A putative protein–RNA complex regulates posttranscriptional processing of cytochrome P450 aromatase (CYP19A1) in bovine granulosa cells

Sahmi, Fatiha; Sahmi, Malha; Gévry, Nicolas; Sahadevan, Pramod; Allen, Bruce G.; Price, Christopher A.

doi:10.1002/mrd.23289

Cited by 4 publications

(4 citation statements)

References 43 publications

(54 reference statements)

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“…One of the major characteristics is that the synthesis of estradiol by granulosa cells is related to follicle development, oocyte maturation, ovulation and inhibiting granulosa cell apoptosis [64,68]. Interestingly, the present results showed that milk-derived exosomes could promote the secretion of estradiol and progesterone in bovine granulosa cells.…”

Section: Discussionmentioning

confidence: 49%

Influence of Estrus on Dairy Cow Milk Exosomal miRNAs and Their Role in Hormone Secretion by Granulosa Cells

Liu

Nan

et al. 2023

IJMS

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Estrus is crucial for cow fertility in modern dairy farms, but almost 50% of cows do not show the behavioral signs of estrus due to silent estrus and lack of suitable and high-accuracy methods to detect estrus. MiRNA and exosomes play essential roles in reproductive function and may be developed as novel biomarkers in estrus detection. Thus, we analyzed the miRNA expression patterns in milk exosomes during estrus and the effect of milk exosomes on hormone secretion in cultured bovine granulosa cells in vitro. We found that the number of exosomes and the exosome protein concentration in estrous cow milk were significantly lower than in non-estrous cow milk. Moreover, 133 differentially expressed exosomal miRNAs were identified in estrous cow milk vs. non-estrous cow milk. Functional enrichment analyses indicated that exosomal miRNAs were involved in reproduction and hormone-synthesis-related pathways, such as cholesterol metabolism, FoxO signaling pathway, Hippo signaling pathway, mTOR signaling pathway, steroid hormone biosynthesis, Wnt signaling pathway and GnRH signaling pathway. Consistent with the enrichment signaling pathways, exosomes derived from estrous and non-estrous cow milk both could promote the secretion of estradiol and progesterone in cultured bovine granulosa cells. Furthermore, genes related to hormonal synthesis (CYP19A1, CYP11A1, HSD3B1 and RUNX2) were up-regulated after exosome treatment, while exosomes inhibited the expression of StAR. Moreover, estrous and non-estrous cow-milk-derived exosomes both could increase the expression of bcl2 and decrease the expression of p53, and did not influence the expression of caspase-3. To our knowledge, this is the first study to investigate exosomal miRNA expression patterns during dairy cow estrus and the role of exosomes in hormone secretion by bovine granulosa cells. Our findings provide a theoretical basis for further investigating milk-derived exosomes and exosomal miRNA effects on ovary function and reproduction. Moreover, bovine milk exosomes may have effects on the ovaries of human consumers of pasteurized cow milk. These differential miRNAs might provide candidate biomarkers for the diagnosis of dairy cow estrus and will assist in developing new therapeutic targets for cow infertility.

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Section: Discussionmentioning

confidence: 49%

Influence of Estrus on Dairy Cow Milk Exosomal miRNAs and Their Role in Hormone Secretion by Granulosa Cells

Liu

Nan

et al. 2023

IJMS

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“…tuberculosis bacilli and for the diagnosis of active TB [92]. RNA has a short half-life [93] and it is predicted to be found in only viable cells. In addition, M.…”

Section: Detection Of M Tuberculosismentioning

confidence: 99%

“…To minimize this possibility, real-time mRNA-based quantitative (q)RT-PCR, instead of DNA, might be useful to detect viable M. tuberculosis bacilli and for the diagnosis of active TB [ 92 ]. RNA has a short half-life [ 93 ] and it is predicted to be found in only viable cells. In addition, M. tuberculosis mRNA is quite stable, with a half-life of roughly 9 min [ 94 ].…”

Section: Detection Of M Tuberculosismentioning

confidence: 99%

Revisiting the methods for detecting Mycobacterium tuberculosis: what has the new millennium brought thus far?

