A rapid and simple HPLC method for the determination of curcumin in rat plasma: assay development, validation and application to a pharmacokinetic study of curcumin liposome

Abstract

This paper describes a sensitive, specific and rapid high-performance liquid chromatography (HPLC) method for the determination of curcumin in rat plasma. After a simple step of protein precipitation in 96-well format using acetonitrile containing the internal standard (IS), emodin, plasma samples were analyzed by reverse-phase HPLC. Curcumin and the IS emodin were separated on a Diamonsil C(18) analytical column (4.6 x 100 mm, 5 microm) using acetonitrile-5% acetic acid (75:25, v/v) as mobile phase at a flow rate of 1.0 mL/min. The method was sensitive with a lower limit of quantitation of 1 ng/mL, with good linearity (r(2) >or= 0.999) over the linear range 1-500 ng/mL. All the validation data, such as accuracy and precision, were within the required limits. A run time of 3.0 min for each sample made high-throughput bioanalysis possible. The assay method was successfully applied to the study of the pharmacokinetics of curcumin liposome in rats.

Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

“…The 71 increased breakdown of muscle proteins, in particular the myofibrillar proteins actin and myosin, is a common metabolic consequence of the above mentioned diseases, resulting in muscle wasting, weakness and fatigue (Poylin et al, 2008;Li et al, 2009). Among muscle wasting-related transcription factors, NF-kB (nuclear factor-kappaB) has been lately of study interest (Aggarwal and Sung, 2009).…”

Development and validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles

“…2) is a natural compound extracted from Curcuma Longa L. rhizome, which has showed numerous therapeutic properties, among them are antiparkinson, antineoplastic, anti-inflammatory, antioxidant, antibacterial, antifungal, and antiviral (Joshi, 2010;Zandi et al, 2010;Yallapu et al, 2012;Mandal, 2017).…”

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“…During literature survey we came across a HPLC method for the estimation of curcumin in rat plasma 11 . This method employed a mobile phase consisting of acetonitrile-5% acetic acid.…”