A Quantitative ELISA Using Monoclonal Antibody to Survey Paeoniilorin and Albiflorin in Crude Drugs and Traditional Chinese Herbal Medicines

Dissection of the role of paeoniflorin in the traditional Chinese medicinal formula Si-Ni-San against contact dermatitis in mice

“…Paeoniflorin with carrier protein conjugates was synthesized by a modified synthesis procedure (Lu et al, 2003). Paeoniflorin-bovine serum albumin conjugate was used the immunogen and applied in rabbits to get anti-paeoniflorin polyclonal antibodies, while paeoniflorin-ovalbumin conjugate was used as the coating antigen of enzymelinked immunosorbent assay (ELISA) for antiserum titer.…”

Quality Control by Immunoaffinity Concentration Using Monoclonal Antibody

“…Although immunoaffinity purification against higher molecule analyte such as peptides and proteins are widely used in the research and commercial ways, there are too few cases of immunoaffinity purification targeting a small molecule compound. Fortunately, in our ongoing studies of naturally occurring bioactive compounds we have prepared many kinds of MAbs against terpenoids (Sakata et al, 1994;Xuan et al, 1999;Lu et al, 2003), alkaloids Kim et al 2004), plant saponins www.intechopen.com…”

One-step immunochromatographic separation and ELISA quantification of glycyrrhizin from traditional Chinese medicines

“…Previously, we established a fast and sensitive assay system for the screening of GA concentration in large numbers of small samples with low concentration and for the quality control of drug production, pharmacological research and other applications [19][20][21]. As a second step, the purpose of our present work is to purify and quantify GA from two traditional Chinese medicines and crude drug extracts of licorice roots by means of one-step immunoaffinity chromatography using anti-GA monoclonal antibody (anti-GA-MAb) with detection by means of an enzyme-linked immunosorbent assay (ELISA).…”