A proposed serotyping system for Flavobacterium psychrophilum

Abstract

Aims: To develop a common serological system for rapid and routine identification of the fish pathogen Flavobacterium psychrophilum. Methods: Thirty‐four isolates of Fl. psychrophilum from different fish species and different geographical areas were typed using a slide agglutination test and an enzyme‐linked immunosorbent assay (ELISA). Results: Seven host‐dependent serovars (1: salmon; 2: trout; 3: trout; 4: eel; 5: carp; 6: tench; 7: ayu) were found. Serovar 2 was divided into two antigenic subgroups (2a and 2b). The results achieved by both slide agglutination and ELISA methods were totally consistent with each other. Although both techniques proved to be simple to carry out and useful, only the ELISA allowed identification of Fl. psychrophilum serovars using unabsorbed antiserum and whole‐cells as antigens. Significance and Impact of the Study: This paper proposes a harmonized scheme for serological identification of Fl. psychrophilum to be used for diagnostic and seroepidemiological studies of the diseases it causes.

Phenotypic, serological and genetic characterization of Flavobacterium psychrophilum strains isolated from salmonids in Chile

“…Mata et al. (2002) proposed a serotyping system for F. psychrophilum using slide agglutination and compared it to the serological schemes previously proposed by Lorenzen & Olesen (1997) and Izumi & Wakabayashi (1999).…”

“…On the other hand, some typing methods have been used to differentiate F. psychrophilum strains for epizootiological studies such as serologic analysis (Wakabayashi et al. 1994; Madetoja, Hänninen, Hirvelä‐Koski, Dalsgaard & Wiklund 2001; Mata, Skarmeta & Santos 2002) as well as genotyping using random amplified polymorphic DNA (RAPD) (Chakroun, Urdaci, Faure, Grimont & Bernardet 1997), ribotyping and plasmid profiling (Chakroun, Grimont, Urdaci & Bernardet 1998; Madsen & Dalsgaard 2000; Madetoja et al. 2001), restriction fragment length polymorphism of PCR product (PCR‐RFLP) (Izumi, Aranishi & Wakabayashi 2003) and polymorphism in 16S rRNA genes (Soule, LaFrentz, Cain, LaPatra & Call 2005b).…”

“…1998; Urdaci et al. 1998; Faruk, Campbell, Thompson, Rangdale & Richards 2002; Mata et al. 2002; Soule, Cain, LaFrentz & Call 2005a; Ramsrud, LaFrentz, LaFrentz, Cain & Call 2007).…”

Assessment of cross‐protection to heterologous strains of Flavobacterium psychrophilum following vaccination with a live‐attenuated coldwater disease immersion vaccine

“…For effective development and commercialization of a vaccine to BCWD/RTFS it must be demonstrated that conferred protection is to a variety of bacterial strains. Although F. psychrophilum strain diversity continues to be characterized, it is known that at least seven F. psychrophilum serotypes exist (Dalsgaard & Madsen, ; Mata, Skarmeta, & Santos, ), and intraspecific genetic heterogeneity has been described using phage typing (Castillo, Espejo, & Middelboe, ), random amplified polymorphic DNA (RAPD) analysis (Chakroun, Grimont, Urdaci, & Bernardet, ), pulsed field gel electrophoresis (Arai, Morita, Izumi, Katagiri, & Kimura, ), restriction digestion/sequence analysis of ribosomal and housekeeping genes (Dalsgaard & Madsen, ), and MLST (Ngo et al., ; Nicolas et al., ; Van Vliet et al., ).…”

Flavobacterium psychrophilum y su patología en alevines de Onchorhynchus mykiss del centro piscícola El Ingenio, Huancayo

“…Actualmente, los análisis serológicos tipo aglutinación son de gran utilidad para el diagnóstico confirmativo de la enfermedad del agua fría, así como ensayos de anticuerpos fluorescentes (Holt 1994), pero se ha determinado la existencia de reacciones cruzadas con otras bacterias filamentosas como F. columnaris u otros grupos bacterianos (Faruk et al 2002;Mata et al 2002). Actualmente para muestras de campo existen varios métodos de identificación todos basados en técnicas moleculares como PCR y RFLP (Baliarda et al 2002;Nilsson & Strom 2002;del Cerro et al 2002a y b).…”