2014
DOI: 10.1016/j.foodchem.2014.02.150
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A rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk

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Cited by 27 publications
(16 citation statements)
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“…Development of anti-casein-capture probe labelled with GMNP GMNP (3 mg) by the solvothermal method (Song et al, 2014) was washed three times with 2 mL PBS buffer (0.01 mol/L, pH7.4) and then 500 μL McAb (5 μg/mL) was added to synthesize capture probe (in Figure 1(A)) by electrostatic adsorption. The mixture was incubated at 37°C for 60 min.…”
Section: Development Of Anti-casein-detection Probe Labelled With Cdtmentioning
confidence: 99%
See 1 more Smart Citation
“…Development of anti-casein-capture probe labelled with GMNP GMNP (3 mg) by the solvothermal method (Song et al, 2014) was washed three times with 2 mL PBS buffer (0.01 mol/L, pH7.4) and then 500 μL McAb (5 μg/mL) was added to synthesize capture probe (in Figure 1(A)) by electrostatic adsorption. The mixture was incubated at 37°C for 60 min.…”
Section: Development Of Anti-casein-detection Probe Labelled With Cdtmentioning
confidence: 99%
“…Data were the means of triplicates. Detection limit (LOD) of the assay was calculated as the mean value of blanks plus three times standard deviation of the mean (Song et al, 2014).…”
Section: Development and Characterization Of The Dual-labelled Immunomentioning
confidence: 99%
“…Coupling principle (Song et al, 2014) was illustrated as follows (Figure 1(A)). A large amount of gold nanoparticles were connected with on Fe 3 O 4 /Au nanoparticle composites surface.…”
Section: Conjugation Proceduresmentioning
confidence: 99%
“…Magnetic bead based on immunoassay was first established in the 1980s because of the main advantages of increasing the surface area for immobilization of antibody or antigen, reducing the incubation time from hours to minutes, and improving sensitivity of the assay (Biagini et al, 2004;Moscoso, Kiefer, Kutlar, & Garver, 1988). Magnetic bead-based immunoassay was comprehensively applied in detection of macromolecular substances, such as β-bungarotoxin (Mu et al, 2014), β-casein allergen (Li et al, 2015;Song et al, 2014), enterobacter cloacae (Zhang, Zhou, Zhang, Zhang, & Su, 2016), pathogens, and cancer biomarkers (Baniukevic et al, 2013;Chen et al, 2016;Shukla, Lee, Song, Park, & Kim, 2016). However, the application of goldmag-based immunoassay in the quantification of α-LA content in milk has not been studied.…”
Section: Introductionmentioning
confidence: 99%
“…In order to prepare IMBs, hydrophilic magnetic beads with carboxyl groups on the surface were used because carboxyl acid groups on the surface of MBs could be coupled with the amino groups in MAbs by EDC and NHS (Jang & Keng, 2008;Song et al, 2014). To prepare IMBs, several crucial components and parameters were optimized.…”
Section: Preparation Of Imbsmentioning
confidence: 99%