2015
DOI: 10.1371/journal.pone.0132441
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A Rapid and Economical Method for Efficient DNA Extraction from Diverse Soils Suitable for Metagenomic Applications

Abstract: A rapid, cost effective method of metagenomic DNA extraction from soil is a useful tool for environmental microbiology. The present work describes an improved method of DNA extraction namely “powdered glass method” from diverse soils. The method involves the use of sterile glass powder for cell lysis followed by addition of 1% powdered activated charcoal (PAC) as purifying agent to remove humic substances. The method yielded substantial DNA (5.87 ± 0.04 μg/g of soil) with high purity (A260/280: 1.76 ± 0.05) an… Show more

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Cited by 49 publications
(33 citation statements)
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References 40 publications
(53 reference statements)
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“…As a final added advantage, the overall processing time for the ISB method was shorter (1:30 h) than 5 to 7 h of the earlier reported methods (Sagar et al 2014). The commercial kit (CK) took the least time for DNA extraction (Table 3); however, the processing cost of 1 g of soil or tissue for a single reaction is about US$7 which is quite high if large numbers of samples are needed to be processed (Devi et al 2015).…”
Section: Discussionmentioning
confidence: 95%
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“…As a final added advantage, the overall processing time for the ISB method was shorter (1:30 h) than 5 to 7 h of the earlier reported methods (Sagar et al 2014). The commercial kit (CK) took the least time for DNA extraction (Table 3); however, the processing cost of 1 g of soil or tissue for a single reaction is about US$7 which is quite high if large numbers of samples are needed to be processed (Devi et al 2015).…”
Section: Discussionmentioning
confidence: 95%
“…6 and 7). A potential reason for low performance of cell disruption in soil samples by bead beating of power soil kit may be due to the increase of viscosity and the presence of a high concentration of insoluble materials during the beating process (Devi et al 2015). High losses of input DNA during the application of commercial kits have been reported (Roossinck et al 2010;Vo and Jedlicka 2014;Sadeghi et al 2013).…”
Section: Discussionmentioning
confidence: 99%
“…Successful DNA isolation would be one that could extract the high yield consistently across the samples from a large number of individuals with high quantity, purity and the less time period. Different kinds of methods are available for gDNA extraction based on chemical and mechanical lysis of cells (Devi et al, 2015). There are an ambiguity and problems in choosing the best methods that not only are able to recover a high amount of nucleic acids but also give a higher yield of amplifiable copies.…”
Section: Discussionmentioning
confidence: 99%
“…The concentration and purity of genomic DNA were determined by measuring the A 260/ A 280 ratio. Genomic DNA was extracted from the soil samples as previously described by Devi .…”
Section: Methodsmentioning
confidence: 99%