A Rapid and Specific Assay for the Detection of MERS-CoV

Huang, Pei; Wang, Hualei; Cao, Zongjie; Jin, Hongli; Chi, Hang; Zhao, Jincun; Yu, Bin; Yan, Feihu; Hu, Xingxing; Wu, Fangfang; Jiao, Cuicui; Hou, Peng; Xu, Shengnan; Zhao, Yongkun; Feng, Na; Wang, Jianzhong; Sun, Wenzhao; Wang, Tiecheng; Gao, Yuwei; Wang, Cuiling; Xia, Xianzhu

doi:10.3389/fmicb.2018.01101

Cited by 152 publications

(132 citation statements)

References 26 publications

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“…Coronavirus may cause respiratory and neurological diseases 52 . So far, six human coronaviruses (HCoVs) have been identified, including HCoV‐229E, HCoV‐HKU1, HCoV‐OC43, HCoV‐NL63, severe acute respiratory syndrome coronavirus (SARS‐CoV), and Middle East respiratory syndrome coronavirus (MERS‐CoV) 53 . MERS‐CoV is a human coronavirus first reported in June 2012 that can cause human respiratory diseases 52 .…”

Section: Coronavirus and Its Diagnostic Approachesmentioning

confidence: 99%

Recent advances in the detection of respiratory virus infection in humans

Zhang

Wang

Deng

et al. 2020

Journal of Medical Virology

445

363

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Respiratory tract viral infection caused by viruses or bacteria isone of the most common diseases in human worldwide, while those caused by emerging viruses, such as the novel coronavirus, 2019-nCoV that caused the pneumonia outbreak in Wuhan, China most recently, have posed great threats to global public health. Identification of the causative viral pathogens of respiratory tract viral infections is important to select an appropriate treatment, save people's lives, stop the epidemics, and avoid unnecessary use of antibiotics. Conventional diagnostic tests, such as the assays for rapid detection of antiviral antibodies or viral antigens, are widely used in many clinical laboratories. With the development of modern technologies, new diagnostic strategies, including multiplex nucleic acid amplification and microarray-based assays, are emerging. This review summarizes currently available and novel emerging diagnostic methods for the detection of common respiratory viruses, such as influenza virus, human respiratory syncytial virus, coronavirus, human adenovirus, and human rhinovirus. Multiplex assays for simultaneous detection of multiple respiratory viruses are also described. It is anticipated that such data will assist researchers and clinicians to develop appropriate diagnostic strategies for timely and effective detection of respiratory virus infections. K E Y W O R D S adenovirus, coronavirus, diagnostic methods, influenza virus, respiratory syncytial virus, respiratory viral infection, rhinovirus

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Section: Coronavirus and Its Diagnostic Approachesmentioning

confidence: 99%

Recent advances in the detection of respiratory virus infection in humans

Zhang

Wang

Deng

et al. 2020

Journal of Medical Virology

445

363

View full text Add to dashboard Cite

show abstract

“…84 Several diagnostic tests are under development to accelerate turnaround times from sample analysis to result. [85][86][87] A 2018 rapid and specific assay for the detection of MERS-CoV combines the reverse transcription loop-mediated isothermal amplification technique and a vertical flow visualisation strip (RT-LAMP-VF) to detect the nucleocapsid gene of MERS-CoV. 86 The RT-LAMP-VF assay provides detection of MERS-CoV within 35 min and is easier to perform than the WHO-recommended rRT-PCR method.…”

Section: Laboratory Testingmentioning

confidence: 99%

“…[85][86][87] A 2018 rapid and specific assay for the detection of MERS-CoV combines the reverse transcription loop-mediated isothermal amplification technique and a vertical flow visualisation strip (RT-LAMP-VF) to detect the nucleocapsid gene of MERS-CoV. 86 The RT-LAMP-VF assay provides detection of MERS-CoV within 35 min and is easier to perform than the WHO-recommended rRT-PCR method. 86 Several serological assays are available for detection of MERS-CoV: ELISA, recombinant spike immunofluorescent assay, and spike pseudoparticle neutralisation and microneutralisation assay.…”

