A Pro23His Mutation Alters Prenatal Rod Photoreceptor Morphology in a Transgenic Swine Model of Retinitis Pigmentosa

Scott, Patrick A.; Castro, Juan P. Fernandez de; Kaplan, Henry J.; McCall, Maureen A.

doi:10.1167/iovs.13-13723

Cited by 26 publications

(41 citation statements)

References 37 publications

(51 reference statements)

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“…28 The diet was continued until sacrifice at E112, two days prior to parturition. A second domestic sow was inseminated and fed the same diet without curcumin supplementation and her embryos served as untreated controls.…”

Section: Administration Of Curcuminmentioning

confidence: 99%

“…28 Eyes were enucleated and immediately immersed in fixative (4% paraformaldehyde for immunohistochemistry and light microscopy; 2% paraformaldehyde/2% glutaraldehyde for transmission electron microscopy) for 24 hours at 48C. The results are from a total of 40 eyes (20 embryos total).…”

Section: Retinal Morphologymentioning

confidence: 99%

“…28 Briefly, a 3-mm wide vertical band of retinal tissue extending from the optic disc to the ora serrata was dissected from the superior retina of the right eye from all embryos and processed for examination at the light microscopic level. Sections were examined at 340 or 3100 using a NIKON EFD-3 Episcopic-Fluorescence microscope (Nikon Inc., Melville, NY).…”

Section: Morphometric Analysis Of the Retinamentioning

confidence: 99%

“…18 Curcumin, administered from P30 to P70, inhibited severe retinal rod PR degeneration in transgenic P23H rats, which was correlated with dissociated mutant protein aggregation and translocation of mutant rhodopsin to the appropriate cellular compartment. Aggregation and mistrafficking of mutant rhodopsin protein, common across RP models, [19][20][21][22][23][24][25][26][27][28] is thought to be cytotoxic. Thus, administration prior to these events could prove an effective adjunctive therapy to other strategies that attempt to alter mutant rhodopsin expression.…”

Section: Introductionmentioning

confidence: 99%

“…We sought to determine if curcumin administration would have similar neuroprotective effects in a large animal model of RP, which exhibits functional and morphological characteristics similar to P23H retinopathy in man. 28,29,30 Because alterations in morphology arise prenatally in this swine RP model, we exposed the embryos prenatally to curcumin. Our results show that curcumin delays structural defects in transgenic P23H miniswine embryo rod PRs.…”

Section: Introductionmentioning

confidence: 99%

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Prenatal Exposure to Curcumin Protects Rod Photoreceptors in a Transgenic Pro23His Swine Model of Retinitis Pigmentosa

Scott

Kaplan

McCall

2015

Trans. Vis. Sci. Tech.

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Purpose: Rhodopsin localization and rod photoreceptor (PR) morphology is altered in embryonic transgenic (Tg) Pro23His (P23H) miniswine. At birth, the Tg P23H swine retina lacks rod driven signaling. Curcumin, a neuroprotective food additive, has been shown to rescue Tg P23H rat rod PRs and promote normal trafficking of rhodopsin. We tested the hypothesis that prenatal exposure to curcumin would prevent PR morphological changes in Tg P23H miniswine retinae.Methods: A domestic sow was inseminated with semen from a Tg P23H miniswine founder. Her daily diet was supplemented with curcumin (100 mg/Kg body weight) from embryonic (E) day 80 to E112. The same diet without curcumin was fed to a second inseminated control sow. At E112, 2 days before parturition, both sows were euthanized. Their embryos were harvested, genotyped, and their eyes enucleated and prepared for morphological evaluation.Results: In all pigs, we measured mean outer retinal thickness, localization of rhodopsin, and rod PR morphology. Curcumin-treated Tg P23H swine embryonic retinas were similar to WT. Untreated Tg P23H embryonic retinas show significant degenerative effects; their outer retina was thinner, rod PR morphology was abnormal, and rhodopsin was mislocalized to the outer nuclear layer (ONL).Conclusions: These data support a role for curcumin as a neuroprotective agent that prevents/delays morphological abnormalities associated with rod PR degeneration in this Tg P23H swine model of retinitis pigmentosa (RP).