Campelo

Sousa

Nogueira

et al. 2021

Access Microbiology

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Tuberculosis (TB) affects around 10 million people worldwide in 2019. Approximately 3.4 % of new TB cases are multidrug-resistant. The gold standard method for detecting Mycobacterium tuberculosis , which is the aetiological agent of TB, is still based on microbiological culture procedures, followed by species identification and drug sensitivity testing. Sputum is the most commonly obtained clinical specimen from patients with pulmonary TB. Although smear microscopy is a low-cost and widely used method, its sensitivity is 50–60 %. Thus, owing to the need to improve the performance of current microbiological tests to provide prompt treatment, different methods with varied sensitivity and specificity for TB diagnosis have been developed. Here we discuss the existing methods developed over the past 20 years, including their strengths and weaknesses. In-house and commercial methods have been shown to be promising to achieve rapid diagnosis. Combining methods for mycobacterial detection systems demonstrates a correlation of 100 %. Other assays are useful for the simultaneous detection of M. tuberculosis species and drug-related mutations. Novel approaches have also been employed to rapidly identify and quantify total mycobacteria RNA, including assessments of global gene expression measured in whole blood to identify the risk of TB. Spoligotyping, mass spectrometry and next-generation sequencing are also promising technologies; however, their cost needs to be reduced so that low- and middle-income countries can access them. Because of the large impact of M. tuberculosis infection on public health, the development of new methods in the context of well-designed and -controlled clinical trials might contribute to the improvement of TB infection control.

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“…One of the major characteristics is that the estradiol level within the dominant follicles and potential ovulatory follicles is higher compared with that of subordinate follicles and atretic follicles ( Ireland & Roche, 1982 ; Ireland & Roche, 1983 ). Estradiol synthesized by CYP19A1 in granulosa cells is related to follicle maturation and is essential for follicle development, ovulation, and inhibiting granulosa cell apoptosis ( Sahmi et al, 2019 ; Okamoto et al, 2016 ). In addition, inhibins, a negative feedback of FSH secretion, suppress the further development of follicles through the pituitary-gonadal system ( Xu et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin

Liu

Chen

et al. 2023

PeerJ

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Background Communication between oocytes and granulosa cells ultimately dictate follicle development or atresia. Melatonin is also involved in follicle development. This study aimed to investigate the effects of melatonin and its receptor antagonists on hormone secretion, as well as gene expression related to hormone synthesis, TGF-β superfamily, and follicle development in bovine granulosa cells, and assess the effects of melatonin in the presence of 4-P-PDOT and luzindole. Methods Bovine ovaries were collected from a local abattoir and follicular fluid (follicle diameter 5–8 mm) was collected for granulosa cell isolation and culture. Granulosa cells and culture medium were collected 48 h after treatment with melatonin at high dose concentrations (10−5 M) and low dose concentrations (10−9 M) in the absence/presence of 4-P-PDOT and luzindole (10−5 M or 10−9 M). Furthermore, the expression level of genes related to hormonal synthesis (CYP11A1, CYP19A1, StAR, and RUNX2), TGF-β superfamily (BMP6, INHA, INHBA, INHBB, and TGFBR3), and development (EGFR, DNMT1A, and FSHR) were detected in each experimental group by real-time quantitative PCR. In addition, the level of hormones in culture medium were detected using ELISA. Results Both 10−5 M and 10−9 M melatonin doses promoted the secretion of inhibin A and progesterone without affecting the production of inhibin B and estradiol. In addition, both promoted the gene expression of INHA, StAR, RUNX2, TGFBR3, EGFR, and DNMT1A, and inhibited the expression of BMP6, INHBB, CYP11A1, CYP19A1, and FSHR. When combined with different doses of 4-P-PDOT and luzindole, they exhibited different effects on the secretion of inhibin B, estradiol, inhibin A, and progesterone, and the expression of CYP19A1, RUNX2, BMP6, INHBB, EGFR, and DNMT1A induced by melatonin. Conclusion High and low dose melatonin receptor antagonists exhibited different effects in regulating hormone secretion and the expression of various genes in response to melatonin. Therefore, concentration effects must be considered when using luzindole or 4-P-PDOT.

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A putative protein–RNA complex regulates posttranscriptional processing of cytochrome P450 aromatase (CYP19A1) in bovine granulosa cells

Cited by 4 publications

References 43 publications

Influence of Estrus on Dairy Cow Milk Exosomal miRNAs and Their Role in Hormone Secretion by Granulosa Cells

Influence of Estrus on Dairy Cow Milk Exosomal miRNAs and Their Role in Hormone Secretion by Granulosa Cells

Revisiting the methods for detecting Mycobacterium tuberculosis: what has the new millennium brought thus far?

High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin

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