Section: Laboratory Testingmentioning

confidence: 99%

“…86 The RT-LAMP-VF assay provides detection of MERS-CoV within 35 min and is easier to perform than the WHO-recommended rRT-PCR method. 86 Several serological assays are available for detection of MERS-CoV: ELISA, recombinant spike immunofluorescent assay, and spike pseudoparticle neutralisation and microneutralisation assay. [87][88][89][90] A case confirmed by serology requires evidence of seroconversion in two samples, ideally taken at least 14 days apart, by a screening (eg, ELISA, immunofluorescence assay) and a neutralisation assay.…”

Section: Laboratory Testingmentioning

confidence: 99%

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Middle East respiratory syndrome

et al. 2020

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The Middle East respiratory syndrome coronavirus (MERS-CoV) is a lethal zoonotic pathogen that was first identified in humans in Saudi Arabia and Jordan in 2012. Intermittent sporadic cases, community clusters, and nosocomial outbreaks of MERS-CoV continue to occur. Between April 2012 and December 2019, 2499 laboratory-confirmed cases of MERS-CoV infection, including 858 deaths (34·3% mortality) were reported from 27 countries to WHO, the majority of which were reported by Saudi Arabia (2106 cases, 780 deaths). Large outbreaks of human-to-human transmission have occurred, the largest in Riyadh and Jeddah in 2014 and in South Korea in 2015. MERS-CoV remains a high-threat pathogen identified by WHO as a priority pathogen because it causes severe disease that has a high mortality rate, epidemic potential, and no medical countermeasures. This Seminar provides an update on the current knowledge and perspectives on MERS epidemiology, virology, mode of transmission, pathogenesis, diagnosis, clinical features, management, infection control, development of new therapeutics and vaccines, and highlights unanswered questions and priorities for research, improved management, and prevention.

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“…Due to the growing demands of on-site diagnosis in medicine, the realization of point-of-care (POC) PCR strategies has garnered much attention (Petralia and Conoci, 2017). Thus, PCR has been used for diagnosing many infectious diseases, such as HIV (Mackay et al, 2002), the coronavirus genus (Drosten et al, 2003;Huang et al, 2018), Epstein-Barr virus (Yamamoto et al, 1995), and eubacterial (Hryniewiecki et al, 2002;McCabe et al, 1995) and Escherichia coli infections (Holland et al, 2000;Kim et al, 2018). Several million copies of DNA can be generated from a single molecule through only few cycles of PCR.…”

Section: Introductionmentioning

confidence: 99%

Thermally stable and uniform DNA amplification with picosecond laser ablated graphene rapid thermal cycling device

Chen

Chang

et al. 2019

Biosensors and Bioelectronics

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Rapid thermal cycling (RTC) in an on-chip device can perform DNA amplification in vitro through precise thermal control at each step of the polymerase chain reaction (PCR). This study reports a straightforward fabrication technique for patterning an on-chip graphene-based device with hole arrays, in which the mechanism of surface structures can achieve stable and uniform thermal control for the amplification of DNA fragments. A thin-film based PCR device was fabricated using picosecond laser (PS-laser) ablation of the multilayer graphene (MLG). Under the optimal fluence of 4.72 J/cm 2 with a pulse overlap of 66%, the MLG can be patterned with arrays of 250 μm 2 hole surface structures. A 354-bp DNA fragment of VP1, an effective marker for diagnosing the BK virus, was amplified on an on-chip device in less than 60 min. A thin-film electrode with the aforementioned MLG as the heater was demonstrated to significantly enhance temperature stability for each stage of the thermal cycle. The temperature control of the heater was performed by means of a developed programmable PCR apparatus. Our results demonstrated that the proposed integration of a graphene-based device and a laser-pulse ablation process to form a thin-film PCR device has cost benefits in a small-volume reagent and holds great promise for practical medical use of DNA amplification.

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A Rapid and Specific Assay for the Detection of MERS-CoV

Cited by 152 publications

References 26 publications

Recent advances in the detection of respiratory virus infection in humans

Recent advances in the detection of respiratory virus infection in humans

Middle East respiratory syndrome

Thermally stable and uniform DNA amplification with picosecond laser ablated graphene rapid thermal cycling device

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