A randomised controlled comparison of aspiration and non-aspiration fine-needle techniques for obtaining ultrasound-guided cytological samples from canine livers

Fleming, Kathryn L.; Howells, Esme; Villiers, Elizabeth; Maddox, Thomas W.

doi:10.1016/j.tvjl.2019.105372

Cited by 10 publications

(17 citation statements)

References 27 publications

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“…Ultrasound-guided FNNA sampling of the canine and feline spleen was shown previously to provide smears of higher cellularity and less blood (but similar cell preservation) compared to samples obtained by FNA (LeBlanc et al 2009). More recently, FNNA was found to be superior to FNA for sampling liver in the dog, resulting in higher rates of diagnostic samples that were less hemodiluted and had higher cellularity and better cell preservation (Fleming et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Cytologic smears should be of high cellularity, with good cell preservation and minimal blood contamination, to accurately reflect the underlying LN pathology (Cowell et al 2003, Amores-Fuster et al 2015. Negative pressure from aspiration can contribute to cell lysis and blood contamination that could compromise cytologic diagnosis (Fleming et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…In humans, this has been shown for sampling palpably or ultrasonographically accessible LNs, thyroid or mammary nodules, and other tissues (Savage et al 1995, De Carvalho et al 2009, Romitelli et al 2009, Sajeev & Siddaraju 2009, Jahromi et al 2015, Misra et al 2015, Song et al 2015. In companion animals, ultrasound-guided FNNA sampling was shown to provide smears of higher cytologic and diagnostic quality compared to FNA sampling in the canine and feline spleen and the canine liver (LeBlanc et al 2009, Fleming et al 2019. However, to the authors' knowledge, the effect of aspiration on cytologic specimens obtained from canine LNs has yet to be assessed.…”

Section: Introductionmentioning

confidence: 99%

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A comparison of cytologic quality in fine‐needle specimens obtained with and without aspiration from superficial lymph nodes in the dog

Karakitsou

Christopher

Meletis

et al. 2021

J of Small Animal Practice

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Objectives: To assess the impact of aspiration on cytologic quality of fine-needle specimens obtained from lymph nodes of dogs and to compare the level of agreement of the cytologic diagnosis between the two sampling techniques.Materials and MethOds: Fifty-three client-owned dogs were prospectively enrolled. Client-owned dogs were prospectively enrolled in the study if cytologic examination of lymph nodes was indicated in the course of their clinical diagnostic work-up. In each dog, two superficial, palpably accessible lymph nodes were sampled: one by fine-needle aspiration and the other by fine-needle non-aspiration, using a 21-Gauge needle with or without a 5-mL syringe. Cytologic quality was assessed in duplicate smears assessed by two observers who rated cellularity, blood contamination, thickness, cell preservation and cytoplasmic fragmentation using a predefined scoring system. results: Fifty-three client-owned dogs were included in the study. No significant difference was found in rating scores between the two sampling techniques for any of the cytologic quality parameters assessed. Cohen's kappa coefficient was 0.84 (95% confidence interval 0.68-1.00), indicating diagnostic agreement between the sampling techniques.clinical significance: In this study, both fine-needle aspiration and fine-needle non-aspiration techniques yielded lymph node specimens of comparable cytologic quality and with acceptable agreement in cytologic diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A comparison of cytologic quality in fine‐needle specimens obtained with and without aspiration from superficial lymph nodes in the dog

Karakitsou

Christopher

Meletis

et al. 2021

J of Small Animal Practice

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“…19,20 A study investigating the two techniques for the sampling of canine livers concluded that FNNAC was more likely to produce a diagnostic sample compared to FNAC. 25 In addition, a study analysing splenic sampling in feline and canine patients found samples obtained by FNNAC had significantly higher cellularity, less blood and similar cell morphology compared with samples obtained by FNAC. 26 The fact that our results are contradictory to these findings is likely due to the differences between lymph nodes and these organs in terms of morphology and vascularity.…”

Section: Discussionmentioning

confidence: 99%

A comparison of cytological quality between fine‐needle aspiration and non‐aspiration techniques for obtaining ultrasound‐guided samples from canine and feline lymph nodes