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Section: Administration Of Curcuminmentioning

confidence: 99%

Section: Retinal Morphologymentioning

confidence: 99%

Section: Morphometric Analysis Of the Retinamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prenatal Exposure to Curcumin Protects Rod Photoreceptors in a Transgenic Pro23His Swine Model of Retinitis Pigmentosa

Scott

Kaplan

McCall

2015

Trans. Vis. Sci. Tech.

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Anatomic Studies of the Miniature Swine Cornea

Abhari

Eisenback

Kaplan

et al. 2018

The Anatomical Record

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The domestic swine eye resembles the human eye both anatomically and physiologically. Xenotransplantation of the swine cornea to humans in need of full keratoplasty shows promise as a potential therapeutic strategy to restore vision in individuals with advanced corneal disease, especially those residing in developing nations. That said, we characterized the morphology of corneas from miniature swine, which are smaller in size, easier to handle, and more cost-effective compared to domestic swine. Eyes (N = 15) were harvested from miniature swine from different age groups: 1 month (N = 3), 2 month (N = 3), 4 month (N = 3), 8 month (N = 3), as well as 24 month old adult domestic swine (N = 3). They were immediately submerged in fixative and processed for histological examination at the light and transmission electron microscopic level. Gross anatomic measurements of the cornea were significantly less (P value ≤ 0.05) in miniature swine versus domestic swine. Corneal strata exhibited morphological characteristics similar to the domestic swine cornea. Adult miniature swine corneas show similar overall corneal thickness at 8 months of age versus domestic swine. Miniature swine exhibit similar corneal morphology with the domestic pig and humans, with the exception of Bowman’s layer, which is absent in pigs. Therefore, miniature pigs may be a useful resource of corneal tissue for humans in need of full keratoplasty, as well as serve as a large eye model for ophthalmology residents to develop surgical skills and for development and testing of ocular therapeutic strategies that translate to humans.

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Gross anatomy, histological, and histochemical analysis of the eyelids and orbital glands of the neonate pygmy hippopotamus (Suina: Choeropsis liberiensis or Hexaprotodon liberiensis, Morton 1849) with reference to its habitat

Klećkowska‐Nawrot

Goździewska‐Harłajczuk

Paszta

2020

The Anatomical Record

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The pygmy hippopotamus is phylogenetically related to members of both the Suidae and Cetacea. However, differences in their habitats may have resulted in variation in the anatomy and physiology of the ocular adnexa between these species. Therefore, this study focuses on the identification of accessory organs of the eye, which are typical for the pygmy hippopotamus and are comparable to organs present in mammals related to it. Moreover, the secretions produced by the superficial gland of the third eyelid, the deep gland of the third eyelid and the lacrimal gland were examined, as they ensure eyeball protection. In the upper and lower eyelids, numerous serous glands where identified, which were typical for the pygmy hippopotamus and similar as in the Cetacea. This study enabled to identify additional folds in the eyelids of the pygmy hippopotamus. Lymphoid follicles and diffuse lymphocytes were not found in the lymphoid region in the upper or lower eyelids and the third eyelid, which was most likely caused by the age of the studied hippopotamuses. An accurate histochemical analysis revealed that the secretions of the pygmy hippopotamus are very similar to the Sus scrofa. The structural differences between the pygmy hippopotamus and representatives of Cetacea are most likely caused by the fact that most of Cetacea live in saltwater and are exposed to more frequent fluctuations in water temperature compared to the pygmy hippopotamus, which lives in fresh water and does not lead a migratory lifestyle like the Cetacea.

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A Pro23His Mutation Alters Prenatal Rod Photoreceptor Morphology in a Transgenic Swine Model of Retinitis Pigmentosa

Cited by 26 publications

References 37 publications

Prenatal Exposure to Curcumin Protects Rod Photoreceptors in a Transgenic Pro23His Swine Model of Retinitis Pigmentosa

Prenatal Exposure to Curcumin Protects Rod Photoreceptors in a Transgenic Pro23His Swine Model of Retinitis Pigmentosa

Anatomic Studies of the Miniature Swine Cornea

Gross anatomy, histological, and histochemical analysis of the eyelids and orbital glands of the neonate pygmy hippopotamus (Suina: Choeropsis liberiensis or Hexaprotodon liberiensis, Morton 1849) with reference to its habitat

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