A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction

Maglio, Ornella; Costanzo, Salvatore; Cercola, Rosaria; Zambrano, Gerardo; Mauro, Marco Di; Battaglia, R.; Ferrini, G.; Nastri, Flavia; Pavone, Vincenzo; Lombardi, Angela

doi:10.3390/s17122747

Cited by 25 publications

(10 citation statements)

References 63 publications

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“…This follows from the negative mass balance by an amount exceeding the mass of the formed immune complexes. Obtained mass changes align significantly with literature results 37,38 …”

Section: Resultssupporting

confidence: 85%

Immunochemical biosensor for single virus particle detection based on molecular crowding polyelectrolyte system

Baldina

Nikolaev

Ivanov

et al. 2022

J of Applied Polymer Sci

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To improve the effectiveness of the viral infection diagnostic, we offer a new approach of immunochemical biosensors to determine single viral particles by specific antibodies. The antibodies are immobilized on the electrodes in a three-dimensional polymer matrix with several layers of polyelectrolytes on the screen-printed carbon electrode. Non-covalent immobilization of antibodies in successive layers of positively charged polyethyleneimine (PEI) and negatively charged polystyrene sulfonate (PSS) achieves the effect of macromolecular crowding. Such an immobilization approach promotes the preservation of the optimal conformation and antibody active center mobility for interaction with large virion particles. We established an electrochemical biosensor for tickborne encephalitis virus (TBEV) detection to demonstrate the method's applicability. Under the optimized architecture of the 3D-matrix, including a combination of two layers of a positively charged PEI with antibodies and the last layer of a negatively charged PSS, the assay is characterized by an extremely low limit of detection (LOD). This LOD could be as few as five viral particles in a sample volume of 5 μl, which is two orders of magnitude lower than conventional ELISA with the same reagents. The advantage of the biosensor is also a wide linear range of detection from 10 3 to 10 9 viral particles/ml. The proposed principle for determining virion particles is well suited to novel express diagnostics and Point-of-Care viral infections detection.

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“…This follows from the negative mass balance by an amount exceeding the mass of the formed immune complexes. Obtained mass changes align significantly with literature results 37,38 …”

Section: Resultssupporting

confidence: 85%

Immunochemical biosensor for single virus particle detection based on molecular crowding polyelectrolyte system

Baldina

Nikolaev

Ivanov

et al. 2022

J of Applied Polymer Sci

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“…Amino groups of proteins can be converted into thiols by the use of bi-functional linkers, containing both a thiol and a carboxylic group. Among sulfur-containing bi-functional linkers, LA has been widely applied for the preparation of a variety of stable conjugates, useful for different applications [ 61 , 62 , 63 , 64 , 65 , 66 ].…”

Section: Resultsmentioning

confidence: 99%

Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials

Zambrano

Ruggiero

Malafronte

et al. 2018

IJMS

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Many efforts are continuously devoted to the construction of hybrid biomaterials for specific applications, by immobilizing enzymes on different types of surfaces and/or nanomaterials. In addition, advances in computational, molecular and structural biology have led to a variety of strategies for designing and engineering artificial enzymes with defined catalytic properties. Here, we report the conjugation of an artificial heme enzyme (MIMO) with lipoic acid (LA) as a building block for the development of gold-based biomaterials. We show that the artificial MIMO@LA can be successfully conjugated to gold nanoparticles or immobilized onto gold electrode surfaces, displaying quasi-reversible redox properties and peroxidase activity. The results of this work open interesting perspectives toward the development of new totally-synthetic catalytic biomaterials for application in biotechnology and biomedicine, expanding the range of the biomolecular component aside from traditional native enzymes.

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“…According to the result performance, it has the approximate sensitivity of 6.5 Hz/mL to sense the odor of ethanol. Another research paper shows that the frequency change curve resulted by specific odor, fragrance, and gas in the range of hundreds herzt [31]. Our system offers higher operating resonant frequency.…”

Section: Results and Analysismentioning

confidence: 85%

Design of Electronic Nose System Using Gas Chromatography Principle and Surface Acoustic Wave Sensor

et al. 2018

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Most gases are odorless, colorless and also hazard to be sensed by the human olfactory system. Hence, an electronic nose system is required for the gas classification process. This study presents the design of electronic nose system using a combination of Gas Chromatography Column and a Surface Acoustic Wave (SAW). The Gas Chromatography Column is a technique based on the compound partition at a certain temperature. Whereas, the SAW sensor works based on the resonant frequency change. In this study, gas samples including methanol, acetonitrile, and benzene are used for system performance measurement. Each gas sample generates a specific acoustic signal data in the form of a frequency change recorded by the SAW sensor. Then, the acoustic signal data is analyzed to obtain the acoustic features, i.e. the peak amplitude, the negative slope, the positive slope, and the length. The Support Vector Machine (SVM) method using the acoustic feature as its input parameters are applied to classify the gas sample. Radial Basis Function is used to build the optimal hyperplane model which devided into two processes i.e., the training process and the external validation process. According to the result performance, the training process has the accuracy of 98.7% and the external validation process has the accuracy of 93.3%. Our electronic nose system has the average sensitivity of 51.43 Hz/mL to sense the gas samples.

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A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction

Cited by 25 publications

References 63 publications

Immunochemical biosensor for single virus particle detection based on molecular crowding polyelectrolyte system

Immunochemical biosensor for single virus particle detection based on molecular crowding polyelectrolyte system

Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials

Design of Electronic Nose System Using Gas Chromatography Principle and Surface Acoustic Wave Sensor

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