A Rapid, High Frequency Regeneration of &lt;i&gt;Justicia gendarussa&lt;/i&gt; Burm.f.

Janarthanam, B.; Gayathri, B; Sumath, E

doi:10.3329/bjsir.v46i2.4388

Cited by 5 publications

(5 citation statements)

References 5 publications

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“…Similarly, Reddy et al [ 28 ] reported similar results for Ceropegia ensifolia . The addition of 30 g/L sucrose was found to be the optimum for the growth of Thymus hyemalis compared to other types of carbon sources including glucose, fructose, mannitol and sorbitol [ 17 ]. The studies implied that a sucrose concentration higher than 30 g/L results in a decrease in the multiplication and growth rate of plants [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, 12 µM BAP was more favorable than other cytokinins in the initial stage of the shoot induction of C. nutans. A study on Justicia gendarussa (Acanthaceae) showed that the supplementation of BAP in an MS basal medium exhibited the highest percentage of shoot induction, number of shoots and shoot length, compared to a basal medium supplemented with kinetin and thidiazuron (TDZ) [17,18]. In addition, similar studies on Andrographis paniculate, which belong to the same family as C. nutans and J. gendarussa, also recorded that the application of BAP was able to produce a higher percentage of shoot regeneration and number of shoots [19].…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)

Haida

Nakasha

Hakiman

2020

Plants

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Clinacanthus nutans, commonly known as Sabah snake grass, is one of the more important medicinal plants in Malaysia’s herbal industry. C. nutans has gained the attention of medical practitioners due to its wide range of bioactive compounds responsible for various biological activities, such as anti-cancer, anti-venom and anti-viral activities. Due to its high pharmacological properties, the species has been overexploited to meet the demands of the pharmaceutical industry. The present study was conducted to establish a suitable in vitro culture procedure for the mass propagation of C. nutans. Murashige and Skoog (MS) basal medium, supplemented with different types of cytokinins, auxins, basal medium strength and sucrose concentrations, were tested. Based on the results, a full-strength MS basal medium supplemented with 12 µM 6-benzylaminopurine (BAP) and 30 g/L sucrose was recorded as the best outcome for all the parameters measured including the regeneration percentage, number of shoots, length of shoots, number of leaves and fresh weight of leaves. In the analysis of the phenolics content and antioxidant activities, tissue-cultured leaf extracts assayed at 100 °C exhibited the highest phenolic content and antioxidant activities. The propagation of C. nutans via a plant tissue culture technique was recorded to be able to produce high phenolic contents as well as exhibit high antioxidant activities.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)

Haida

Nakasha

Hakiman

2020

Plants

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“…On the contrary, previous research on Justicia gendarussa found that supplementing BAP in MS basal medium resulted in the highest percentage of shoot induction, the number of shoots, and shoot length compared to KIN and thidiazuron (TDZ) (Janarthanam et al, 2011). The same finding was observed in Clinacanthus nutans, in which 12 μM BAP was more effective than the other cytokinins in the early stages of shoot induction (Haida et al, 2020).…”

Section: Shoot Multiplication Of M Pudica In Response To Different Pl...mentioning

confidence: 78%

Revisiting In vitro Micropropagation Protocols of Mimosa pudica for Enhanced Seed Germination, Shoot Multiplication, and Root Initiation

Baharuddin¹,

Roslan²,

Ramli³

et al. 2023

JTAS

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Mimosa pudica is a medicinal plant worthy of therapeutic properties. It is often overlooked as one of the weed species, and the potential was underappreciated despite its abundance in nature, particularly in tropical climate countries. Considering the aptitude of this species, the micropropagation protocol of M. pudica was revisited and enhanced. The seed surface sterilization and germination were assessed, followed by shoot multiplication rate and root initiation efficiency. Seeds of M. pudica were best surface sterilized with 35% of Clorox and recorded the highest germination rate at 65.55% in media of three-quarter strength Murashige and Skoog (MS) combined with 30 g of sucrose. The explants were harvested and subcultured into multiplication media fortified with 6-benzylaminopurine, kinetin, and coconut water. The highest number of new shoots and leaves were obtained at 3 shoots and 3.27 cm in media of MS combined with 17.6 μm kinetin. Half-strength MS media devoid of hormone was recommended for root induction, resulting in the 5 healthy roots at 2.06 cm after 4 weeks of culture. Overall, the developed micropropagation protocol of M. pudica could facilitate its large-scale cultivation, indicating its potential as a medicinal crop for the extraction of bioactive compounds.

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“…At lower and higher concentrations of BAP (1.11 and 8.88 µM), TDZ (1.14 and 9.08 µM) and Kn (1.16 and 9.20 μM) did not significantly induce the number of shoots per explant. BAP has been considered to be one of the most active cytokinins for organogenic differentiation in plant tissue culture (Janarthanam et al 2009and 2011, Biswas et al 2009, Sudharson et al 2014. Nodal explants on MS without growth regulator did not initiate shoot differentiation.…”

Section: Resultsmentioning

confidence: 99%

In vitro Plant Regeneration from Nodal Explants of Coleus forskohlii Briq. - An Important Medicinal Plant

Janarthanam¹,

Sumathi

2020

Plant Tissue Cult. & Biotech.

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An efficient in vitro mass propagation and promising protocol has been successfully standardized and developed for Coleus forskohlii through direct organogenesis from nodal explants. Nodal explants cultured onto MS basal medium supplemented with 4.44 μM BAP recorded the highest response and produced 24.3 ± 0.2 shoots per explant with an average shoot length 5.6 ± 0.4 cm after 30 days of culture. The in vitro shoots recorded higher response for development of rooting on half strength MS fortified with 2.46 μM IBA which produced the best response 7.8 ± 0.6 roots per in vitro shoot with an average root length of 4.3 ± 0.1 cm after 25 days. The in vitro rooted plantlets were transferred for hardening and 90% of the plantlets survived were successfully acclimatized and established in small plastic pots. This protocol recorded to be a highly repeatable, successful and rapid technique that could be utilized for the commercial mass propagation and ex situ conservation of Coleus forskohlii. It is important to note that the morphology of the in vitro plantlets of Coleus forskohlii showed a true-to-type growth habit, both in vitro and when transferred to ex vitro growth conditions. Plant Tissue Cult. & Biotech. 30(1): 143-148, 2020 (June)

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A Rapid, High Frequency Regeneration of <i>Justicia gendarussa</i> Burm.f.

Cited by 5 publications

References 5 publications

In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)

In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)

Revisiting In vitro Micropropagation Protocols of Mimosa pudica for Enhanced Seed Germination, Shoot Multiplication, and Root Initiation

In vitro Plant Regeneration from Nodal Explants of Coleus forskohlii Briq. - An Important Medicinal Plant

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