A prevalent and culturable microbiota links ecological balance to clinical stability of the human lung after transplantation

Das, Sudip; Bernasconi, Eric; Koutsokera, Angela; Wurlod, Daniel-Adrien; Tripathi, Vishwachi; Bonilla-Rosso, Germán; Aubert, John‐David; Derkenne, Marie-France; Mercier, Louis; Pattaroni, Céline; Rapin, Alexis; Garnier, Christophe von; Marsland, Benjamin J.; Engel, Philipp; Nicod, Laurent

doi:10.1038/s41467-021-22344-4

Cited by 42 publications

(59 citation statements)

References 76 publications

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“…Recently, a potential link between the microbial community composition and lung homeostasis was examined by analyzing bronchoalveolar lavage samples from a longitudinally followed post-transplant study population ( Das et al, 2021 ). Although the lung microbiota proved to be highly variable, there were a few bacterial taxa with high prevalence and/or abundance, and the majority of them were either obligate or facultative anaerobes.…”

Section: Prevotella In Bacterial Communities Of the Respiratory Tractmentioning

confidence: 99%

“…Among the most prevalent species were P. melaninogenica , Veillonella atypica , Veillonella dispar , Streptococcus mitis , and Granulicatella adiacens (all typical recoveries from the oral cavity). The microbiota was categorized into four distinct compositional states (pneumotypes) where the balanced pneumotype represented a diverse bacterial community, resembling that in the oropharynx and including P. melaninogenica , which occurred in 97.4% of the samples ( Das et al, 2021 ). In addition, this pneumotype had a high immune-modulatory activity and preserved lung stability.…”

Section: Prevotella In Bacterial Communities Of the Respiratory Tractmentioning

confidence: 99%

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Oral Prevotella Species and Their Connection to Events of Clinical Relevance in Gastrointestinal and Respiratory Tracts

Könönen

Gürsoy

2022

Front. Microbiol.

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Prevotella is recognized as one of the core anaerobic genera in the oral microbiome. In addition, members of this genus belong to microbial communities of the gastrointestinal and respiratory tracts. Several novel Prevotella species, most of them of oral origin, have been described, but limited knowledge is still available of their clinical relevance. Prevotella melaninogenica is among the anaerobic commensals on oral mucosae from early months of life onward, and other early colonizing Prevotella species in the oral cavity include Prevotella nigrescens and Prevotella pallens. Oral Prevotella species get constant access to the gastrointestinal tract via saliva swallowing and to lower airways via microaspiration. At these extra-oral sites, they play a role as commensals but also as potentially harmful agents on mucosal surfaces. The aim of this narrative review is to give an updated overview on the involvement of oral Prevotella species in gastrointestinal and respiratory health and disease.

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Section: Prevotella In Bacterial Communities Of the Respiratory Tractmentioning

confidence: 99%

Section: Prevotella In Bacterial Communities Of the Respiratory Tractmentioning

confidence: 99%

Oral Prevotella Species and Their Connection to Events of Clinical Relevance in Gastrointestinal and Respiratory Tracts

Könönen

Gürsoy

2022

Front. Microbiol.

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“…This could also explain the clustering between pathogenic viruses and GTVs. While GTVs were found frequently in nasopharyngeal secretions of children with acute respiratory disease and also in the context of lung transplantation [ 49 ], it is supposed that they are innocent bystanders more than pathogenic agents [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Metagenomic sequencing reveals time, host, and body compartment-specific viral dynamics after lung transplantation

et al. 2022

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Background The virome of lung transplant recipients (LTRs) under immunosuppressive therapy is dominated by non-pathogenic Anelloviridae and further includes several pathogenic viruses such as Herpesviruses or respiratory viruses. It is unclear whether the donor-derived virome in the transplanted lung influences recipient virome dynamics in other body compartments and if so, to which degree. Likewise, it is unknown whether dependencies exist among virus populations that mutually shape viral loads and kinetics. Results To address these questions, we characterized viral communities in airways and plasma of 49 LTRs and analyzed their abundance patterns in a data modeling approach. We found distinct viral clusters that were specific for body compartments and displayed independent dynamics. These clusters robustly gathered specific viral species across the patient cohort. In the lung, viral cluster abundance associated with time after transplantation and we detected mutual exclusion of viral species within the same human host. In plasma, viral cluster dynamics were associated with the indication for transplantation lacking significant short-time changes. Interestingly, pathogenic viruses in the plasma co-occurred specifically with Alpha torque virus genogroup 4 and Gamma torque virus strains suggesting shared functional or ecological requirements. Conclusions In summary, the detailed analysis of virome dynamics after lung transplantation revealed host, body compartment, and time-specific dependency patterns among viruses. Furthermore, our results suggested genetic adaptation to the host microenvironment at the level of the virome and support the hypothesis of functional complementarity between Anellovirus groups and other persistent viruses.

