A Purified Extract from <i>Clematis mandshurica</i> Prevents Adenoviral-TRAIL Induced Apoptosis on Rat Articular Chondrocytes

Lee, Sung Won; Lee, Hye Jeong; Moon, Jeong; Choi, So Ron; Kim, Duk Kyu; Kim, In Ryoung; Choi, Won Chul; Park, Bong Soo

doi:10.1142/s0192415x08005850

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…After activation, PI3K phosphorylates phosphatidylinositol (PIP) and generates PIP 3, thereby activating various downstream signaling pathways (80). Phosphorylation of PI3K/Akt inhibited the activity of Bax protein in chondrocytes and increased the expression of Bcl-2, thereby inhibiting apoptosis (81). Apoptosis and autophagy have an intricate relationship.…”

Section: Discussionmentioning

confidence: 99%

Inhibit inflammation and apoptosis of pyrroloquinoline on spinal cord injury in rat

Zhou¹,

Jin²,

Shi³

et al. 2021

Ann Transl Med

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Background: Pyrroloquinoline quinone (PQQ) is a redox cofactor that can participate in a variety of physiological and biochemical processes, such as anti-inflammatory, cytoprotection, anti-aging, and antiapoptosis. PQQ plays an important protective role in the central nervous system (CNS). However, the effects of PQQ on astrocytes of the CNS and spinal cord injury (SCI) of rats is still unclear. The present study investigates the role of PQQ in inflammation, apoptosis, and autophagy after SCI in rats. And the effect of PQQ on lipopolysaccharide (LPS)-induced apoptosis and inflammation of astrocytes in vitro, to explore the neuroprotective mechanism of PQQ.Methods: 60 specific pathogen free (SPF) SD male rats (200-250 g) were randomly divided into Normal group, Sham group, SCI group, and SCI + PQQ group, with 15 rats in each group. BBB score, HE staining, Nissl staining, Western blot, immunofluorescence, and other methods were used for detection.Results: Our results showed that PQQ could upregulate BBB score in SCI rats. In the second place, PQQ can increase the number and improve the morphology of neurons after SCI. The expression of IL-1β, TNF-α, IL-6 was significantly decreased after PQQ treatment. And then, the ratio of B-cell lymphoma-2 (Bcl-2)/Bcl-2 associated X protein (Bax) increased significantly, and the positive signal of NeuN increased obviously after PQQ treatment. There are a large number of co-localizations between Bcl-2 and NeuN.Meanwhile, PQQ could down-regulate the expression of Active-Caspase3, and PQQ treatment could reverse the transfer of Active-Caspase3/Caspase3 from the cytoplasm to the nucleus in neurons and astrocytes after SCI. At the same time, PQQ had no significant effect on the LC3b/a ratio. PQQ could decrease the LAMP2 expression in spinal cord after injury. The expression level of phospho-Akt (p-AKT) increased after SCI and decreased after PQQ treatment. In primary astrocytes, LPS could induce the expression levels of IL-1β, TNF-α, and IL-6, and which were inhibited by PQQ treatment at 12 hours. After treatment with LPS, the expression level of Active-Caspase3 increased, which could be reversed by PQQ treatment for 24 h.Conclusions: These results suggest that PQQ can ameliorate the motor function of hind limbs and the pathological changes of neurons and injured spinal cord after SCI, down-regulate the expressions of IL-1β, TNF-α, and IL-6, inhibit apoptosis after SCI, and inhibit LPS-induced apoptosis and inflammation of astrocytes.

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Section: Discussionmentioning

confidence: 99%

Inhibit inflammation and apoptosis of pyrroloquinoline on spinal cord injury in rat

Zhou¹,

Jin²,

Shi³

et al. 2021

Ann Transl Med

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“…[298][299][300][301][302][303] Extracts from Clematis vitalba exhibit antiinflammatory, antinociceptive, and antipyretic effects in induced osteoarthritis in mice. In vitro studies show SKI 306X or Clematis inhibits cartilage degradation, chondrocyte apoptosis, and production of tumor necrosis factor α and interleukin-1 β.…”

Section: Ski 306xmentioning

confidence: 99%

The Role of Chondroprotectants, Nutraceuticals, and Nutrition in Rehabilitation

Raditic¹,

Bartges²

2014

Canine Rehabilitation and Physical Therapy

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Protection of ginsenoside Rg1 on chondrocyte from IL-1β-induced mitochondria-activated apoptosis through PI3K/Akt signaling

Huang

Fan

2014

Mol Cell Biochem

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Chondrocyte apoptosis is closely related to the development and progression of osteoarthritis. Ginsenoside Rg1 protects cells by antagonizing apoptosis. This study aimed to investigate the protective effect of Rg1 on interleukin 1β (IL-1β)-induced chondrocyte apoptosis and the underlying molecular mechanisms. Chondrocytes were harvested from the joints of 1-week-old Sprague-Dawley rats. After treated with 10 μg/mL Rg1 for 2 h, the chondrocytes were cultured with 10 ng/mL IL-1β to induce cytotoxicity. Cell viability was assessed with MTT assays. Annexin V/propidium iodide staining and terminal deoxynucleotidyl transferase dUTP nick-end labeling were used to detect chondrocyte apoptosis. The contents of total Akt, phosphorylated Akt (p-Akt), Bcl-2, Bax, and cytochrome C (Cyt c) were determined by Western blotting assay. A quantitative colorimetric assay was used to determine caspase-3 activity. Our present findings have shown that pre-treatment of chondrocytes with Rg1 reduces IL-1β induced cytotoxicity/apoptosis. Rg1 pretreatment also decreases the activity of IL-1β that reduces expression of Bcl-2 and level of p-Akt, and increases Bax activity, Cyt c release, and caspase-3 activation. It also reverses the activity of IL-1β that reduces the expression of tissue inhibitor of metalloproteinase-1 expression and increased the synthesis of matrix metalloproteinase-13, with the net effect of inhibiting extracellular matrix degradation. These results indicate that Rg1 may protect chondrocytes from IL-1β-induced apoptosis via the phosphatidylinositol 3-kinase/protein kinase B signaling pathway, through preventing caspase-3 release.

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A Purified Extract from Clematis mandshurica Prevents Adenoviral-TRAIL Induced Apoptosis on Rat Articular Chondrocytes

Cited by 5 publications

References 27 publications

Inhibit inflammation and apoptosis of pyrroloquinoline on spinal cord injury in rat

Inhibit inflammation and apoptosis of pyrroloquinoline on spinal cord injury in rat

The Role of Chondroprotectants, Nutraceuticals, and Nutrition in Rehabilitation

Protection of ginsenoside Rg1 on chondrocyte from IL-1β-induced mitochondria-activated apoptosis through PI3K/Akt signaling

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