A procedure for shoot organogenesis in vitro from leaves and nodes of an elite Eucalyptus gunnii clone: comparative histology

Hervé, Philippe; Jauneau, Alain; Pâques, M.; Marien, Jean-Noël; Böudet, Alain M.; Teulières, Chantal

doi:10.1016/s0168-9452(01)00451-4

Cited by 57 publications

(41 citation statements)

References 20 publications

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“…For clone 11, there was a higher number of callus and callus with buds when the leaf was divided into basal and apical portions, the basal portion yielding the best result, followed by the apical portion. These results are consistent with those observed on E. gunnii leaves that did not present cellular competence for bud regeneration when the region near the petiole was removed (Hervé et al, 2001). According to the authors, the regeneration was originated from meristems located in the leaf base and connected to the vascular bundles (Hervé et al, 2001).…”

Section: Resultssupporting

confidence: 91%

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla

Alcantara

Filho

Quoirin

2011

Sci. agric. (Piracicaba, Braz.)

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The hybrid Eucalyptus grandis × Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E. urophylla was studied in order to verify the effect of several factors: subculture duration on multiplication medium, type of explants, entire and half leaves: basal and apical portions, and duration of the culture on a regeneration medium. Differences in organogenic capacity of the two clones tested were observed. The explant most recommended for organogenesis is the basal section of the leaf collected from shoot clusters subcultured every 17 days. Moreover, the leaf explants must be transferred to a fresh bud induction medium every five days. This study also aimed at evaluating factors affecting the genetic transformation of leaf explants with the uidA gene, via co-cultivation with Agrobacterium tumefaciens, such as the pre-culture of the explants on a specific medium, the duration of their co-culture with the bacteria and the addition of acetosyringone to the culture media. The best conditions for the expression of the uidA gene were two days of pre-culture of the leaf tissues, three days of co-culture with the bacteria and the addition of acetosyringone in pre-and co-culture media. Key words: Agrobacterium tumefaciens, micropropagation, tissue culture, transgenic plant, woody species Organogênese e transformação genética transiente do híbridoEucalyptus grandis × Eucalyptus urophylla RESUMO: O híbrido Eucalyptus grandis × Eucalyptus urophylla apresenta alta produtividade e potencial para indústrias de papel, celulose e fibras. A organogênese de explantes foliares de dois clones de E. grandis × E. urophylla foi estudada para verificar fatores como tempo de repicagem das plântulas matrizes em meio de multiplicação; tipo de explantes, folhas inteiras e de meias-folhas (porções basais e apicais) e dos dias que os explantes foliares permaneceram em meio de regeneração. Foram observadas diferenças na capacidade organogênica dos dois clones testados. A parte basal das folhas, coletadas de brotações repicadas a cada 17 dias, apresentou superioridade organogênica. Explantes foliares devem ser transferidos para novo meio de indução de brotações a cada cinco dias. Este estudo também teve como objetivo avaliar fatores que afetam a transformação genética de explantes foliares com o gene uidA, via co-cultivo com Agrobacterium tumefaciens, tais como a pré-cultura, a duração da co-cultura e a adição da acetoseringona no meio de cultura. As melhores condições para a expressão do gene uidA foram a cada dois dias de pré-cultura de explantes foliares, três dias de co-cultura com a bactéria e a adição de acetoseringona nos meios de pré e co-cultura.

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Section: Resultssupporting

confidence: 91%

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla

Alcantara

Filho

Quoirin

2011

Sci. agric. (Piracicaba, Braz.)

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“…The base of the petiole was the best region for callogenesis and subsequent bud formation (Figure 2), probably because of the accumulation of photosynthesis products in the region, or to the polar transport of growth regulators (Margara, 1982). Results confirm those obtained by Muralidharan & Mascarenhas (1987) and Hervé et al (2001), who observed a correlation between the formation of red calluses and the regeneration of adventitious buds at distal part of the petiole of foliar explants of E. camaldulensis and E. gunnii, respectively.…”

Section: Effect Of the Combinations Of Naa And Bap On Callogenesis Ansupporting

confidence: 91%

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis

Dibax¹,

Eisfeld

Cuquel³

et al. 2005

Sci. agric. (Piracicaba, Braz.)

