A Pulsed Tangential-Flow Ultrafiltration Technique for Studying Protein-Drug Binding

Ghosh, Raja; Pan, Si; Wang, Lijuan; Lu, Simon

doi:10.1002/jps.23639

Journal of Pharmaceutical Sciences

2013

DOI: 10.1002/jps.23639

|View full text |Cite

A Pulsed Tangential-Flow Ultrafiltration Technique for Studying Protein-Drug Binding

Raja Ghosh

Si Pan

Lijuan Wang

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2015

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 39 publications

(68 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Studies on Drug – Human Serum Albumin Binding: The Current State of the Matter

Zhivkova

2015

CPD

View full text Add to dashboard Cite

Human serum albumin (HSA) is the major plasma protein with vital functions acting as depot and career for many endogenous (fatty acids, bilirubin, etc.) and exogenous substances (drugs, nutrients, etc.) in the blood. Binding to HSA controls the free, active concentration of the drug and may affect considerably the overall pharmacodynamic and pharmacokinetic profile. Studies on drug - protein binding are important from both theoretical and practical point of view as they allow better understanding of the processes underlying drug disposition and elimination and the effect of several pathological states or co-administered drugs on drug delivery and efficacy. The present review focuses on the current state of drug - HSA binding studies. The major functions and consequences of drug - protein binding are described. The X-ray structure of HSA is discussed focusing on the location and the architecture of the primary drug and fatty acids binding sites. Some of the most commonly used methods for drug - HSA binding assay are presented together with examples for their application. The most extensive studied topics in the area are discussed including quantitative characterization of drug - HSA complexation, identification of the binding sites, stereoselectivity of drug - HSA interactions, and thermodynamic characterization of the binding process. A short section is devoted to in silico prediction of drug - HSA binding as an important step in drug design and development.

show abstract

Studies on Drug – Human Serum Albumin Binding: The Current State of the Matter

Zhivkova

2015

CPD

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

A Pulsed Tangential-Flow Ultrafiltration Technique for Studying Protein-Drug Binding

Cited by 1 publication

References 39 publications

Studies on Drug – Human Serum Albumin Binding: The Current State of the Matter

Studies on Drug – Human Serum Albumin Binding: The Current State of the Matter

Contact Info

Product

Resources

About