A rapid method to assess the aggressiveness of Eutypa lata isolates and the susceptibility of grapevine cultivars to Eutypa dieback

Péros, J. P.; Berger, Gilles

doi:10.1051/agro:19940804

Cited by 43 publications

(42 citation statements)

References 6 publications

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“…Interestingly, only eutypa dieback symptoms have been reproduced using artificial infection (Petzoldt et al 1981;Péros and Berger 1994;Sosnowski et al 2007;Camps et al 2010). In contrast, no studies have reported the artificial reproduction of foliar symptoms caused by esca or botryosphaeria dieback on grapevine.…”

Section: Introductionmentioning

confidence: 99%

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

et al. 2014

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Three major grapevine trunk diseases, esca, botryosphaeria dieback and eutypa dieback, pose important economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. The different fungi associated with grapevine trunk diseases can be isolated in the necrotic wood, but not in the symptomatic leaves. Other factors seem to be responsible for the foliar symptoms and may represent the link between wood and foliar symptoms. One hypothesis is that the extracellular compounds produced by the fungi associated with grapevine trunk diseases are responsible for pathogenicity.In the present work, we used Vitis vinifera cv. Chardonnay cells to test the aggressiveness of total extracellular compounds produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with botryosphaeria dieback. Additionally, the toxicity of purified mellein, a characteristic toxin present in the extracellular compounds of Botryosphaeriaceae, was assessed.Our results show that the total extracellular compounds produced by N. parvum induce more necrosis on Chardonnay calli and induce a different defence gene expression pattern than those of D. seriata. Mellein was produced by both fungi in amounts proportional to its aggressiveness. However, when purified mellein was added to the culture medium of calli, only a delayed necrosis and a lower-level expression of defence genes were observed. Extracellular compounds seem to be involved in the pathogenicity of the fungi associated with botryosphaeria dieback. However, the doses of mellein used in this study are 100 times higher than those found in the liquid fungal cultures: therefore, the possible function of this toxin is discussed.

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Section: Introductionmentioning

confidence: 99%

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

et al. 2014

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“…Differences in disease severity among plantations are more likely related to management practices, i.e., pruning intensity or strategy, than to cultivar resistance or tolerance. In grapevine cultivars differences have been found in their susceptibility to E. lata and subsequent symptom development (Peros and Berger 1994). However, no grape cultivar is known to be immune to infection.…”

Section: Discussionmentioning

confidence: 99%

Eutypa lata, the causal agent of dieback in red currant (Ribes rubrum) and gooseberry (R. uva-crispa) in the Netherlands

Wenneker

Raak²,

Brouwershaven³

et al. 2011

Eur J Plant Pathol

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Dieback of red currant (Ribes rubrum) and gooseberry (Ribes uva-crispa) is an increasing problem in commercial fields in the Netherlands. Field surveys were done in 2006-2007 and samples with dieback symptoms were analysed. In this study the causal agent was diagnosed as Eutypa lata, based on morphological characteristics and rDNA-ITS sequence data. The field surveys revealed the presence of the anamorph and teleomorph states of the fungus produced on dead infected currant wood. Eutypa lata is a vascular pathogen of many woody plants. Related fungi from the same family Diatrypaceae are difficult to distinguish from E. lata based on morphological features. The genetic variability of E. lata was compared by rDNA-ITS sequencing of isolates from different hosts and origins. Within the E. lata isolates little variability in the ITS sequences was observed. Phylogenetic analysis showed no clear subdivisions within the species. Eutypa lata strains isolated from the different hosts were closely related, indicating that there is no direct evidence for host specificity.

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“…We used 60 E. lata isolates that originated from different geographic locations and that were obtained from the wood or stromata of grapevines (Table 1). Isolates were stored long-term in distilled water at 4°C, as previously described (25). Some cultures were initiated by transferring mycelial plugs on malt-agar (MA) medium (15 g of Cristomalt [Materne, Fruibourg, France] per liter, 20 g of agar per liter) to petri dishes.…”

Section: Methodsmentioning

confidence: 99%

PCR Assays That Identify the Grapevine Dieback FungusEutypa lata

Lecomte

Péros²,

Blancard

et al. 2000

Appl Environ Microbiol

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Eutypa lata is the causal fungal agent of Eutypa dieback, a serious grapevine necrotic disease. The erratic and delayed (1 to 2 months) appearance of characteristic conidia on culture media and the presence of numerous microorganisms in decaying wood make it difficult either to identify or to detect E. lata in grapevine wood samples. We designed six pairs of PCR primers for diagnosis of E. lata. Three primer pairs were derived from ribosomal DNA internal transcribed spacer sequences, and three pairs were derived from randomly amplified polymorphic DNA fragments. The six primer pairs could be used to amplify DNAs extracted from all of the E. lata isolates tested. They did not amplify DNAs from fungi and bacteria representing more than 50 different species of microorganisms associated with grapevine. We developed a simple protocol, leading to a rapid release of DNA, that enabled us to identify E. lata from pure or mixed cultures as well as from grapevine wood samples. Identification of E. lata in wood was achieved within a few hours, instead of the several weeks required for classical cultures on agar medium. We believe that the procedure described here can be adapted to detect other microorganisms involved in woody plant diseases.Eutypa lata (Pers.: Fr.) Tul et C. Tul. (synonym E. armeniacae Hansf. and M. V. Carter; anamorph Libertella blepharis A. L.

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A rapid method to assess the aggressiveness of Eutypa lata isolates and the susceptibility of grapevine cultivars to Eutypa dieback

Cited by 43 publications

References 6 publications

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

Eutypa lata, the causal agent of dieback in red currant (Ribes rubrum) and gooseberry (R. uva-crispa) in the Netherlands

PCR Assays That Identify the Grapevine Dieback FungusEutypa lata

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