A prospective study on the microbiological examination of secretions from the paranasal sinuses in horses in health and disease

Gergeleit, Hauke; Verspohl, Jutta; Rohde, Judith; Rohn, Karl; Ohnesorge, Bernhard; Bienert‐Zeit, Astrid

doi:10.1186/s13028-018-0394-4

Cited by 10 publications

(25 citation statements)

References 28 publications

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“…Several of these cases had only aerobic culture performed which may account for negative culture results or absence of anaerobic bacteria . Our results mirror those of a recent prospective study examining the microbiologic features of samples acquired transendoscopically from the nasomaxillary aperture of healthy and diseased horses adding to the evidence that anaerobic bacteria should be suspected in cases of secondary sinusitis . Moreover, if culture results of a horse affected with sinusitis reveal an anaerobic infection, a complete evaluation to rule out dental disease or other cause of secondary sinusitis is warranted.…”

Section: Discussionsupporting

confidence: 78%

Culture‐independent and dependent evaluation of the equine paranasal sinus microbiota in health and disease

Beste

Lawhon

Chamoun‐Emanuelli

et al. 2019

Equine Veterinary Journal

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Summary Background Horses with bacterial sinusitis frequently undergo empirical treatment with antimicrobials, however, in some cases bacterial culture of the affected sinus is used to direct therapy. Data regarding which organisms are part of the commensal microbiota of the equine sinus are lacking making it difficult to interpret culture results and guide empiric antimicrobial selection. Objectives Our objectives were to describe the bacterial and fungal microbiota of the paranasal sinuses in clinically normal horses using culture‐dependent and independent approaches and to compare the bacterial culture and susceptibility patterns of normal horses with those from horses affected with primary and secondary sinusitis. Study design Experimental study and descriptive retrospective review of case records. Methods Sinus washes were collected from 23 healthy horses. Washes were submitted for routine culture and susceptibility testing and DNA was isolated for next generation sequencing of bacterial and fungal marker genes. For clinical cases of sinusitis, medical records from 2010 to 2017 were reviewed and horses diagnosed with primary and/or secondary sinusitis were included. Results The paranasal sinus cavity hosts multiple bacterial and fungal organisms. The bacterial microbiota of healthy horses consists largely of uncultivable, aerobic bacteria. While few anaerobes were isolated from normal horses, the majority of clinical cases resulted in growth of anaerobic organisms with no difference in the proportion of aerobic and anaerobic bacteria isolated from clinical cases. Main limitations Small sample size in both populations of horses and heterogeneity of the population prevent a more in‐depth analysis. Conclusions The microbiota of the paranasal sinuses of horses consists primarily of aerobic bacteria and fungal organisms, the majority of which are uncultivable via common clinical methods. Anaerobic bacteria are found in the majority of horses with clinical sinusitis. These findings suggest anaerobic bacteria are associated with sinusitis and their presence should be considered when treating horses with sinusitis.

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Section: Discussionsupporting

confidence: 78%

Culture‐independent and dependent evaluation of the equine paranasal sinus microbiota in health and disease

Beste

Lawhon

Chamoun‐Emanuelli

et al. 2019

Equine Veterinary Journal

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“…and Bacteroides ssp. were found to be the predominant bacteria genus present in odontogenic sinusitis cases [ 10 , 24 , 25 , 57 ]. The occurrence of obligate anaerobes also differs in primary sinusitis cases and healthy samples [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

“…were found to be the predominant bacteria genus present in odontogenic sinusitis cases [ 10 , 24 , 25 , 57 ]. The occurrence of obligate anaerobes also differs in primary sinusitis cases and healthy samples [ 57 ]. It is obvious that P. histicola may be able to also colonize this niche in the horse when invading from apical infections.…”

Section: Discussionmentioning

confidence: 99%

The facultative human oral pathogen Prevotella histicola in equine cheek tooth apical/ periapical infection: a case report

et al. 2021

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Background Prevotella histicola is a facultative oral pathogen that under certain conditions causes pathologies such as caries and periodontitis in humans. Prevotella spp. also colonize the oral cavity of horses and can cause disease, but P. histicola has not yet been identified. Case presentation A 12-year-old Tinker mare was referred to the clinic for persistent, malodorous purulent nasal discharge and quidding. Conservative antibiotic (penicillin), antiphlogistic (meloxicam), and mucolytic (dembrexine-hydrochloride) treatment prior to referral was unsuccessful and symptoms worsened. Oral examination, radiography, sino-/ rhinoscopy, and standing computed tomography revealed severe apical/ periapical infection of the upper cheek tooth 209 with accompanying unilateral sinonasal inflammation and conchal necrosis. The tooth exhibited extensive subocclusal mesial infundibular cemental hypoplasia and caries, and an occlusal fissure fracture. After mechanical debridement and thermoplastic resin filling of the spacious subocclusal carious infundibular lesion, the tooth was extracted intraorally. The sinusitis and conchal necrosis were treated transendoscopically. Selective bacteriological swab cultures of affected tooth roots and subsequent matrix-assisted laser desorption ionization-time of flight mass spectrometry showed an infection with the obligate anaerobic, Gram-negative bacterium P. histicola. Surgical intervention and adapted antibiotic therapy led to normal healing without complications. Conclusions This study provides the first documented case of dental infection in a horse caused by P. histicola at once indicating necessity of more sufficient microbiological diagnostics and targeted antibiotic treatment in equine dental practice. This finding is also conducive to understand species-specific Prevotella diversity and cross-species distribution.

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“…Neben Anaerobiern lassen sich auch Bakterien der Familie der Enterobakterien oft im Zusammenhang mit einer dentogenen Sinusitis nachweisen, wobei Escherichia coli einer der häufigeren Vertreter ist. Weitere häufig auftretende Eitererreger sind verschiedene Staphylokokkenund Streptokokkenarten [14].…”

Section: Dentogene Sinusitidenunclassified

“…Streptococcus equi ssp. zooepidemicus konnte zudem bei malignen Neoplasien und Sinuszysten isoliert werden [14]. Dies unterstreicht den opportunistischen Charakter dieses Bakteriums.…”

Section: Sekundäre Nicht Dentogene Sinusitidenunclassified

Sekrete aus den Nasennebenhöhlen des Pferdes – Untersuchungsmöglichkeiten und deren diagnostische Aussagekraft

Gergeleit¹,

Bienert‐Zeit²,

Ohnesorge³

2019

pferde spiegel

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A prospective study on the microbiological examination of secretions from the paranasal sinuses in horses in health and disease

Cited by 10 publications

References 28 publications

Culture‐independent and dependent evaluation of the equine paranasal sinus microbiota in health and disease

Culture‐independent and dependent evaluation of the equine paranasal sinus microbiota in health and disease

The facultative human oral pathogen Prevotella histicola in equine cheek tooth apical/ periapical infection: a case report

Sekrete aus den Nasennebenhöhlen des Pferdes – Untersuchungsmöglichkeiten und deren diagnostische Aussagekraft

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