A procedure for assessing the salt tolerance of lucerne (<i>Medicago sativa</i>L.) cultivar seedlings by combining agronomic and physiological indicators

Bao, Ai-Ke; Guo, Zhen; Zhang, Hongfei; Wang, Suo-Min

doi:10.1080/00288230909510525

Cited by 13 publications

(7 citation statements)

References 35 publications

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“…Morphological symptoms, biochemical and physiological responses of the wheat genotypes revealed that stress treatment with 200 mM NaCl for 14 days was most appropriate for comparative evaluation of the wheat genotypes ( Figure 1 and Supplementary Table S1). The available literature corroborated our finding as we found that 200 mM NaCl has been commonly used for evaluation of salt tolerance of different plant species including wheat (Tammam et al, 2008; Bao et al, 2009; Wu et al, 2013). Hence, salt treatment with 200 mM NaCl for 14 days was used in the subsequent experiments.…”

Section: Discussionsupporting

confidence: 89%

“…Relative value for the indicator in shoot and root was calculated from treatment (200 mM NaCl) mean values as percentages of the respective mean value under control condition (0 mM NaCl). The relative value for each of these parameters was then standardized according to the formulas given by Bao et al (2009); one for the indicators which are positively correlated (proline, soluble sugar, TPCs, and antioxidant potential) with salt tolerance [X′ ij = (X ij – X jmin )/(X jmax – X jmin )], and another for the indicators which are negatively correlated (DW, lipid peroxidation, Na + /K + ratio, and total chlorophyll content) with salt tolerance [X′ ij = 1 - (X ij – X jmin )/(X jmax – X jmin )]. Herein X′ ij = 0, when X ij = X jmin ; and X′ ij = 1, when X ij = X jmax .…”

Section: Methodsmentioning

confidence: 99%

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Physiological, Biochemical, Epigenetic and Molecular Analyses of Wheat (Triticum aestivum) Genotypes with Contrasting Salt Tolerance

et al. 2017

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Abiotic stress exerts significant impact on plant’s growth, development, and productivity. Productivity of crop plants under salt stress is lagging behind because of our limited knowledge about physiological, biochemical, epigenetic, and molecular mechanisms of salt tolerance in plants. This study aimed to investigate physio-biochemical, molecular indices and defense responses of selected wheat cultivars to identify the most contrasting salt-responsive genotypes and the mechanisms associated with their differential responses. Physio-biochemical traits specifically membrane stability index, antioxidant potential, osmoprotectants and chlorophyll contents, measured at vegetative stage, were used for multivariate analysis to identify the most contrasting genotypes. Genetic and epigenetic analyses indicated the possible mechanisms associated with differential response of the wheat genotypes under salt stress. Better antioxidant potential, membrane stability, increased accumulation of osmolytes/phytophenolics, and higher K+/Na+ ratio under 200 mM NaCl stress identified Kharchia-65 to be the most salt-tolerant cultivar. By contrast, increased MDA level, reduced soluble sugar, proline, total chlorophyll, total phenolics contents, and lower antioxidant potential in HD-2329 marked it to be sensitive to the stress. Genetic and bioinformatics analyses of HKT1;4 of contrasting genotypes (Kharchia-65 and HD-2329) revealed deletions, transitions, and transversions resulting into altered structure, loss of conserved motifs (Ser-Gly-Gly-Gly and Gly-Arg) and function in salt-sensitive (HD-2329) genotype. Expression analysis of HKTs rationalized the observed responses. Epigenetic variations in cytosine methylation explained tissue- and genotype-specific differential expression of HKT2;1 and HKT2;3.

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Physiological, Biochemical, Epigenetic and Molecular Analyses of Wheat (Triticum aestivum) Genotypes with Contrasting Salt Tolerance

et al. 2017

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“…where X ij is the j indicator value of i cultivar, X jmin is the minimum value of the j indicator and X jmax is the maximum value of the j indicator (Bao et al 2009;Wu et al 2013). The relative drought tolerance of sugar beet cultivars was then determined from fresh weight, water content, Na + and K + concentrations, Na + /K + ratio, and proline and sugar concentrations in the present study, presented as the following equation (Bao et al 2009;Wu et al 2013):…”

Section: Determination Of Osmotic Potential (ψ S ) and Calculation Ofmentioning

confidence: 99%

“…The relative drought tolerance of sugar beet cultivars was then determined from fresh weight, water content, Na + and K + concentrations, Na + /K + ratio, and proline and sugar concentrations in the present study, presented as the following equation (Bao et al 2009;Wu et al 2013):…”

