A rabbit model to tissue engineer the bladder

Coadministration of Platelet-Derived Growth Factor-BB and Vascular Endothelial Growth Factor with Bladder Acellular Matrix Enhances Smooth Muscle Regeneration and Vascularization for Bladder Augmentation in a Rabbit Model

“…29 Therefore, urothelium formation could be well accomplished within a short time of 2-4 weeks. [30][31][32] In our study, multilayered urothelium formed well in both the marginal zone and the central zone of the two groups at 1 month, which further supports the results of previous studies. ] in the experimental rabbit neobladder were higher than those in the control group at 1, 2, and 3 months after surgery (*p < 0.05).…”

Cistoplastia experimental em coelhos (Oryctolagus cuniculus) com peritônio bovino conservado em glicerol a 98%

“…Foram colocados dois pontos de reparo na extremidade cranial e dois na extremidade caudal do plano a ser incisado, para auxiliar e facilitar a manipulação do órgão (STONE, 1996) e, de acordo com NUININGA et al (2004), da face ventral da vesícula urinária, após incisão em estocada, retirou-se um fragmento de 1,5 x 1,5cm, o qual se substituiu por retalho de peritônio bovino, de mesma dimensão (Figura 1A). Previamente ao seu implante, o retalho foi colocado em recipiente estéril, imerso em aproximadamente 20mL da solução fisiológica de NaCl 0,9% acrescida de solução contendo ampicilina sódica (100mg mL -1 ), por 10 minutos (DALECK et al, 1987;DALECK et al, 1988).…”

Cell colonization in degradable 3D porous matrices

“…SIS promotes cell migration of numerous cell types and has been tested for regeneration of diverse tissues including large vascular grafts, 15 venous valves and leaflets, [16][17][18] skin, 19 tendons 20 and wound dressing. 21 For urinary tract reconstruction, SIS has been used for bladder augmentation, [22][23][24] for ureter 25 and urethra 22,26 replacement and to promote regeneration of transitional epithelium, smooth muscle and peripheral nerves with no evidence of immunological rejection. 27 Long-term studies show that SIS grafts can be remolded and replaced by the host and such regenerated tissues become histologically indistinguishable from native tissues.…”