A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection

Gong, Xue; Zhang, Yi-Bing

doi:10.1016/j.xpro.2022.101844

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…According to our previous reports, 14 , 50 zebrafish larvae (4 dpf) were transferred to petri dishes filled with SVCV solution, where SVCV was diluted in egg water to 5×10 6 TCID 50 /mL. 30 larvae at a time point were collected for RT-qPCR and 50 for western blotting.…”

Section: Methodsmentioning

confidence: 99%

“…Cell transfection was performed according to our previous reports. 15 , 50 Typically, cells seeded overnight were transfected with indicated plasmids which were diluted with Opti-MEM and mixed with polyethylenimine (PEI, molecular mass 25000 Da, Sigma-Aldrich), at a ratio of 1:3 (plasmids (μg)/PEI (μl)) for cultured fish cells and 1:5 for cultured HEK293T cells.…”

Section: Methodsmentioning

confidence: 99%

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Genome editing of FTR42 improves zebrafish survival against virus infection by enhancing IFN immunity

Qu,

Gong,

et al. 2024

iScience

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genome editing of FTR42 improves zebrafish survival against virus infection by enhancing IFN immunity

Qu,

Gong,

et al. 2024

iScience

Self Cite

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A finTRIM member 100 (FTR100) is unique to Otomorpha fish for constitutive regulation of IFN response

Guo

Cheng

Gong

et al. 2023

Water Biology and Security

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Zebrafish HERC7c Acts as an Inhibitor of Fish IFN Response

Gong

et al. 2023

IJMS

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In humans, four small HERCs (HERC3-6) exhibit differential degrees of antiviral activity toward HIV-1. Recently we revealed a novel member HERC7 of small HERCs exclusively in non-mammalian vertebrates and varied copies of herc7 genes in distinct fish species, raising a question of what is the exact role for a certain fish herc7 gene. Here, a total of four herc7 genes (named HERC7a–d sequentially) are identified in the zebrafish genome. They are transcriptionally induced by a viral infection, and detailed promoter analyses indicate that zebrafish herc7c is a typical interferon (IFN)-stimulated gene. Overexpression of zebrafish HERC7c promotes SVCV (spring viremia of carp virus) replication in fish cells and concomitantly downregulates cellular IFN response. Mechanistically, zebrafish HERC7c targets STING, MAVS, and IRF7 for protein degradation, thus impairing cellular IFN response. Whereas the recently-identified crucian carp HERC7 has an E3 ligase activity for the conjugation of both ubiquitin and ISG15, zebrafish HERC7c only displays the potential to transfer ubiquitin. Considering the necessity for timely regulation of IFN expression during viral infection, these results together suggest that zebrafish HERC7c is a negative regulator of fish IFN antiviral response.

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A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection

Cited by 3 publications

References 8 publications

Genome editing of FTR42 improves zebrafish survival against virus infection by enhancing IFN immunity

Genome editing of FTR42 improves zebrafish survival against virus infection by enhancing IFN immunity

A finTRIM member 100 (FTR100) is unique to Otomorpha fish for constitutive regulation of IFN response

Zebrafish HERC7c Acts as an Inhibitor of Fish IFN Response

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