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Breeding methods based on genetic transformation techniques need to be implemented for Eucalyptus camaldulensis to shorten the long breeding cycles and avoid manipulation of adult trees; that requires the development of plant regeneration protocols enabling development of plants from transformed tissues. The present work aimed to optimise the regeneration process already established for the species. Cotyledonary leaves of E. camaldulensis were cultured in MS medium supplemented with naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) combinations. The most efficient treatment for bud indirect regeneration (2.7 µmol L -1 NAA and 4.44 µmol L -1 BAP) was used for further experiments. When explants were kept in the dark during the first 30 days, the percentage of explants forming calluses increased and explant necrosis was reduced in comparison with light-cultured explants. Mineral medium modifications were compared and half-strength MS mineral medium turned out to be as efficient as full-strength medium, producing 54% and 47% of explants with buds, respectively. For shoot elongation, MS medium with halfstrength nitrate and ammonium salts, and 0.2% activated charcoal yielded rooted shoots 1 to 8 cm high after one month. The procedure is an efficient protocol for E. camadulensis plant regeneration, reducing the stages necessary for the obtention of complete plants. Key words: in vitro culture, forest species, culture media, organogenesis REGENERAÇÃO DE PLANTAS DE Eucalyptus camaldulensis A PARTIR DAS EXPLANTES COTILEDONARESRESUMO: A implementação, para espécies florestais, de técnicas de melhoramento baseadas em métodos de transformação genética, permitirá reduzir os longos ciclos de melhoramento e evitar a manipulação de árvores adultas. Isto implica dispor de um protocolo de regeneração que permita o desenvolvimento de plantas a partir de tecidos transformados. Este trabalho teve como objetivo otimizar este protocolo de regeneração para Eucalyptus camaldulensis. Folhas cotiledonares foram cultivadas em meio de cultura MS suplementado com combinações de ácido naftalenoacético (ANA) e 6-benzilaminopurina (BAP). O tratamento mais eficiente em termos de regeneração indireta de gemas foi 2,7 µmol L -1 de ANA combinado com 4,44 µmol L -1 de BAP, o qual foi utilizado nos experimentos posteriores. A manutenção dos explantes no escuro durante os trinta primeiros dias elevou a porcentagem de explantes com calos e reduziu a morte dos explantes, em comparação com os que permaneceram na luz. Modificações da composição mineral do meio MS foram comparadas e mostraram que a redução de metade dos sais foi tão eficiente para a formação de gemas (54% dos explantes) quanto o meio completo (47%). O meio de cultura com a concentração de íons nitrato e amônio reduzida à metade e 0,2% de carvão ativado apresentou-se adequado para o alongamento e enraizamento das brotações que atingiram uma altura de 1 a 8 cm depois de 30 dias. O processo completo representa um protocolo eficiente para a regeneração de plantas de Eucalyptu...

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“…Picloram (i.e., 4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid) has been used alone at 20.7 µM for E. urophylla organogenesis [86] or at 0.04 µM in combination with 2.25 µM BA for E. gunnii Hook.f. organogenesis [129].…”

Section: Organogenesismentioning

confidence: 99%

Tissue Culture of Corymbia and Eucalyptus

Trueman

Hung

Wendling

2018

Forests

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Eucalypts are among the world's most widely planted trees, but the productivity of eucalypt plantations is limited by their often-low amenability to true-to-type propagation from cuttings. An alternative approach to cutting propagation is tissue culture, which can be used to micropropagate valuable genotypes rapidly while simultaneously preserving germplasm in vitro. This review describes the use of tissue culture methods such as shoot culture, organogenesis, and somatic embryogenesis for micropropagating eucalypts. This review also discusses the use of cool storage, encapsulation, and cryopreservation methods for preserving eucalypt germplasm and delaying tissue maturation under minimal-growth conditions.

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A procedure for shoot organogenesis in vitro from leaves and nodes of an elite Eucalyptus gunnii clone: comparative histology

Cited by 57 publications

References 20 publications

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis

Tissue Culture of Corymbia and Eucalyptus

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