A Promising Candidate in Tendon Healing Events—PDGF-BB

Chen, Yixuan; Lyu, Kexin; Lu, Jingwei; Long, Longhai; Wang, Xiaoqiang; Liu, Tianzhu; Li, Sen

doi:10.3390/biom12101518

Cited by 15 publications

(10 citation statements)

References 85 publications

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“…For example, PDGF-AB promotes angiogenesis and reduces infarct size [77]. In a comprehensive review of MSK pathologies, Chen et al summarized the role of PDGF-BB in tendon pathologies; PDGF-BB has a positive effect on tendon healing by enhancing inflammatory responses, promoting angiogenesis, and increasing collagen synthesis and the biomechanics of the repaired tendon [78]. Interestingly, Kovacivic et al showed a dose-dependent response in angiogenesis using different recombinant PDGF-BB doses [79].…”

Section: Platelet-derived Growth Factor-bb (Pdgf-bb)mentioning

confidence: 99%

“…Many in vivo and in vitro studies have shown that different PRP constituents (e.g., platelet growth factors, cytokines, Angs, and various leukocytes) play pivotal effector roles in regulating angiogenesis, matrix formation, and cell proliferation during tissue repair [78]. Bertrand-Duchesne et al studied the mechanisms of various PDGF fractions in PRP that stimulate angiogenesis [125].…”

Section: In Vitro and Animal Studiesmentioning

confidence: 99%

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Angiogenesis and Tissue Repair Depend on Platelet Dosing and Bioformulation Strategies Following Orthobiological Platelet-Rich Plasma Procedures. A Narrative Review

Everts¹,

Lana²,

Onishi³

et al. 2023

Preprint

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Angiogenesis is new blood vessel formation from existing vessels and is a critical first step in tissue repair following chronic disturbances in healing and degenerative tissues. Chronic pathoanatomic tissues are characterized by a high number of inflammatory cells, overexpression of inflammatory mediators, such as tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1), the presence of mast cells, T cells, reactive oxygen species, and matrix metalloproteinases, and a decreased angiogenic capacity. Multiple studies have demonstrated that autologous orthobiological cellular preparations (e.g., platelet-rich plasma [PRP]) im-prove tissue repair and regenerate tissues. There are many PRP devices on the market. Unfortunately, they differ greatly in platelet numbers, cellular composition, and bioformulation. PRP is a platelet concentrate consisting of a high concentration of platelets with or without certain leukocytes, platelet-derived growth factors (PGFs), cytokines, molecules, and signaling cells. Several PRP products have immunomodulatory capacities that can influence resident cells in a diseased microenvironment, inducing tissue repair or re-generation. Generally, PRP is a blood-derived product, regardless of its platelet number and bioformulation, and the literature indicates both positive and negative patient treatment outcomes. Strangely, the literature does not designate specific PRP preparation qualifications that can potentially contribute to tissue re-pair. Moreover, the literature scarcely addresses the impact of platelets and leukocytes in PRP on (neo)angiogenesis other than a general one-size-fits-all statement that “PRP has angiogenic capabilities.” Here, we review the cellular composition of all PRP constituents, including leukocytes, and describe the importance of platelet dosing and bioformulation strategies in orthobiological applications to initiate angiogenic pathways that re-establish microvasculature networks, facilitating the supply of oxygen and nutrients to impaired tissues.

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Section: Platelet-derived Growth Factor-bb (Pdgf-bb)mentioning

confidence: 99%

Section: In Vitro and Animal Studiesmentioning

confidence: 99%

Angiogenesis and Tissue Repair Depend on Platelet Dosing and Bioformulation Strategies Following Orthobiological Platelet-Rich Plasma Procedures. A Narrative Review

Everts¹,

Lana²,

Onishi³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Among different strategies, growth factors have been extensively studied to support tendon healing. For example, platelet-derived growth factor-BB (PDGF-BB) is a promising candidate [ 8 ] and has been reported to improve the biomechanical outcome of a fully transected and sutured rabbit Achilles tendon three weeks post-surgery [ 9 ]. Transforming growth factor-β (TGF-β), as a further important growth factor during tendon healing [ 10 ], has been reported to be of favorable influence, particularly when applied at the interface to the bone [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Bioactive and Elastic Emulsion Electrospun DegraPol Tubes Delivering IGF-1 for Tendon Rupture Repair

