A quantitative PCR assay for the detection and quantification of Babesia bovis and B. bigemina

Buling, A.; Criado–Fornelio, A.; Asenzo, G.; Benítez, D.; Barba-Carretero, J.C.; Florín-Christensen, Mónica

doi:10.1016/j.vetpar.2007.03.031

Cited by 70 publications

(77 citation statements)

References 26 publications

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“…Essa seleção foi feita antes mesmo que as amostras tivessem sido submetidas ao ELISA. A PCR foi realizada segundo Buling et al (2007). Para isso o DNA das amostras foi extraído, utilizando o QIAamp DNA Blood Mini Kit (Qiagen®), para determinar e quantificar os níveis parasitêmicos de B. bovis e B. bigemina.…”

Section: Methodsunclassified

Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará

et al. 2013

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Section: Methodsunclassified

Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará

et al. 2013

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“…A hot-start PCR (semi-nested) to detect B. bovis, B. bigemina based on aspartic proteinase babesipsin gene was developed and successfully applied (Martin et al, 2008;Awad et al, 2011). Multiplex PCR (Figueroa et al, 1993), real-time PCR (Buling et al, 2007) and multiplex LAMP (Iseki et al, 2007) are in process of development and validation for identification and characterization of B. bigemina.…”

Section: Pcrmentioning

confidence: 99%

Diagnostic approaches for tick-borne haemoparasitic diseases in livestock

D¹,

A²,

L³

2015

J. Vet. Med. Anim. Health

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Tick-borne diseases (TBDs) are a major economic constraint to livestock production affecting the productivity of livestock worldwide. Identification of these haemoprotozan and rickettsial infections is essential in understanding the epidemiology and it is important to distinguish between species and subspecies involved. Conventional techniques including serological and microscopic examinations do not always meet these requirements. Clinical diagnostic and surveillance tools, such as the complement fixation test (CFT), the indirect fluorescent antibody test (IFAT) and the enzyme linked immunosorbent assay (ELISA) have been successfully used over decades. In addition, DNA-based tests for diagnosis, differentiation and characterisation of different haemoparasites have been developed. Molecular diagnostic techniques, such as DNA hybridization and polymerase chain reaction (PCR), allow detection of parasites in blood, tissue or ticks with high levels of sensitivity, specificity and reliability. In addition, some techniques can identify multiple pathogens in the same samples. Furthermore, these techniques can also be exploited to identify unambiguous species and subspecies. Under the precondition that these tests are correctly designed and validated, they provide a powerful tool for epidemiology, with greater advantages of affordability and amenability to standardization. The implementation of these techniques for studying TBDs worldwide will be invaluable. Thus, the aim of this study is to put together the details of the techniques in the form of small review consultation of the practitioners and researchers.

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“…However, when BLASTn analysis was made, specifying that B. bigemina was the microorganism from which DNA were extracted, an 85.4% identity with B. bigemina strains reported throughout the world was found for both sequences -the ones coming from the vector and the bovine -in database GenBank, and an 86.9% identity with a B. bigemina strain (AY699276) (Buling et al 2007) for both sequences. However, the quality of the sequences, obtained as a result of direct sequentiation of the PCR amplification products, was not assertive in some nucleotidic sites, probably because of it being an amplified product of a multicopy gene (Cowman et al 1984, Adham et al 2009).…”

Section: Analysis Of Partial Sequences Obtained From 18s Rrna Genementioning

confidence: 99%

Assessing bovine babesiosis in Rhipicephalus (Boophilus) microplus ticks and 3 to 9-month-old cattle in the middle Magdalena region, Colombia

2014

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Babesia sp. is a protozoan hemoparasite that affects livestock worldwide. The Colombian Middle Magdalena is an enzootic region for babesiosis, but there is no previous research providing detail on its transmission cycle. This study aims to assess some Babesia sp. infection indicators in cattle and ticks from the area, by using direct microscopic and molecular techniques to detect the infection. In the cattle, 59.9% and 3.4 % positivity values for B. bigemina and mixed infection (B. bovis + B. bigemina) were found respectively. In ticks, the positivity of B. bigemina reached 79.2% and 9.4% for the mixed infection. The degree of infestation in the region was 3.2 ticks per bovine. There was positive correlation between tick control acaricide frequencies and infestation in bovines. This leads us to infer that control periodicity greater than 90 days, in stable zones, is an abiotic factor that benefits the acquisition of protective immunity in calves, the natural control of the infection and eventual disease absence. It is necessary to monitor the disease by applying new entomological and parasitological indicators showing the complexity of this phenomenon.

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A quantitative PCR assay for the detection and quantification of Babesia bovis and B. bigemina

Cited by 70 publications

References 26 publications

Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará

Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará

Diagnostic approaches for tick-borne haemoparasitic diseases in livestock

Assessing bovine babesiosis in Rhipicephalus (Boophilus) microplus ticks and 3 to 9-month-old cattle in the middle Magdalena region, Colombia

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