A Rapid and Sensitive Liquid Chromatography- Mass Spectrometry/Mass Spectrometry Method for Estimation of Pioglitazone, Keto Pioglitazone and Hydroxy Pioglitazone in Human Plasma

Abstract

Objective: The main objective of the work was to develop a straightforward, fast and selective liquid chromatography/tandem mass spectrometry (LC-MS/MS) assay for determination of pioglitazone (PG), keto pioglitazone (KPG), and hydroxy pioglitazone (HPG) in human plasma and to validate as per recent guidelines. Methods:Analyte and the internal standard (IS) were extracted from plasma through liquid-liquid extraction and chromatographed on a Xterra RP18, 100×4.6, 5 µ column using methanol: acetonitrile mixture and 10 mM Ammonium formate buffer (70:30, v/v) as the mobile phase at a flow rate of 0.7 mL/min. The API-3200 Q Trap LC-MS/MS instrument in multiple reaction monitoring mode was used for detection. Diphenhydramine was utilized as IS. Results:The linearity was established in the concentration range of 20.15-1007.58 ng/mL for PG, 20.35-1017.58 ng/mL for KPG, and 19.68-491.22 ng/mL for HPG in human plasma. All the validation parameters were well within the acceptance limits. Conclusion:A new simple LC-MS/MS method was developed for the determination of PG, KPG, and HPG in human plasma. This method can be easily applied for the estimation of pharmacokinetic parameters of PG, KPG, and HPG.

ABSOLUTE AND RELATIVE BIOAVAILABILITY STUDY FOR THE NEWLY DEVELOPED NASAL NANOEMULSION IN SITU GEL OF ONDANSETRON HCl IN COMPARISON TO CONVENTIONALLY PREPARED IN SITU GEL AND INTRAVENOUS DOSAGES FORMS

“…Intravenous administration of 700 μl ZofranP ® P injection (2 mg/ml) was given to the rabbits. Blood samples (2 ml) were withdrawn from the marginal ear vein at zero time (first minute after administration) and 0.25, 0.5, 1.0, 2.0, 3.0, 5.5, 7.5, 10 and 24 h. Blood samples were centrifuged to collect plasma and collected in tubes containing EDTA and immediately frozen at-20 °C until analysis [12].…”

Development and Validation of Sensitive Lc-Esi-MS/MS Method for the Simultaneous Estimation of Dapagliflozin and Saxagliptin in Human Plasma

“…Potential nosiness between analyte and endogenous matrix constituents was examined by the analysis of 6 lots blank plasma from different source. From each lot, a double blank and a LLOQ sample were prepared, infused and analysed [16,19]. To determine potential nosiness between IS and analytes, blank sample spiked with analytes separately (at the upper limit of quantification) and IS were infused and estimated.…”

“…Matrix Factor (MF) of analytes in plasma were processed at HQC and LQC levels after extraction in six different blank matrix batches. Simultaneously six duplicates of equivalent neat quality control samples were prepared and estimated [18][19][20][21][22]. Assess the MF for analytes and IS in each batch by the application of formula:…”

Electrochemical Studies of Anti‐HIV Drug Emtricitabine: Oxidative Determination and Improved Antimicrobial Activity

“…Moreover, by using LC‐MS/MS determination of EMT, tenofovir, efavirenz, lopinavir and ritonavir concentration and THF/water solvent system in HIV infected plasma was also studied . The stability of EMT, Tenofovir, Cobicistat and Elvitegravir from their degradation product studied by UPLC analytical method by Rao and coworkers . Eventhough, all these aforementioned approaches are well established but are accompanying with certain limitations like‐high cost, need special trained person to handle, having low sensitivity and poor selectivity etc.…”