A pyrolysis mass spectrometry study of fusobacteria

Magee, J. T.; Hindmarch, J. M.; Bennett, K. W.; Duerden, B. I.; Aries, R. E.

doi:10.1099/00222615-28-3-227

Cited by 32 publications

(9 citation statements)

References 26 publications

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“…Mass 99 was eliminated as non-discriminatory in the Mahalanobis stepwise variate selection employed. The spectra were now represented by co-ordinates on 14 independent (orthogonal) discriminant function axes, accounting for c. 99% of inter-strain variance, and describing a statistical transformation that optimised inter-strain discrimination and corrected for withinstrain statistical effects (Magee et al, 1989). Spectrum co-ordinates on these axes were processed in classification analyses.…”

Section: Mathematical Analysesmentioning

confidence: 99%

A pyrolysis-mass spectrometry study of Corynebacterium spp.

Hindmarch

Magee²,

Hadfield³

et al. 1990

Journal of Medical Microbiology

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Section: Mathematical Analysesmentioning

confidence: 99%

A pyrolysis-mass spectrometry study of Corynebacterium spp.

Hindmarch

Magee²,

Hadfield³

et al. 1990

Journal of Medical Microbiology

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“…Whole-cell composition was examined by Py-MS in a Horizon Instruments PYMS 200X pyrolysis-mass spectrometer (Aries et al, 1986;Magee et al, 1989). Growth from 48-h cultures on blood agar was smeared on ironnickel alloy pyrolysis foils and heated rapidly in a vacuum to 530°C by the Curie point method.…”

Section: Pyrolysis-mass Spectrometry (Py-ms)mentioning

confidence: 99%

“…Spectra were normalised by the iterative technique of for further analysis as previously described (Magee et al, 1989); these comprised masses 51-60, 62-75, 77-89, 93-106, 108-1 13, 116, 118-128 and 132-136. Replicate spectra of each strain were labelled as a distinct group and normalised data for the selected peaks were subjected to discriminant analysis (SPSSX User's Guide, 1983).…”

Section: Pyrolysis-mass Spectrometry (Py-ms)mentioning

confidence: 99%

A Comparison of Bacteroides ureolyticus isolates from different clinical sources

Duerden

Eley

Goodwin

et al. 1989

Journal of Medical Microbiology

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Summary. Clinical isolates of corroding, gram-negative, anaerobic bacilli (provisionally identified as Bacteroides ureolyticus) from superficial ulcers and soft tissue infections (1 9, non-gonococcal, non-chlamydia1 urethritis (1 2) and adult periodontal disease (14) were compared with reference strains of B. ureolyticus, B. gracilis and Wolinella recta in a series of conventional tests of morphology, biochemical activity, tolerance of dyes and bile salts, and antibiotic sensitivity, gas-liquid chromatographic analysis of metabolic products, and in whole-cell analysis by pyrolysis-mass spectrometry (Py-MS). A numerical taxonomic approach was used with the results of conventional tests and the grouping obtained was compared with that obtained by Py-MS. All the ulcer and soft-tissue isolates and the urethritis isolates were oxidaseand urease-positive and formed a homogeneous set consistent with the reference strain of B. ureolyticus. The dental isolates differed from B. ureolyticus strains and were heterogeneous amongst themselves. None corresponded with the reference strains of B. gracilis or W . recta. The conventional and Py-MS approaches to c haracterisation produced similar groupings and each distinguished between a single cluster of ulcer-urethritis strains and several clusters of dental strains, although the dendrograms derived from the two approaches differed in the order of the clusters; in the Py-MS dendrogram one subcluster of four dental strains came within the main ulcer-urethritis cluster and a cluster of five ulcer strains was separated as a distinct group.

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“…There was over 90% agreement between PMS identification and that based on CTRPs, as in previous studies of Fusobacterium, 29 corynefor~ns~~ and the " Streptococcus milleri group ". 41 The only disagreement was in identification as Pr.…”

Section: Discussionmentioning

confidence: 49%

Classification of oral pigmented anaerobic bacilli by pyrolysis mass spectrometry and biochemical tests

Magee

Hindmarch²,

Duerden³

et al. 1992

Journal of Medical Microbiology

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Summary. Clinical (66) and reference (5) strains of pigmented gram-negative anaerobic bacilli, identified as Prevotella intermedia (47), Pr. melaninogenica ( 1 ), Pr. corpora (8), Porphyromonas asaccharolyticus (12), P. endodontalis (1) and P. gingivalis (2), were examined by pyrolysis mass spectrometry (PMS) and in conventional tests. Numerical classification based on conventional test reaction patterns (CTRPs) resolved five clusters, four comprising strains identified as Pr. intermedia, Pr. corpora, Pr. melaninogenica, and P . gingivalis respectively, and one comprising strains identified as P . asaccharolyticus and P . endodontalis. Numerical classification based on PMS showed a similar division, with decreasing homogeneity of chemical composition in the order Pr. intermedia, Pr. corpora, P. asaccharolyticus, which agreed with the order of homogeneity in CTRPs. PMS clusters corresponding to the genus Porphyromonas were clearly distinct from those of the genus Prevotella. PMS and CTRP classification disagreed on cluster membership for six strains. PMS identification from blind challenge sets was in agreement with conventional identification for 64 of 67 strains.

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A pyrolysis mass spectrometry study of fusobacteria

Cited by 32 publications

References 26 publications

A pyrolysis-mass spectrometry study of Corynebacterium spp.

A pyrolysis-mass spectrometry study of Corynebacterium spp.

A Comparison of Bacteroides ureolyticus isolates from different clinical sources

Classification of oral pigmented anaerobic bacilli by pyrolysis mass spectrometry and biochemical tests

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