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“…Studies have revealed that microorganisms of the genus Sporosarcina can usually be found in bronchial biopsies of children with cystic fibrosis 35 . The Nicod team also reported that Porphyromonas, Rothia, Streptococcus , Neisseria, and other microorganisms are the principal constitutive members in the lungs of healthy individuals 36 . It can be seen that our research results are basically consistent with existing reports that indicate that RS-5645 not only alleviates the organ damage caused by inflammatory-factor storms, but also regulates the microbial flora of the lungs, allows pathogenic flora to revert to normalized flora, and promotes organ repair and micro-ecologic balance.…”

Section: Discussionmentioning

confidence: 99%

RS-5645 attenuates inflammatory cytokine storm induced by SARS-CoV-2 spike protein and LPS by modulating pulmonary microbiota

Liu¹,

Wu²,

Han³

et al. 2021

Int. J. Biol. Sci.

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An inflammatory cytokine storm is considered an important cause of death in severely and critically ill COVID-19 patients, however, the relationship between the SARS-CoV-2 spike (S) protein and the host's inflammatory cytokine storm is not clear. Here, the qPCR results indicated that S protein induced a significantly elevated expression of multiple inflammatory factor mRNAs in peripheral blood mononuclear cells (PBMCs), whereas RS-5645 ((4-(thiophen-3-yl)-1-(p-tolyl)-1H-pyrrol-3-yl)(3,4,5-trimethoxyphenyl)methanone) attenuated the expression of the most inflammatory factor mRNAs. RS-5645 also significantly reduced the cellular ratios of CD45+/IFNγ+, CD3+/IFNγ+, CD11b+/IFNγ+, and CD56+/IFNγ+ in human PBMCs. In addition, RS-5645 effectively inhibited the activation of inflammatory cells and reduced inflammatory damage to lung tissue in mice. Sequencing results of 16S rRNA v3+v4 in mouse alveolar lavage fluid showed that there were 494 OTUs overlapping between the alveolar lavage fluid of mice that underwent S protein+ LPS-combined intervention (M) and RS-5645-treated mice (R), while R manifested 64 unique OTUs and M exhibited 610 unique OTUs. In the alveoli of group R mice, the relative abundances of microorganisms belonging to Porphyromonas , Rothia , Streptococcus , and Neisseria increased significantly, while the relative abundances of microorganisms belonging to Psychrobacter , Shimia , and Sporosarcina were significantly diminished. The results of KEGG analysis indicated that the alveolar microbiota of mice in the R group can increase translation and reduce the activity of amino acid metabolism pathways. COG analysis results indicated that the abundance of proteins involved in ribosomal structure and biogenesis related to metabolism was augmented in the alveolar microbiota of the mice in the R group, while the abundance of proteins involved in secondary metabolite biosynthesis was significantly reduced. Therefore, our research results showed that RS-5645 attenuated pulmonary inflammatory cell infiltration and the inflammatory storm induced by the S protein and LPS by modulating the pulmonary microbiota.

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A prevalent and culturable microbiota links ecological balance to clinical stability of the human lung after transplantation

Cited by 42 publications

References 76 publications

Oral Prevotella Species and Their Connection to Events of Clinical Relevance in Gastrointestinal and Respiratory Tracts

Oral Prevotella Species and Their Connection to Events of Clinical Relevance in Gastrointestinal and Respiratory Tracts

Metagenomic sequencing reveals time, host, and body compartment-specific viral dynamics after lung transplantation

RS-5645 attenuates inflammatory cytokine storm induced by SARS-CoV-2 spike protein and LPS by modulating pulmonary microbiota

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