 persons exposed to radiation from mobile towers and 106 control subjects were genotyped for polymorphisms in the GSTM1 and GSTT1 genes by multiplex polymerase chain reaction method. DNA damage in peripheral blood lymphocytes was determined using alkaline comet assay in terms of tail moment (TM) value and micronucleus assay in buccal cells (BMN). There was a significant increase in BMN frequency and TM value in exposed subjects (3.65 ± 2.44 and 6.63 ± 2.32) compared with control subjects (1.23 ± 0.97 and 0.26 ± 0.27). However, there was no association of GSTM1 and GSTT1 polymorphisms with the level of DNA damage in both exposed and control groups.

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Section: Discussionmentioning

confidence: 98%

“…The potential genotoxicity of RF-EMR has been assessed in human epidemiological studies that have shown both positive (Gadhia et al 2003;Markova et al 2005;Gandhi and Anita 2005b) and negative reports (Garson et al 1991;Maes et al 2006). Therefore, there is a need to evaluate different exposed populations with a variety of genotoxic parameters.…”

mentioning

confidence: 96%

Effect of GSTM1 and GSTT1 Polymorphisms on Genetic Damage in Humans Populations Exposed to Radiation From Mobile Towers

Gulati

Yadav

Kumar

et al. 2015

Arch Environ Contam Toxicol

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“…There is growing evidence for nonthermal effects of MWs that suggests possible relationship of MW exposure with adverse health effects [Navarro et al, 2003;Santini et al, 2003;Hallberg and Johansson, 2004], immune response in human lymphocytes [Dabrowski et al, 2003], permeability of BBB in rat brains [Persson et al, 1997], increase in calcium ion efflux and ornithine decarboxylase (ODC) activity in brains of exposed rats [Paulraj and Behari, 2002], release of manganese and zinc ions in the serum of the exposed rats [Aksen et al, 2004], decreases in the reproductive capacity of Drosophila melanogaster [Panagopoulos et al, 2004], formation of dicentric chromosomes among mobile users [Gadhia et al, 2003], gene expression in human skin fibroblasts [Pacini et al, 2002], and stress response in human epithelial amnion (AMA) cells [Kwee et al, 2001]. Recent data provided evidence for neuronal damage in both the cortex, the hippocampus and the basal ganglia in the brains of exposed rats [Salford et al, 2003;Ilhan et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

Exposure of rat brain to 915 MHz GSM microwaves induces changes in gene expression but not double stranded DNA breaks or effects on chromatin conformation

Belyaev

Koch

Terenius

et al. 2006

Bioelectromagnetics

113

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We investigated whether exposure of rat brain to microwaves (MWs) of global system for mobile communication (GSM) induces DNA breaks, changes in chromatin conformation and in gene expression. An exposure installation was used based on a test mobile phone employing a GSM signal at 915 MHz, all standard modulations included, output power level in pulses 2 W, specific absorption rate (SAR) 0.4 mW/g. Rats were exposed or sham exposed to MWs during 2 h. After exposure, cell suspensions were prepared from brain samples, as well as from spleen and thymus. For analysis of gene expression patterns, total RNA was extracted from cerebellum. Changes in chromatin conformation, which are indicative of stress response and genotoxic effects, were measured by the method of anomalous viscosity time dependencies (AVTD). DNA double strand breaks (DSBs) were analyzed by pulsed-field gel electrophoresis (PFGE). Effects of MW exposure were observed on neither conformation of chromatin nor DNA DSBs. Gene expression profiles were obtained by Affymetrix U34 GeneChips representing 8800 rat genes and analyzed with the Affymetrix Microarray Suite (MAS) 5.0 software. In cerebellum from all exposed animals, 11 genes were upregulated in a range of 1.34-2.74 fold and one gene was downregulated 0.48-fold (P < .0025). The induced genes encode proteins with diverse functions including neurotransmitter regulation, blood-brain barrier (BBB), and melatonin production. The data shows that GSM MWs at 915 MHz did not induce PFGE-detectable DNA double stranded breaks or changes in chromatin conformation, but affected expression of genes in rat brain cells.

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“…Previous studies observed significant increases in dicentric chromosomes (among smoker-alcoholic mobile users), sister chromatid exchanges (synergistic action with mitomycin C) [6], micro-nucleation and DNA strand breaks [7] in blood lymphocytes obtained from mobile phone users. A significant increase in erythrocyte micronuclei frequency was observed in newborn pups of mother rats maintained under 834 MHz EMF exposure at a SAR of 1.23 W/kg for 8.5 h per day throughout the gestation period [8].…”

Section: Introductionmentioning

confidence: 99%

8-oxoG DNA Glycosylase-1 Inhibition Sensitizes Neuro-2a Cells to Oxidative DNA Base Damage Induced by 900 MHz Radiofrequency Electromagnetic Radiation

Wang

Liu

et al. 2015

Cell Physiol Biochem

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Background/Aims: The purpose of this study was to explore the in vitro putative genotoxicity during exposure of Neuro-2a cells to radiofrequency electromagnetic fields (RF-EMFs) with or without silencing of 8-oxoG DNA glycosylase-1 (OGG1). Methods: Neuro-2a cells treated with or without OGG1 siRNA were exposed to 900 MHz Global System for Mobile Communication (GSM) Talk signals continuously at a specific absorption rate (SAR) of 0, 0.5, 1 or 2 W/kg for 24 h. DNA strand breakage and DNA base damage were measured by the alkaline comet assay and a modified comet assay using formamidopyrimidine DNA glycosylase (FPG), respectively. Reactive oxygen species (ROS) levels and cell viability were monitored using the non-fluorescent probe 2, 7-dichlorofluorescein diacetate (DCFH-DA) and CCK-8 assay. Results: Exposure to 900 MHz RF-EMFs with insufficient energy could induce oxidative DNA base damage in Neuro-2a cells. These increases were concomitant with similar increases in the generation of reactive oxygen species (ROS). Without OGG1 siRNA, 2 W/kg RF-EMFs induced oxidative DNA base damage in Neuro-2a cells. Interestingly, with OGG1 siRNA, RF-EMFs could cause DNA base damage in Neuro-2a cells as low as 1 W/kg. However, neither DNA strand breakage nor altered cell viability was observed. Conclusion: Even if further studies remain conducted we support the hypothesis that OGG1 is involved in the process of DNA base repair and may play a pivotal role in protecting DNA bases from RF-EMF induced oxidative damage.

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A Preliminary Study to Assess Possible Chromosomal Damage Among Users of Digital Mobile Phones

Cited by 25 publications

References 21 publications

Effect of GSTM1 and GSTT1 Polymorphisms on Genetic Damage in Humans Populations Exposed to Radiation From Mobile Towers

Effect of GSTM1 and GSTT1 Polymorphisms on Genetic Damage in Humans Populations Exposed to Radiation From Mobile Towers

Exposure of rat brain to 915 MHz GSM microwaves induces changes in gene expression but not double stranded DNA breaks or effects on chromatin conformation

8-oxoG DNA Glycosylase-1 Inhibition Sensitizes Neuro-2a Cells to Oxidative DNA Base Damage Induced by 900 MHz Radiofrequency Electromagnetic Radiation

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