Whitlock

Taeymans²,

Monti³

2021

Veterinary Record

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Background: In small animal medicine, ultrasound‐guided fine‐needle lymph node sampling plays a pivotal role in the diagnostic investigation of a range of pathologies including the staging of neoplastic disease. Traditionally fine needle aspiration cytology (FNAC) has been employed to produce samples, but fine needle non‐aspiration cytology (FNNAC) has been suggested to generate superior sample quality and diagnosticity. Methods: In a randomised control trial, 104 canine and feline lymph nodes were each sampled by both techniques. The cytological samples were then submitted to pathologists who were blinded to the technique used to generate each sample. They determined if the sample was diagnostic or non‐diagnostic and graded the sample in terms of the degree of cellularity, cellular preservation and haemodilution. Results: It was found that lymph node samples obtained using the FNAC technique were more likely to be diagnostic (p = 0.043) than samples obtained using the FNNAC technique. In addition, FNAC samples had significantly higher cellularity than FNNAC counterparts (P = 0.043). No significant difference in cell preservation or haemodilution was found between samples from the FNAC and FNNAC groups. Conclusion: In this study, FNAC was superior to non‐aspiration cytology for the sampling of canine and feline lymph nodes as it generated a higher number of diagnostic samples with greater cellularity.

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“…In the patients with liver metastasis from lymphomas, there was 100% agreement between the left and right liver samples, with an intermediate to high CAS. It is well known that hemolymphatic lymphoma metastasis causes diffuse infiltration of the neoplastic cells in the liver parenchyma [ 19 ], and lymphoma can be reliably diagnosed cytologically [ 24 , 25 ]. In addition, hepatomegaly caused by neoplastic cell infiltration may also increase accessibility when performing ultrasound-guided fine-needle liver sampling for cytology.…”

Section: Discussionmentioning

confidence: 99%

Comparison of fine-needle cytologic diagnosis between the left and right liver lobes of dogs and cats with diffuse liver disease

et al. 2021

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Background and Aim: Ultrasound-guided fine-needle sample collection for cytology with manual restraint is frequently used for the primary assessment of diffuse liver disease in veterinary patients in Thailand. For better diagnosis, repeated collection of samples ensures the collection of adequate, representative samples, which increase diagnostic accuracy. However, in those that are unable to receive general anesthesia, it is difficult to collect the samples from several liver locations in manually restrained dogs and cats. The study aimed to compare the cytologic diagnosis of the ultrasound-guided fine-needle non-aspiration technique between the left and right liver lobes in dogs and cats with neoplastic and non-neoplastic diffuse liver disease. Materials and Methods: This prospective study included 25 client-owned dogs and cats with diffuse liver diseases. Two liver samples were randomly collected from the left and right liver lobes under ultrasound guidance for cytologic examination. All slides were subsequently examined blindly by experienced pathologists for cytologic analysis with cytologic agreement scores (CASs). Results: Among all 50 samples obtained from ultrasound-guided fine-needle sample collection of the left and right liver, 78% were diagnostic and 22% were non-diagnostic. In the diagnostic group, 73.3% of fine-needle samples had concordant results between the left and right liver, which exhibited 100% cytologic agreement in lymphoma and 63.6% in non-neoplastic groups. Samples collected from the left liver had slightly higher CAS and higher cytologic quality than had those from the right liver lobe (p=0.053). Conclusion: The location and number of sample collections did not have a significant difference in the cytologic diagnosis of diffuse liver disease, especially in patients with lymphoma. For manually restrained patients, one time ultrasound-guided non-aspiration cytology procedure from the left liver lobe not only decreased restraint duration and minimized tissue trauma but also allowed for an adequate cytologic diagnosis in diffuse liver disease compared to multiple collections.

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A randomised controlled comparison of aspiration and non-aspiration fine-needle techniques for obtaining ultrasound-guided cytological samples from canine livers

Cited by 10 publications

References 27 publications

A comparison of cytologic quality in fine‐needle specimens obtained with and without aspiration from superficial lymph nodes in the dog

A comparison of cytologic quality in fine‐needle specimens obtained with and without aspiration from superficial lymph nodes in the dog

A comparison of cytological quality between fine‐needle aspiration and non‐aspiration techniques for obtaining ultrasound‐guided samples from canine and feline lymph nodes

Comparison of fine-needle cytologic diagnosis between the left and right liver lobes of dogs and cats with diffuse liver disease

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