A putative G protein‐coupled receptor negatively controls sexual development in <i>Aspergillus nidulans</i>

Han, Kap-Hoon; Seo, Jeong‐Ah; Yu, Jae-Hyuk

doi:10.1111/j.1365-2958.2003.03940.x

Cited by 108 publications

(152 citation statements)

References 44 publications

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“…In our previous studies, we identified nine putative GPCRs in A. nidulans and showed that GprA and GprB are required for cleistothecia formation in selffertilization, but not in outcrosses (Han et al 2004a;Seo et al 2004). In these studies, we also demonstrated that GprA and GprB are not the GPCRs for FadA-mediated vegetative growth signaling.…”

Section: G203rmentioning

confidence: 69%

“…This can be explained by differential expression of multiple GPCRs and variation of coclustering effectors. We found that, while mRNA levels of all G-protein subunits are relatively constant throughout the life cycle, those of GPCRs vary (Figure 1, our unpublished data; Han et al 2004a;Seo et al 2004). Particularly, gprA and gprB were specifically expressed at 48 hr postsexual developmental induction .…”

Section: G203rmentioning

confidence: 91%

“…For Northern blot analyses, samples from vegetative growth and postdevelopmental induction cultures were collected as described (Han et al 2004a). Briefly, 5 3 10 7 conidia of relevant strains were inoculated in 100 ml liquid MM with 0.1% yeast extract in 250-ml flasks and incubated at 37°, 250 rpm.…”

Section: Methodsmentioning

confidence: 99%

“…Nucleic acid manipulation: Genomic DNA or total RNA isolation and Northern blot analyses were carried out as previously described (Seo et al 2003;Han et al 2004a). The DNA probes used to examine mRNA levels of gpgA were prepared by PCR amplification of the coding region of gpgA, using genomic DNA of FGSC4 as a template with OJA24 and OJA25 ( Table 2).…”

Section: Methodsmentioning

confidence: 99%

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Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Seo¹,

Han²,

2005

Genetics

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Vegetative growth signaling in the filamentous fungus Aspergillus nidulans is primarily mediated by the heterotrimeric G-protein composed of FadA (Ga), SfaD (Gb), and a presumed Gg. Analysis of the A. nidulans genome identified a single gene named gpgA encoding a putative Gg-subunit. The predicted GpgA protein consists of 90 amino acids showing 72% similarity with yeast Ste18p. Deletion (D) of gpgA resulted in restricted vegetative growth and lowered asexual sporulation. Moreover, similar to the DsfaD mutant, the DgpgA mutant was unable to produce sexual fruiting bodies (cleistothecia) in self-fertilization and was severely impaired with cleistothecial development in outcross, indicating that both SfaD and GpgA are required for fruiting body formation. Developmental and morphological defects caused by deletion of flbA encoding an RGS protein negatively controlling FadA-mediated vegetative growth signaling were suppressed by DgpgA, indicating that GpgA functions in FadA-SfaD-mediated vegetative growth signaling. However, deletion of gpgA could not bypass the need for the early developmental activator FluG in asexual sporulation, suggesting that GpgA functions in a separate signaling pathway. We propose that GpgA is the only A. nidulans Gg-subunit and is required for normal vegetative growth as well as proper asexual and sexual developmental progression.

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Section: G203rmentioning

confidence: 69%

Section: G203rmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Seo¹,

Han²,

2005

Genetics

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“…Briefly, using wild-type (FGSC4) genomic DNA as a template 59-and 39-flanking regions (1 kb) of sfgA were amplified with the primer pairs of OYG7-9 and OYG10-11, respectively. The argB 1 marker was amplified with OKH60-61 (Han et al 2004a). The three amplicons were fused as described .…”

Section: Methodsmentioning

confidence: 99%

FluG-Dependent Asexual Development in Aspergillus nidulans Occurs via Derepression

Seo¹,

2006

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The asexual spore is one of the most crucial factors contributing to the fecundity and fitness of filamentous fungi. Although the developmental activator FluG was shown to be necessary for activation of asexual sporulation (conidiation) and production of the carcinogenic mycotoxin sterigmatocystin (ST) in the model filamentous fungus Aspergillus nidulans, the molecular mechanisms underlying the developmental switch have remained elusive. In this study, we report that the FluG-mediated conidiation in A. nidulans occurs via derepression. Suppressor analyses of fluG led to the identification of the sfgA gene encoding a novel protein with the Gal4-type Zn(II) 2 Cys 6 binuclear cluster DNA-binding motif at the N terminus. Deletion (D) and 31 other loss-of-function sfgA mutations bypassed the need for fluG in conidiation and production of ST. Moreover, both DsfgA and DsfgA DfluG mutations resulted in identical phenotypes in growth, conidiation, and ST production, indicating that the primary role of FluG is to remove repressive effects imposed by SfgA. In accordance with the proposed regulatory role of SfgA, overexpression of sfgA inhibited conidiation and delayed/reduced expression of conidiation-and STspecific genes. Genetic analyses demonstrated that SfgA functions downstream of FluG but upstream of transcriptional activators (FlbD, FlbC, FlbB, and BrlA) necessary for normal conidiation.

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Implications of carbon catabolite repression for Aspergillus‐based cell factories: A review

Wang,

Jin

et al. 2024

Biotechnology Journal

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Carbon catabolite repression (CCR) is a global regulatory mechanism that allows organisms to preferentially utilize a preferred carbon source (usually glucose) by suppressing the expression of genes associated with the utilization of nonpreferred carbon sources. Aspergillus is a large genus of filamentous fungi, some species of which have been used as microbial cell factories for the production of organic acids, industrial enzymes, pharmaceuticals, and other fermented products due to their safety, substrate convenience, and well‐established post‐translational modifications. Many recent studies have verified that CCR‐related genetic alterations can boost the yield of various carbohydrate‐active enzymes (CAZymes), even under CCR conditions. Based on these findings, we emphasize that appropriate regulation of the CCR pathway, especially the expression of the key transcription factor CreA gene, has great potential for further expanding the application of Aspergillus cell factories to develop strains for industrial CAZymes production. Further, the genetically modified CCR strains (chassis hosts) can also be used for the production of other useful natural products and recombinant proteins, among others. We here review the regulatory mechanisms of CCR in Aspergillus and its direct application in enzyme production, as well as its potential application in organic acid and pharmaceutical production to illustrate the effects of CCR on Aspergillus cell factories.

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A putative G protein‐coupled receptor negatively controls sexual development in Aspergillus nidulans

Cited by 108 publications

References 44 publications

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

Multiple Roles of a Heterotrimeric G-Protein γ-Subunit in Governing Growth and Development of Aspergillus nidulans

FluG-Dependent Asexual Development in Aspergillus nidulans Occurs via Derepression

Implications of carbon catabolite repression for Aspergillus‐based cell factories: A review

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