A Progressive Somatic Cell Niche Regulates Germline Cyst Differentiation in the Drosophila Ovary

Shi, Jingyuan; Jin, Zhen; Yu, Yan; Fu, Yang; Huang, Huanwei; Cai, Tao; Xi, Rongwen

doi:10.1016/j.cub.2020.11.053

Cited by 28 publications

(40 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7). Such distinct functional domains would be consistent with the recently described functional compartmentalization of the somatic niche in the germarium of the Drosophila ovary (Shi et al, 2021; Tu et al, 2021) and the distinct populations of granulosa cells regulating reproductive onset vs duration in the mouse ovary (Niu and Spradling, 2020).…”

Section: Discussionsupporting

confidence: 87%

Somatic regulation of female germ cell regeneration and development in planarians

Khan

Newmark

2021

Preprint

View full text Add to dashboard Cite

SummaryFemale germ cells develop into oocytes, with the capacity for totipotency. In most animals, these remarkable cells are specified during development and cannot be regenerated. By contrast, planarians, known for their regenerative prowess, can regenerate germ cells. To uncover mechanisms required for female germ cell development and regeneration, we generated gonad-specific transcriptomes and identified genes whose expression defines progressive stages of female germ cell development. Strikingly, early female germ cells share molecular signatures with the pluripotent stem cells driving planarian regeneration. We uncovered spatial heterogeneity within somatic ovarian cells and found that a regionally enriched FoxL homolog is required for oocyte differentiation, but not specification, suggestive of functionally distinct somatic compartments. Unexpectedly, a neurotransmitter-biosynthetic enzyme, AADC, is also expressed in somatic gonadal cells, and plays opposing roles in female and male germ cell development. Thus, somatic gonadal cells deploy conserved factors to regulate germ cell development and regeneration in planarians.

show abstract

Section: Discussionsupporting

confidence: 87%

Somatic regulation of female germ cell regeneration and development in planarians

Khan

Newmark

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…For example, the terminal filament, cap, and anterior-escort cells act as a somatic niche for the GSCs ( Decotto and Spradling, 2005 ; Lin and Spradling, 1993 ; Wang and Page-McCaw, 2018 ; Xie and Spradling, 2000 ). Once GSCs divide to give rise to CBs, posterior escort cells guide CB differentiation by encapsulating the CB and the early-cyst stages ( Kirilly et al, 2011 ; Shi et al, 2021 ; Upadhyay et al, 2016 ). Follicle stem cells (FSCs), which are present towards the posterior of the germarium, divide and differentiate to give rise to follicle cells (FCs), which surround the late-stage cysts that give rise to egg chambers ( Margolis and Spradling, 1995 ; Nystul and Spradling, 2010 ; Rust et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Oo-site: A dashboard to visualize gene expression during Drosophila oogenesis suggests meiotic entry is regulated post-transcriptionally

et al. 2022

View full text Add to dashboard Cite

Determining how stem cell differentiation is controlled has important implications for understanding the etiology of degenerative disease and designing regenerative therapies. In vivo analyses of stem cell model systems have revealed regulatory paradigms for stem cell self-renewal and differentiation. The germarium of the female Drosophila gonad, which houses both germline and somatic stem cells, is one such model system. Bulk mRNA sequencing (RNA-seq), single-cell RNA-seq (scRNA-seq), and bulk translation efficiency (polysome-seq) of mRNAs are available for stem cells and their differentiating progeny within the Drosophila germarium. However, visualizing those data is hampered by the lack of a tool to spatially map gene expression and translational data in the germarium. Here, we have developed Oo-site (https://www.ranganlab.com/Oo-site), a tool for visualizing bulk RNA-seq, scRNA-seq, and translational efficiency data during different stages of germline differentiation, which makes these data accessible to non-bioinformaticians. Using this tool, we recapitulated previously reported expression patterns of developmentally regulated genes and discovered that meiotic genes, such as those that regulate the synaptonemal complex, are regulated at the level of translation.

show abstract

“…ICs express TJ and are located posterior to Cap Cells, which serve as adult niche cells for both GSCs and FSCs, and are already specified by the start of pupation. The most anterior ICs become regional subsets of ECs (r1 and r2a), which likely have distinctive roles in the adult [73,74]. These precursors markedly attenuate division, starting from the most anterior locations, during the second half of pupation (Table 2, Fig.…”

Section: Discussionmentioning

confidence: 99%

Adult stem cells and niche cells segregate gradually from common precursors that build the adult Drosophila ovary during pupal development

Reilein

Kogan

Misner

et al. 2021

eLife

View full text Add to dashboard Cite

Production of proliferative Follicle Cells (FCs) and quiescent Escort Cells (ECs) by Follicle Stem Cells (FSCs) in adult Drosophila ovaries is regulated by niche signals from anterior (Cap Cells, ECs) and posterior (polar FCs) sources. Here we show that ECs, FSCs and FCs develop from common pupal precursors, with different fates acquired by progressive separation of cells along the AP axis and a graded decline in anterior cell proliferation. ECs, FSCs and most FCs derive from Intermingled Cell (IC) precursors interspersed with germline cells. Precursors also accumulate posterior to ICs before engulfing a naked germline cyst projected out of the germarium to form the first egg chamber and posterior polar FC signaling center. Thus, stem and niche cells develop in appropriate numbers and spatial organization through regulated proliferative expansion together with progressive establishment of spatial signaling cues that guide adult cell behavior, rather than through rigid early specification events.

show abstract

A Progressive Somatic Cell Niche Regulates Germline Cyst Differentiation in the Drosophila Ovary

Cited by 28 publications

References 69 publications

Somatic regulation of female germ cell regeneration and development in planarians

Somatic regulation of female germ cell regeneration and development in planarians

Oo-site: A dashboard to visualize gene expression during Drosophila oogenesis suggests meiotic entry is regulated post-transcriptionally

Adult stem cells and niche cells segregate gradually from common precursors that build the adult Drosophila ovary during pupal development

Contact Info

Product

Resources

About