A previously unidentified sugar transporter for engineering of high-yield Streptomyces

Dong, Zhuoxu; Li, Lei; Du, Guozhong; Zhang, Yanyan; Wang, Xiangjing; Li, Shanshan; Xiang, Wensheng

doi:10.1007/s00253-023-12964-9

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…Recent studies have highlighted the role of membrane transporters in plant growth and development, as well as their adaptive pathways under abiotic stress [1,21,[190][191][192][193][194][195]. These studies have greatly improved our understanding of membrane transporters [196][197][198][199][200][201][202][203][204][205][206].…”

Section: Discussionmentioning

confidence: 99%

Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants

Yuan,

Wu,

Jiang

et al. 2024

Antioxidants

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Membrane transporters are proteins that mediate the entry and exit of substances through the plasma membrane and organellar membranes and are capable of recognizing and binding to specific substances, thereby facilitating substance transport. Membrane transporters are divided into different types, e.g., ion transporters, sugar transporters, amino acid transporters, and aquaporins, based on the substances they transport. These membrane transporters inhibit reactive oxygen species (ROS) generation through ion regulation, sugar and amino acid transport, hormone induction, and other mechanisms. They can also promote enzymatic and nonenzymatic reactions in plants, activate antioxidant enzyme activity, and promote ROS scavenging. Moreover, membrane transporters can transport plant growth regulators, solute proteins, redox potential regulators, and other substances involved in ROS metabolism through corresponding metabolic pathways, ultimately achieving ROS homeostasis in plants. In turn, ROS, as signaling molecules, can affect the activity of membrane transporters under abiotic stress through collaboration with ions and involvement in hormone metabolic pathways. The research described in this review provides a theoretical basis for improving plant stress resistance, promoting plant growth and development, and breeding high-quality plant varieties.

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Section: Discussionmentioning

confidence: 99%

Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants

Yuan,

Wu,

Jiang

et al. 2024

Antioxidants

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“…Avermectin B 1a was extracted and analyzed as described previously [11]. The procedure for the extraction of filipin III was the same as the method for avermectin B 1a , and the concentration of filipin III was determined as previously described [27].…”

Section: Extraction and High-performance Liquid Chromatography (Hplc)...mentioning

confidence: 99%

“…S. avermitilis S0 is a high-yield avermectin-producing strain obtained via random mutation from S. avermitilis MA-4680 [11]. Genome analysis showed that there are two long inverted repeat segments in the flanking regions (left arm: 1 bp to 963,064 bp; right arm: 8,668,384 bp to 9,631,365 bp) in the S0 strain (Figure 1a).…”

Section: Analysis Of Avermectin and Filipin Biosynthesis In S Avermit...mentioning

confidence: 99%

“…For instance, the titer of avermectin B 1a of S. avermitilis has been enhanced through the manipulation of various transcription regulators, such as TetR family regulators SAV151 [5] and AveT [6], the MarR family regulator SAV4189 [7] and the sigma factor σ hrdB [8]. Titer improvement could also be achieved by engineering transporters for carbon substrate uptake (e.g., malEFG, TP2, TP5 and TP6568) and putative product efflux (e.g., AvtAB and MiltAB2) [9][10][11][12][13]. Moreover, the titer of avermectin B 1a was boosted by approximately 50% to 9.31 g/L using a triacylglycerol dynamic degradation strategy in our previous work [14], and Hao et al enhanced the titer of avermectin B 1a to 9.61 g/L through a combination engineering strategy [15].…”

Section: Introductionmentioning

confidence: 99%

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Influence of Cluster-Situated Regulator PteF in Filipin Biosynthetic Cluster on Avermectin Biosynthesis in Streptomyces avermitilis

Du,

Yang,

et al. 2024

Biology

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Crosstalk regulation is widespread in Streptomyces species. Elucidating the influence of a specific regulator on target biosynthetic gene clusters (BGCs) and cell metabolism is crucial for strain improvement through regulatory protein engineering. PteF and PteR are two regulators that control the biosynthesis of filipin, which competes for building blocks with avermectins in Streptomyces avermitilis. However, little is known about the effects of PteF and PteR on avermectin biosynthesis. In this study, we investigated their impact on avermectin biosynthesis and global cell metabolism. The deletion of pteF resulted in a 55.49% avermectin titer improvement, which was 23.08% higher than that observed from pteR deletion, suggesting that PteF plays a more significant role in regulating avermectin biosynthesis, while PteF hardly influences the transcription level of genes in avermectin and other polyketide BGCs. Transcriptome data revealed that PteF exhibited a global regulatory effect. Avermectin production enhancement could be attributed to the repression of the tricarboxylic acid cycle and fatty acid biosynthetic pathway, as well as the enhancement of pathways supplying acyl-CoA precursors. These findings provide new insights into the role of PteF on avermectin biosynthesis and cell metabolism, offering important clues for designing and building efficient metabolic pathways to develop high-yield avermectin-producing strains.

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Application and research progress of ARTP mutagenesis in actinomycetes breeding

Zhu,

Ding,

Rang

et al. 2024

Gene

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A previously unidentified sugar transporter for engineering of high-yield Streptomyces

Cited by 4 publications

References 48 publications

Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants

Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants

Influence of Cluster-Situated Regulator PteF in Filipin Biosynthetic Cluster on Avermectin Biosynthesis in Streptomyces avermitilis

Application and research progress of ARTP mutagenesis in actinomycetes breeding

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