Rieber

Meier-Bürgisser

Miescher

et al. 2023

IJMS

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Tendon injuries can result in two major drawbacks. Adhesions to the surrounding tissue may limit the range of motion, while fibrovascular scar formation can lead to poor biomechanical outcomes. Prosthetic devices may help to mitigate those problems. Emulsion electrospinning was used to develop a novel three-layer tube based on the polymer DegraPol (DP), with incorporated insulin-like growth factor-1 (IGF-1) in the middle layer. Scanning electron microscopy was utilized to assess the fiber diameter in IGF-1 containing pure DP meshes. Further characterization was performed with Fourier Transformed Infrared Spectroscopy, Differential Scanning Calorimetry, and water contact angle, as well as through the assessment of mechanical properties and release kinetics from ELISA, and the bioactivity of IGF-1 by qPCR of collagen I, ki67, and tenomodulin in rabbit Achilles tenocytes. The IGF-1-containing tubes exhibited a sustained release of the growth factor up to 4 days and showed bioactivity by significantly upregulated ki67 and tenomodulin gene expression. Moreover, they proved to be mechanically superior to pure DP tubes (significantly higher fracture strain, failure stress, and elastic modulus). The novel three-layer tubes intended to be applied over conventionally sutured tendons after a rupture may help accelerate the healing process. The release of IGF-1 stimulates proliferation and matrix synthesis of cells at the repair site. In addition, adhesion formation to surrounding tissue can be reduced due to the physical barrier.

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“…8−11 For example, lots of researchers analyze the concentration variation of the abnormal expression of PDGF-BB to discriminate the early stage, middle state, and final state of recurrent breast cancer in the growth and progression process. 12 To date, various methods have been explored for PDGF-BB detection, including enzyme-linked immunosorbent assay, 13 fluorescence, 14−17 colorimetry, 18−20 and electrochemical methods. 3 For instance, a fluorescence aptasensor was designed for PDGF-BB detection and extraction by a nanogel.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Platelet-derived growth factor-BB (PDGF-BB), a significant protein biomarker in recognition of tumors, − such as colorectal cancer and breast cancer, stimulates the division and proliferation of cancer cells. − Recently, numerous studies have demonstrated the close relationship between PDGF-BB and tumor vascular remodeling. − For example, lots of researchers analyze the concentration variation of the abnormal expression of PDGF-BB to discriminate the early stage, middle state, and final state of recurrent breast cancer in the growth and progression process . To date, various methods have been explored for PDGF-BB detection, including enzyme-linked immunosorbent assay, fluorescence, − colorimetry, − and electrochemical methods .…”

Section: Introductionmentioning

confidence: 99%

Self-Powered Biosensing System with Multivariate Signal Amplification for Real-Time Amplified Detection of PDGF-BB

Wang,

Yang

et al. 2023

Anal. Chem.

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A self-powered biosensing system with multivariate signal amplification is designed for the ultrasensitive, highly efficient, rapid-response, and real-time detection of platelet-derived growth factor-BB (PDGF-BB). The biosensing system is composed of enzymatic biofuel cells (EBFCs), a capacitor, a digital multimeter (DMM), and a computer. Using the hybridization chain reaction (HCR), a few single DNA chains are transformed into abundant double-helix chains, which stimulates the reduction of [Ru(NH 3 ) 6 ] 3+ to [Ru(NH 3 ) 6 ] 2+ by electrostatic interaction, corresponding to the "on" state for HCR. As a result, the open-circuit voltage (E OCV ) is significantly increased in this self-powered biosensing system. When PDGF-BB is present, a binding interaction between the target and the aptamer, i.e., PDGF-BB/Apt, corresponding to the "off" state for HCR, results in a decrease of E OCV . The PDGF-BB concentration is inversely proportional to E OCV , allowing readable, effective, and precise real-time detection of PDGF-BB. The detection limit of the biosensing system is 0.031 pg/mL (S/N = 3). This strategy provides a promising and powerful tool for the early clinical diagnosis of related colorectal cancer markers.

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A Promising Candidate in Tendon Healing Events—PDGF-BB

Cited by 15 publications

References 85 publications

Angiogenesis and Tissue Repair Depend on Platelet Dosing and Bioformulation Strategies Following Orthobiological Platelet-Rich Plasma Procedures. A Narrative Review

Angiogenesis and Tissue Repair Depend on Platelet Dosing and Bioformulation Strategies Following Orthobiological Platelet-Rich Plasma Procedures. A Narrative Review

Bioactive and Elastic Emulsion Electrospun DegraPol Tubes Delivering IGF-1 for Tendon Rupture Repair

Self-Powered Biosensing System with Multivariate Signal Amplification for Real-Time Amplified Detection of PDGF-BB

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