A radioimmunoassay for the R-type binders of cobalamin

Morelli, T. A.; Begley, James A.; Hall, Charles A.

doi:10.1016/0009-8981(77)90242-x

Cited by 13 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was kept at -20°C until use. Total serum TC II and R binders were measured by radioimmunoassay (14,15). Total serum Cbl and its division between TC II and R binders were measured by an isotope dilution assay (16).…”

Section: Methodsmentioning

confidence: 99%

“…Total serum Cbl and its division between TC II and R binders were measured by an isotope dilution assay (16). The TC II-Cbl was separated before assay by fractionation with 1.96 M (NH4)2SO4 (15,16). The unsaturated Cbl binding capacity was determined by saturating the apoprotein (apo) TC II and serum R with radioactive Cbl and then separating the TC II from the serum R binders by both Sephadex G-200 (Pharmacia Fine Chemicals, Inc., Piscataway, N.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Megaloblastic anemia as a result of an abnormal transcobalamin II (Cardeza).

Haurani¹,

Hall²,

Rubin³

1979

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Megaloblastic anemia as a result of an abnormal transcobalamin II (Cardeza).

Haurani¹,

Hall²,

Rubin³

1979

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…The sera were obtained either directly from the appropriate species or purchased and stored at -20" until use. The CN [57Co]Cb 1, nonradioactive Cbl, rabbit antibody against pure human TC 11, and cultural cells have been described previously (2,7,8,(12)(13)(14)(15).…”

mentioning

confidence: 99%

“…I . RIA of the TC I1 from eight species by an assay for human TC I1 based on rabbit antibody against pure human TC I1(12). The amount of material applied was adjusted to contain 100 pg of TC 11.…”

mentioning

confidence: 99%

Species Specificity in the Immunologic Reactions and Biological Functions of Transcobalamin II

Haus

Green

Hall

1979

Experimental Biology and Medicine

View full text Add to dashboard Cite

Genetic patterns of transcobalamin II and the relationships with congenital defects

Fràter‐Schröder

1983

Mol Cell Biochem

View full text Add to dashboard Cite

The vitamin B12-binding protein, transcobalamin II, is a trace component of plasma with a rapid turnover. This protein is essential for absorption, transport, cellular uptake and for recycling of vitamin B12 (cobalamin). Congenital transcobalamin II deficiency, an inborn error of metabolism is inherited as a recessive trait. The homozygous form of the deficiency is accompanied by severe clinical, hematological and immunological disturbances in the first months of life. Analytical, genetic, biochemical and clinical aspects of transcobalamin II in man and in vertebrates have been reviewed here. A genetic polymorphism for the protein has been found in man, rabbits and mice. Family studies revealed that the genetic patterns in man are determined by four polymorphic and several rare alleles. This genetic variability has been applied in paternity testing and in population studies. Transcobalamin II typing in families of patients with the inherited functional deficiency has led to identification of various deficient alleles in heterozygous carriers of the defects. Applying transcobalamin II typing after bone marrow transplantation demonstrated that this protein originates partly in the bone marrow. Subsequent investigations in cell culture have shown that human skin fibroblasts and cultured bone marrow synthesize and secret isotypes of a transport protein corresponding to the genetic isotypes observed in plasma. Comparison of transcobalamin II types in umbilical cord serum with the maternal types, has proven that the transcobalamin II activity in the cord serum is derived from the fetus. This finding will be of crucial importance in the early diagnosis of the deficiency syndrome.

show abstract

A radioimmunoassay for the R-type binders of cobalamin

Cited by 13 publications

References 11 publications

Megaloblastic anemia as a result of an abnormal transcobalamin II (Cardeza).

Megaloblastic anemia as a result of an abnormal transcobalamin II (Cardeza).

Species Specificity in the Immunologic Reactions and Biological Functions of Transcobalamin II

Genetic patterns of transcobalamin II and the relationships with congenital defects

Contact Info

Product

Resources

About