A rapid electrophoretic procedure for the detection of SDS-released oncorna-viral RNA using polyacrylamide-agarose gels

Harewood, Ken R.; Wolff, John S.

doi:10.1016/0003-2697(73)90146-2

Cited by 15 publications

(11 citation statements)

References 5 publications

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“…SLS properties, such as low superficial tension and lubricant ability, certainly help the flushing action and cleaning of the root canal system (18). Though, it does not have antimicrobial action (13) and has some level of cytotoxicity, since it solubilizes proteins (19). In the present study, SLS was cytotoxic at all concentrations, and thus this solution does not fulfill the requirements for an acceptable endodontic irrigant.…”

Section: Discussionmentioning

confidence: 68%

Cytotoxicity of endodontic irrigants containing calcium hydroxide and sodium lauryl sulphate on fibroblasts derived from mouse L929 cell line

2009

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The aim of this study was to evaluate the cytotoxicity of root canal irrigating solutions containing calcium hydroxide and sodium lauryl sulphate on fibroblasts derived from L929 cell line. Saturated calcium hydroxide aqueous solution (CH), sodium lauryl sulphate (SLS) and SLS associated with calcium hydroxide (HCT20) were diluted with sterile distilled water at 50%, 20%, 10% and 5% concentrations. Minimum essential medium (MEM) served as the control group. The cytotoxicity of the solutions was evaluated on L929 mouse fibroblast cell line, at 4 and 24 h of contact time by the 51Cr radiotracer method. Data were compared and statistical inferences were made with the chi-square test. In all analysis, significance level was set at 5%. CH and HCT20 showed toxicity at 50% concentration, while at concentrations lower than 50% these solutions showed cell tolerance. SLS was cytotoxic at all concentrations. In conclusion, the association of calcium hydroxide and SLS (HCT20) combines the beneficial properties of these solutions and was not harmful to the fibroblast cell line, seeming to be a suitable endodontic irrigating solution.

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Section: Discussionmentioning

confidence: 68%

Cytotoxicity of endodontic irrigants containing calcium hydroxide and sodium lauryl sulphate on fibroblasts derived from mouse L929 cell line

2009

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“…The crystals were next placed into an UV/Ozone chamber (Bioforce Nanosciences, Ames, IA) for 10 min [37]. The crystals were submerged in 2 – 4% (v/v) sodium dodecyl sulfate in nanopure water for 30 min to remove any biological contaminants [38, 39], followed by rinses with water and then ethanol, drying with a nitrogen stream, and a final cycle in the UV/Ozone cleaner just prior to use.…”

Section: Methodsmentioning

confidence: 99%

Supported Phospholipid Bilayer Interaction with Components Found in Typical Room-Temperature Ionic Liquids – a QCM-D and AFM Study

Evans

2008

IJMS

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Quartz crystal microbalance with dissipation (QCM-D) monitoring and atomic force microscopy (AFM) were combined to evaluate the defects created by an ionic liquid anion and a cation in a supported phospholipid bilayer composed of zwitterionic lipids on a silica surface. The cation 1-octyl-3-methyl imidazolium (OMIM + ) was shown to remove lipids from the bilayer, increase the roughness to approximately 2.8 nm (~0.2 for stable supported bilayer) and possibly redeposit lipids with entrapped water. The anion bis(trifluoromethylsulfonyl)imide (Tf 2 N -) was found to leave distinct defects within the bilayer that had large pore-like interiors which left the surrounding bilayer intact. However, the ionic liquid 1-butyl-1-methyl pyrrolidinium bis(trifluoromethylsulfonyl)imide (BMPTf 2 N) formed a film over the supported bilayer. This work demonstrates, for the first time, the direct effects common components of ionic liquids have on a supported phospholipids bilayer.

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“…The purified virus suspended in TEN buffer was deproteinized by the addition of SDS to 0.5 per cent (v/v) and pronase (500 mg/ml), followed by incubation at 37 ° C for 45 to 60 minutes (5). In some experiments the purified labeled virus was disrupted directly with SDS (1 per cent final concentration) and analyzed in polyaerylamide-agarose gels (6). The RNA fractions were recovered by precipitation with ethanol and sodium acetate as described (5).…”

mentioning

confidence: 99%

“…Polyacrytamide-agarose gel electrophoretic analysis of viral RNAs was performed according to the method of HAREWOOD and WOLFF (6).…”

mentioning

confidence: 99%

The RNA of the human syncytium-forming (foamy) virus

Loh

Matsuura

1981

Archives of Virology

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Human syncytium-forming (foamy) virus was labeled with 3H-uridine and banded isopycnically in sucrose gradients (buoyant density = 1.16 to 1.18 g/cm3). Viral RNA extracted from the banded virus was analyzed either by rate zonal separation in sucrose gradients or by polyacrylamide-agarose gel electrophoresis. The results indicated that purified HSFV contains a 60S RNA component plus several smaller molecular weight RNA components. On dissociation with heat, smaller RNA structures were released from the 60S component. These results indicate that the genome of HSFV, like the other members of the Retroviridae family, is composed of an aggregate of several RNA species.

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A rapid electrophoretic procedure for the detection of SDS-released oncorna-viral RNA using polyacrylamide-agarose gels

Cited by 15 publications

References 5 publications

Cytotoxicity of endodontic irrigants containing calcium hydroxide and sodium lauryl sulphate on fibroblasts derived from mouse L929 cell line

Cytotoxicity of endodontic irrigants containing calcium hydroxide and sodium lauryl sulphate on fibroblasts derived from mouse L929 cell line

Supported Phospholipid Bilayer Interaction with Components Found in Typical Room-Temperature Ionic Liquids – a QCM-D and AFM Study

The RNA of the human syncytium-forming (foamy) virus

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