A Primer on Gross Pathology Examination and Selected Images of Gross Specimens

Altaleb, Ahmad

doi:10.1007/978-3-030-53690-9_9

Cited by 2 publications

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“…However, each sample preparation step introduces a potential for variability in the resulting tissue preparations. For example, the specimens chosen to undergo histopathology are selected via macroscopic visual inspection and palpation 4 . As a result, the chosen samples may not be representative of the morphology of the bulk specimen and may exclude the most informative tissues.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)

Ecclestone

Hosseinaee

Abbasi

et al. 2021

Sci Rep

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Histological images are critical in the diagnosis and treatment of cancers. Unfortunately, current methods for capturing these microscopy images require resource intensive tissue preparation that may delay diagnosis for days or weeks. To streamline this process, clinicians are limited to assessing small macroscopically representative subsets of tissues. Here, a combined photoacoustic remote sensing (PARS) microscope and swept source optical coherence tomography system designed to circumvent these diagnostic limitations is presented. The proposed multimodal microscope provides label-free three-dimensional depth resolved virtual histology visualizations, capturing nuclear and extranuclear tissue morphology directly on thick unprocessed specimens. The capabilities of the proposed method are demonstrated directly in unprocessed formalin fixed resected tissues. The first images of nuclear contrast in resected human tissues, and the first three-dimensional visualization of subsurface nuclear morphology in resected Rattus tissues, captured with a non-contact photoacoustic system are presented here. Moreover, the proposed system captures the first co-registered OCT and PARS images enabling direct histological assessment of unprocessed tissues. This work represents a vital step towards the development of a rapid histological imaging modality to circumvent the limitations of current histopathology techniques.

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Section: Introductionmentioning

confidence: 99%

Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)

Ecclestone

Hosseinaee

Abbasi

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography

Ecclestone

Hosseinaee

Abbasi

et al. 2021

Translational Biophotonics: Diagnostics and Therapeutics

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Histological images are critical in the diagnosis and treatment of cancers. Unfortunately, the current method for capturing these microscopy images require resource intensive tissue preparation that delays diagnosis for many days to a few weeks. To streamline this process, clinicians are limited to assessing small macroscopically representative subsets of tissues. Here, we present a combined photoacoustic remote sensing (PARS) microscope and swept source optical coherence tomography (SS-OCT) system designed to circumvent these diagnostic limitations. The proposed multimodal microscope provides label-free three-dimensional depth resolved virtual histology visualizations, capturing nuclear and extranuclear tissue morphology directly on thick unprocessed specimens. The capabilities of the proposed method are demonstrated directly in unprocessed formalin fixed resected tissues. Here, we present the first images of nuclear contrast in resected human tissues, and the first 3-dimensional visualization of subsurface nuclear morphology in resected Rattus tissues, captured with a non-contact photoacoustic system. Moreover, we present the first co-registered OCT and PARS images enabling direct histological assessment of unprocessed tissues. This work represents a vital step towards the development of a real-time histological imaging modality to circumvent the limitations of current histopathology techniques.

show abstract

A Primer on Gross Pathology Examination and Selected Images of Gross Specimens

Cited by 2 publications

References 0 publications

Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)

Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography (OCT)

Three-dimensional virtual histology in unprocessed resected tissues with photoacoustic remote sensing (PARS) microscopy and optical coherence tomography

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