1983
DOI: 10.1530/acta.0.1020153
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A quantitative assay for the cytoplasmic androgen receptor using [3H]dihydrotestosterone in the presence of NAD+-nucleosidase

Abstract: Ectomycorrhizal (EM) fleshy fungi are being monitored in a population of Fagus grandifolia var. mexicana persisting in a montane cloud forest refuge on a volcano in a subtropical region of central Veracruz (eastern Mexico). The population of Fagus studied represents one of the 10 recognized forest fragments still housing this tree genus in Mexico. This is the first attempt to document EM fungi associated with this tree species in Mexico. We present evidence of the ectomycorrhizal symbiosis for Lactarius badiop… Show more

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Cited by 7 publications
(5 citation statements)
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“…The cytosolic androgen receptor binding sites were measured as described before [18,20], according to the method of Wagner [35]. Prostates were homogenized after cooling in liquid nitrogen with a Microdismembrator (Braun/Melsungen, FRG).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The cytosolic androgen receptor binding sites were measured as described before [18,20], according to the method of Wagner [35]. Prostates were homogenized after cooling in liquid nitrogen with a Microdismembrator (Braun/Melsungen, FRG).…”
Section: Methodsmentioning
confidence: 99%
“…Protein was assayed by the method of Schaffner and Weissmann [30] with modifications as reported by Moeller et al [20]. DNA was assayed in the 100,000 g pellet according to the method of Burton [3] using deoxyribose as standard.…”
Section: Methodsmentioning
confidence: 99%
“…from the results presented in this work, and (4.) from several pharmacologic experiments changing the androgen receptors independently of circulating testosterone [9,12] and pituitary hormones [10,13,15].…”
Section: Diurnal Variationsmentioning
confidence: 99%
“…Cytosolic androgen receptor was analyzed according to Wagner [19] with the modifications of Moeller et al [8][9][10]. Briefly, the frozen tissue was powdered and suspended in 4 volumes of buffer (10 mM Tris-HC1, pH 7A, 0°C; 50 mM dithioerythritrol, 5 mM NAN3, 250 mM sucrose, 400 mU/ml NADase); i h centrifugation (100,000 g); incubation of supernatant for 24 h at 0°C with 20 nM 3H-DHT (final concentration) and additional 2 IxM R 1881 in parallel assays for identification of unspecifically bound 3H-DHT; separation of receptor-bound 3H-DHT by agar gel electrophoresis at low temperature [18].…”
Section: Receptor Analysismentioning
confidence: 99%