A procedure to produce high alcohol intake in mice

Abstract

These data indicate that scheduling fluid intake produces high, stable EtOH consumption and BEC in male and female B6 and WSC mice.

Toward understanding the genetics of alcohol drinking through transcriptome meta-analysis

“…Two-bottle choice alcohol drinking is the most widely used model of voluntary consumption, but new models are emerging (30)(31)(32)(33). It would be interesting to determine the generality of our results to other measures of alcohol consumption.…”

Assessing appetitive and consummatory phases of ethanol self-administration in C57BL/6J mice under operant conditions: regulation by mGlu5 receptor antagonism

“…The amount of ethanol consumed (2.6 g kg −1 session −1 ) appears to be one of the highest levels of ethanol consumption observed in mice without further dietary manipulations (i.e., food or water restriction, or sucrose added to the solution; Finn et al 2005;Middaugh and Kelley 1999;Mittleman et al 2003;Ryabinin et al 2003). We attribute this high consumption to a combination of intermittent access (every second or third day) and access during the early dark phase, factors that have both been shown to enhance ethanol consumption in mice (Middaugh et al 2000b;Olive et al 2000;Rhodes et al 2005;Ryabinin et al 2003), but never previously in combination (as far as we can ascertain).…”

Accumbens neurochemical adaptations produced by binge-like alcohol consumption

“…While earlier SHAC studies allowed fluid access during the light phase of the circadian cycle Finn et al 2005), fluid was made available 3 h after onset of the dark phase in the present study because mice consume the majority of their daily food and fluid during the dark phase (Freund 1969;Goldstein and Kakihana 1977;Middaugh et al 1999;Kurokawa et al 2000). Despite modest fluid restriction to 3 h, SHAC mice in the present study consumed 1.3 to 2.7 g/kg alcohol from a 5% alcohol solution within 40 min.…”

“…Scheduled High Alcohol Consumption Mice were assessed for drinking using the SHAC paradigm Finn et al 2005), with the exception that alcohol/ water availability occurred during the dark phase of the light cycle. Tables 1 and 2 summarize the alcohol availability regimen.…”

“…However, alcohol consumption in the majority of rodent alcohol self-administration models rarely produces pharmacologically significant blood alcohol concentrations (BACs; i.e., >0.8 mg/ml blood) nor do animals maintain pharmacologically significant BACs over extended periods of alcohol access (e.g., Dole and Gentry 1984;Le et al 1994Le et al , 2001Grahame and Grose 2003). Recently, a partial model of alcoholism was developed in which mice consume between 1.5-2 g/kg alcohol within a 30-min period over multiple alcohol presentations Finn et al 2005), resulting in BACs above 100 mg% over multiple alcohol presentations, which produces signs of intoxication Finn et al 2005). Termed as Scheduled High Alcohol Consumption (SHAC), this model provides a simple tool to investigate the neurobiological consequences of repeated binge alcohol consumption in rodents.…”