A protein extract and a cysteine protease inhibitor enriched fraction from Jatropha curcas seed cake have in vitro anti-Toxoplasma gondii activity

Soares, Alexandra Martins dos Santos; Carvalho, Laís Pessanha de; Melo, Édesio José Tenório de; Costa, Helen Paula Silva da; Vasconcelos, Ilka M.; Oliveira, José T.A.

doi:10.1016/j.exppara.2015.03.011

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…from the Euphorbiaceae family has been used in many parts of the world for various medicinal purposes [ 20 ]. The leaf and latex extracts of J. curcas contained appreciable amounts of secondary metabolic compounds [ 21 - 23 ]. Leaves of this plant reported possessing antivirus on human immunodeficiency virus activity [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity of Jatropha curcas Linn. latex in cream formulation on CD68 expression in mice skin wound

et al. 2018

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Aim:The purpose of the present study was to determine the potential of Jatropha curcas latex in the cream formulation on CD68 immune expression (macrophages) during inflammatory phase wound healing process in mice skin.Materials and Methods:Amount of 12 two-months-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. The mice were allotted into four groups of each, entire surface of each group wound covered by base cream control, sulfadiazine 0.1% cream, J. curcas latex cream 10% and, 15%, respectively. All experiments were performed twice a day for 3 days. The wound healing was assayed in stained histological sections in immunohistochemical of the wounds. CD68 expression was investigated under a microscope.Results:The results showed that the cream from the 10% and 15% latex of J. curcas revealed moderate immune reaction to CD68 on wound healing.Conclusion:We concluded that the latex cream of J. curcas possesses anti-inflammatory activity in wound healing process of mice skin.

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Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity of Jatropha curcas Linn. latex in cream formulation on CD68 expression in mice skin wound

et al. 2018

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“…It has advantage over other solvents in stabilization of protein of interest. A 20-90 % ammonium sulphate saturation for Solanum aculeatissimum protease inhibitor was reported [17]. The low purification fold observed for Moringa oleifera seeds may be attributed to presence of activated protease enzyme or to some impurities interfering in this stage of purification, or it could also be due to stability factor.…”

Section: Discussionmentioning

confidence: 99%

“…Saline extract, which contain proteins from the latex of Moringa oleifera, was reported to have anticancer activities [16]. Other researchers used 0.01 N NaOH, distilled water [17] and 0.1 M phosphate buffer, pH 7.5 [9] as extraction media for various therapeutic proteins. Phosphate buffers at concentrations of 0.1-0.5 M are known for their solubilizing properties.…”

Section: Discussionmentioning

confidence: 99%

Partial Purification of Phytocystatin from Moringa oleifera lam

Abdulkadir¹,

Kabiru²,

Muhammad³

et al. 2022

AROC Pharm. Biotech.

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Background: Phytocystatins are plants cysteine protease inhibitors (CPIs) that are known for their numerous uses in medicine and biotechnology. Methods: Different extraction media which include, Sodium Hydroxide, Hydrochloric acid, Sodium Chloride, Sodium phosphate buffer and distilled Water were used to evaluate flower, leave, root, latex, and stem bark of Moringa oleifera for inhibitory activity against cysteine protease (Papain enzyme). The phosphate buffer extract with higher protease inhibitory activity was concentrated by cold acetone precipitation and further subjected to partial purification using ammonium sulphate fractionation and Hydrophobic chromatography on Phenyl Sepharose column. The molecular weight of partially purified protein was estimated by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis. Results: The extracts of Sodium phosphate buffer from the seeds of Moringa oleifera showed higher CPI activity of 11.23 units/mg protein. Cold acetone precipitated extract gave high yield of 94.6% protein with 60% inhibition of protease. The partially purified protein showed a fifty percent inhibitory concentration (IC50) of 1.22±0.3 mg/ml protein against the enzyme with 63% inhibition. An estimated molecular weight of 13.5kDa was obtained from electrophoretic analysis of partially purified protein. Conclusion: Moringa oleifera seeds could be a good source of low molecular weight protein Inhibitor of Cysteine protease and might be useful in biotechnology of traditional medicinal plants, in transgenic crops to arrest the negative pathogenic over expressions of cysteine proteases

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“…Soares et al [83] reported recently that a protein extract and a cysteine protease inhibitor enriched fraction (a unique protein band) from Jatropha curcas seed cake (used in folk medicine as laxative) were able to reduce a number of T. gondii infected cells and intracellular parasites.…”

Section: Compounds Of Natural Originmentioning

confidence: 98%