A probabilistic approach for the interpretation of RNA profiles as cell type evidence

Zoete, Jacob de; Curran, James M.; Sjerps, Marjan

doi:10.1016/j.fsigen.2015.09.007

Cited by 24 publications

(17 citation statements)

References 13 publications

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“…We constructed a probabilistic discrimination model for four types of body fluids based on seven selected microRNAs. This method might have applications in the forensic casework addressed in previous reports on mRNA-based body fluid identification 24,50 . In a trial, a jury could easily understand the result of the analysis of a stain and evaluate the evidence used in crime reconstruction.…”

Section: Discussionmentioning

confidence: 97%

Distinct spectrum of microRNA expression in forensically relevant body fluids and probabilistic discriminant approach

Fujimoto

Manabe

Morimoto

et al. 2019

Sci Rep

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MicroRNA is attracting worldwide attention as a new marker for the identification of forensically relevant body fluids. A probabilistic discriminant model was constructed to identify venous blood, saliva, semen, and vaginal secretion, based on microRNA expression assessed via RT-qPCR. We quantified 15 candidate microRNAs in four types of body fluids by RT-qPCR and found that miR-144-3p, miR-451a-5p, miR-888-5p, miR-891a-5p, miR-203a-3p, miR-223-3p and miR-1260b were helpful to discriminate body fluids. Using the relative expression of seven candidate microRNAs in each body fluid, we implemented a partial least squares-discriminant analysis (PLS-DA) as a probabilistic discriminant model and distinguished four types of body fluids. Of 14 testing samples, 13 samples were correctly identified with >90% posterior probability. We also investigated the effects of microRNA expression in skin, semen infertility, and vaginal secretion during different menstrual phases. Semen infertility and menstrual phases did not affect our body fluid identification system. Therefore, the selected microRNAs were effective in identifying the four types of body fluids, indicating that probabilistic evaluation may be practical in forensic casework.

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Section: Discussionmentioning

confidence: 97%

Distinct spectrum of microRNA expression in forensically relevant body fluids and probabilistic discriminant approach

Fujimoto

Manabe

Morimoto

et al. 2019

Sci Rep

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“…semen or vaginal epithelial cells) or if there are multiple contributors of the same gender present in the mixture. Probabilistic assessment at source level, that is, assigning an evidential weight to source testing results, has been described by Taylor et al [108], Taylor [109], De Wolff et al [110] and de Zoete et al [111,112].…”

Section: Source Identificationmentioning

confidence: 99%

DNA transfer in forensic science: A review

Oorschot

Szkuta

Meakin³

et al. 2019

Forensic Science International: Genetics

227

130

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Understanding the variables impacting DNA transfer, persistence, prevalence and recovery (DNA-TPPR) has become increasingly relevant in investigations of criminal activities to provide opinion on how the DNA of a person of interest became present within the sample collected. This review considers our current knowledge regarding DNA-TPPR to assist casework investigations of criminal activities. There is a growing amount of information available on DNA-TPPR to inform the relative probabilities of the evidence given alternative scenarios relating to the presence or absence of DNA from a specific person in a collected sample of interest. This information should be used where relevant. However, far more research is still required to better understand the variables impacting DNA-TPPR and to generate more accurate probability estimates of generating particular types of profiles in more casework relevant situations. This review explores means of achieving this. It also notes the need for all those interacting with an item of interest to have an awareness of DNA transfer possibilities post criminal activity, to limit the risk of contamination or loss of DNA. Appropriately trained forensic practitioners are best placed to provide opinion and guidance on the interpretation of profiles at the activity level. However, those requested to provide expert opinion on DNA-related activity level issues are often insufficiently trained to do so. We advocate recognition of DNA activity associated expertise to be distinct from expertise associated with the identification of individuals. This is to be supported by dedicated training, competency testing, authorisation, and regular fit for purpose proficiency testing. The possibilities for experts to report on activity-related issues will increase as our knowledge increases through further research, access to relevant data is enhanced, and tools to assist interpretations are better exploited. Improvement opportunities will be achieved sooner, if more laboratories and agencies accept the need to invest in these aspects as well as the training of practitioners.

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“…An interpretation method for these RNA cell typing tests has been developed based on present/absent scoring of RNA markers (Lindenbergh et al, 2013). These RNA test results may also be evaluated using a probabilistic approach (Zoete, Curran et al, 2015). Each marker is effectively a test for a certain cell type or group of cell types.…”

Section: Discussionmentioning

confidence: 99%

A probabilistic approach to body fluid typing interpretation: an exploratory study on forensic saliva testing

Wolff

Kal

Berger

et al. 2015

Law, Probability and Risk

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Identifying specific human body fluids and establishing their presence in traces can be crucial to help reconstructing alleged incidents in criminal cases. It is up to the forensic practitioner to test for the presence of body fluids, interpret the test results and draw scientifically supported conclusions that can be used in a court of law. This study presents a Bayesian network for the interpretation of test results for human saliva based on the presence of human salivary a-amylase. The Bayesian network can be used by forensic practitioners as an exploratory tool to form their expert opinion on the presence or absence of saliva in a trace.

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A probabilistic approach for the interpretation of RNA profiles as cell type evidence

Cited by 24 publications

References 13 publications

Distinct spectrum of microRNA expression in forensically relevant body fluids and probabilistic discriminant approach

Distinct spectrum of microRNA expression in forensically relevant body fluids and probabilistic discriminant approach

DNA transfer in forensic science: A review

A probabilistic approach to body fluid typing interpretation: an exploratory study on forensic saliva testing

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