A putative adhesin gene cloned from Campylobacter jejuni

Kelle, K.; Pages, J.-M.; Bolla, Jean‐Michel

doi:10.1016/s0923-2508(99)80019-9

Cited by 21 publications

(16 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fauchère et al (1986) found that C. jejuni isolated from individuals with fever and diarrhoea exhibited greater binding to epithelial cells than strains isolated from individuals without fever and diarrhoea. Numerous studies have been done to identify and characterize C. jejuni adhesins (De Melo & Pechère, 1990;Fauchère et al, 1989;Jin et al, 2001;Kelle et al, 1998;Kervella et al, 1993;Konkel et al, 1997;McSweegan & Walker, 1986;Moser & Schröder, 1995;Moser et al, 1992Moser et al, , 1997Pei et al, 1998;Schröder & Moser, 1997). Based on these studies, C. jejuni appears to synthesize a number of adhesive molecules.…”

Section: Introductionmentioning

confidence: 99%

Maximal adherence and invasion of INT 407 cells by Campylobacter jejuni requires the CadF outer-membrane protein and microfilament reorganization

2003

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Maximal adherence and invasion of INT 407 cells by Campylobacter jejuni requires the CadF outer-membrane protein and microfilament reorganization

2003

View full text Add to dashboard Cite

“…The ability of C. jejuni to adhere to epithelial cells lining the gastrointestinal tracts of humans is proposed to be an important virulence attribute (2,4,8,10,11,15,(24)(25)(26)(27)32). This proposal is supported by data indicating that C. jejuni isolates recovered from individuals with fever and diarrhea adhere to cultured cells in greater numbers than isolates from asymptomatic individuals (5).…”

mentioning

confidence: 96%

“…The bestcharacterized C. jejuni adhesins include the 37-kDa outer membrane protein termed CadF (Campylobacter adhesion to fibronectin [Fn]) (13,15,16,22,23,39), a 42.3-kDa lipoprotein termed JlpA (jejuni lipoprotein A) (8), and a 28-kDa periplasmic/membrane-associated protein termed PEB1 (28). Other C. jejuni molecules that may act as adhesins include the flagellin proteins, lipopolysaccharide (20,25), the major outer membrane protein PorA (24,32), and P95 (10).…”

mentioning

confidence: 99%

Campylobacter jejuni Strains Compete for Colonization in Broiler Chicks

Konkel

Christensen

Dhillon

et al. 2007

Appl Environ Microbiol

View full text Add to dashboard Cite

Campylobacter jejuni isolates possess multiple adhesive proteins termed adhesins, which promote the organism's attachment to epithelial cells. Based on the proposal that one or more adhesins are shared among C. jejuni isolates, we hypothesized that C. jejuni strains would compete for intestinal and cecal colonization in broiler chicks. To test this hypothesis, we selected two C. jejuni strains with unique SmaI pulsed-field gel electrophoresis macrorestriction profiles and generated one nalidixic acid-resistant strain (the F38011 Nal r strain) and one streptomycin-resistant strain (the 02-833L Str r strain). In vitro binding assays revealed that the C. jejuni F38011 Nal r and 02-833L Str r strains adhered to LMH chicken hepatocellular carcinoma epithelial cells and that neither strain influenced the binding potential of the other strain at low inoculation doses. However, an increase in the dose of the C. jejuni 02-833L Str r strain relative to that of the C. jejuni F38011 Nal r strain competitively inhibited the binding of the C. jejuni F38011 Nal r strain to LMH cells in a dose-dependent fashion. Similarly, the C. jejuni 02-833L Str r strain was found to significantly reduce the efficiency of intestinal and cecal colonization by the C. jejuni F38011 Nal r strain in broiler chickens. Based on the number of bacteria recovered from the ceca, the maximum number of bacteria that can colonize the digestive tracts of chickens may be limited by host constraints. Collectively, these data support the hypothesis that C. jejuni strains compete for colonization in chicks and suggest that it may be possible to design novel intervention strategies for reducing the level at which C. jejuni colonizes the cecum.

show abstract

“…The Campylobacter jejuni strains 85H and 79AH were previously described (33). The C. coli strain 96C1 and 96C12 were kindly provided by Gwenolla Ermel (AFSSA-Ploufragan, France).…”

Section: Methodsmentioning

confidence: 99%