A proteomic study of the effects of ramipril on post‐infarction left ventricular remodelling in the rabbit

Abstract

Objectives: In this study, we used a proteomic approach to investigate the potential proteins regulated by ramipril in post-infarction left ventricular remodelling in the rabbit. Methods and results: Myocardial infarction (MI) was induced in male New Zealand White rabbits (2.5-3 kg) by ligation of the left anterior descending coronary artery. Two months later, the rabbits were either left untreated (MI group) or were treated daily for one month with 0.1 mg/kg wt of ramipril (ramipril group), then sacrificed. One month of ramipril treatment resulted in a significant improvement in the LV ejection fraction (LVEF) and a decrease in hydroxyproline content. The protein profiles of LV tissue showed that ramipril caused upregulation of glutathione peroxidase, superoxide dismutase (SOD), and heart-type fatty acid binding-protein (h-FABP) and downregulation of HSP27 and cyclophilin A. Ramipril treatment caused an increase in catalase, glutathione peroxidase, and SOD activity in the LV tissue. Oxidized glutathione levels and the GSSG/GSH ratio in the heart tissue were lower in the ramipril group than in the MI group. Conclusions: Ramipril increased antioxidative protein expression and enzyme activity, which could partly explain the role of ramipril in attenuating LV remodelling. In addition, the present study identifies several potential protein targets which may help to explain the mechanism by which ramipril exerts its effect in post-infarction LV remodelling in the rabbit.

A proteomic view of isoproterenol induced cardiac hypertrophy: Prohibitin identified as a potential biomarker in rats

“…However coronary ligation (MI and ramipril treatment) decreased the protein expression of H-FABP in the left ventricular tissue compared to sham group. In addition, ramipril (known for beneficial role in cardiovascular diseases) treatment caused an increase in H-FABP of rabbit LV with MI indicating that ramipril may modulate the expression of H-FABP by post translational modifications [23]. …”

Combination Angiotensin Converting Enzyme and Direct Renin Inhibition in Heart Failure following Experimental Myocardial Infarction

“…Although this study explored function and structure in detail, the mechanisms that might account for the observed benefits were not evaluated. We speculate that the addition of a DRI to ACE inhibition might further augment the expression of antioxidants, as recently demonstrated by proteomic analysis in the post‐MI setting [27].…”

Albusin B modulates lipid metabolism and increases antioxidant defense in broiler chickens by a proteomic approach

“…Jejunal protein profiles were studied using a proteomic approach. Tissue lysate was prepared and quantified following the procedure of Chen et al Isoelectric focusing (IEF) was carried out on an Ettan IPGphor isoelectric focusing system (Pharmacia Biotech, Uppsala, Sweden) using 13 cm IPG strips (pI 4–7). Protein (200 µg) was added to rehydration solution (7 mol L −1 urea, 2 mol L −1 thiourea, 4 g kg −1 CHAPS (3‐[(3‐cholamidopropyl)dimethylammonio]‐1‐propanesulfonate), 0.2 g kg −1 dithiothreitol, 0.5 g kg −1 Triton X‐100, and 0.4 g kg −1 carrier ampholytes) for 6 h; then IEF was performed for a total of 24 500 V h. Sodium dodecyl sulfate–polyacrylamide electrophoresis (SDS‐PAGE) on 12% gels was used for the second dimension.…”

“…The target protein spots were incubated in trypsin solution for 20 h at 37 °C as previously described . The tryptic peptides were subjected to mass spectrometric analysis.…”

“…Jejunal protein profiles were studied using a proteomic approach. Tissue lysate was prepared and quantified following the procedure of Chen et al 16 Isoelectric focusing (IEF) was carried out on an Ettan IPGphor isoelectric focusing system (Pharmacia Biotech, Uppsala, Sweden) using 13 cm IPG strips (pI 4-7). Protein (200 µg) was added to rehydration solution…”