Section: Determination Of Osmotic Potential (ψ S ) and Calculation Ofmentioning

confidence: 99%

Assessment of drought tolerance in seedlings of sugar beet (Beta vulgarisL.) cultivars using inorganic and organic solutes accumulation criteria

Wang

et al. 2014

Soil Science and Plant Nutrition

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Drought is a major environmental factor limiting the production of crops. Drought tolerance of sugar beet (Beta vulgaris L.) cultivars in terms of growth and inorganic and organic solute accumulation was analyzed in this study. Four-week-old seedlings of three cultivars, "Gantang7", "SD13829" and "ST21916", differing in drought tolerance, were treated with different degrees of drought stress. Plant shoots and roots were harvested at 5 d after stress and subjected to analysis. Drought significantly reduced shoot fresh weight and water content in the three cultivars, though the least reduction was found in "ST21916". Either decrease or no change in sodium (Na + ) and potassium (K + ) concentrations was observed in the three cultivars as an effect of drought, whereas a higher root Na + and a lower root K + concentration was noted in "Gantang7". Root calcium (Ca 2+ ) concentration significantly increased in "Gantang7" exposed to moderate and severe drought. Severe drought also caused a significant increase in the proline level in "SD13829" but not in "Gantang7" and "ST21916". Under drought stress, soluble sugar concentrations significantly increased in both "Gantang7" and "SD13829" whilst remaining unchanged in "ST21916". It is clear that Na + , K + and soluble sugars are major contributors to osmotic adjustment (OA), while Ca 2+ and proline have minor contributions to OA in all three cultivars. According to our comprehensive assessment of drought tolerance, it is shown that "ST21916" is more tolerant to drought than the other two cultivars. Therefore, our results suggest that "ST21916" may be more suitable for planting in arid and semi-arid regions.

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“…High biomass production, high nutritional quality and widespread adaptability have made alfalfa as a leading forage crop with a good fame of ‘Queen of the Forages’ (Bao et al, 2009; Kumar, 2011). As an economically important legume forage crop, alfalfa has brought considerable profits for local people and companies in northern China.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Stress Tolerance, Productivity, and Forage Quality in T1 Transgenic Alfalfa Co-overexpressing ZxNHX and ZxVP1-1 from Zygophyllum xanthoxylum

et al. 2016

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Salinization, desertification, and soil nutrient deprivation are threatening the production of alfalfa (Medicago sativa L.) in northern China. We have previously generated T0 transgenic alfalfa co-overexpressing Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 genes with enhanced salt and drought tolerance. To further develop this excellent breeding material into the new forage cultivar, stress tolerance, productivity, and forage quality of T1 transgenic alfalfa (GM) were assessed in this study. The GM inherited the traits of salt and drought tolerance from T0 generation. Most importantly, co-overexpression of ZxNHX and ZxVP1-1 enhanced the tolerance to Pi deficiency in GM, which was associated with more Pi accumulation in plants. Meanwhile, T1 transgenic alfalfa developed a larger root system with increased root size, root dry weight and root/shoot ratio, which may be one important reason for the improvement of phosphorus nutrition and high biomass accumulation in GM under various conditions. GM also accumulated more crude protein, crude fiber, crude fat, and crude ash than wild-type (WT) plants, especially under stress conditions and in the field. More interestingly, the crude fat contents sharply dropped in WT (by 66-74%), whereas showed no change or decreased less in GM, when subjected to salinity, drought or low-Pi. Our results indicate that T1 transgenic alfalfa co-overexpressing ZxNHX and ZxVP1-1 shows stronger stress tolerance, higher productivity and better forage quality. This study provides a solid foundation for creating the alfalfa cultivars with high yield, good quality and wide adaptability on saline, dry, and nutrient-deprived marginal lands of northern China.

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A procedure for assessing the salt tolerance of lucerne (Medicago sativaL.) cultivar seedlings by combining agronomic and physiological indicators

Cited by 13 publications

References 35 publications

Physiological, Biochemical, Epigenetic and Molecular Analyses of Wheat (Triticum aestivum) Genotypes with Contrasting Salt Tolerance

Physiological, Biochemical, Epigenetic and Molecular Analyses of Wheat (Triticum aestivum) Genotypes with Contrasting Salt Tolerance

Assessment of drought tolerance in seedlings of sugar beet (Beta vulgarisL.) cultivars using inorganic and organic solutes accumulation criteria

Assessment of Stress Tolerance, Productivity, and Forage Quality in T1 Transgenic Alfalfa Co-overexpressing ZxNHX and ZxVP1-1 from Zygophyllum xanthoxylum

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