A Rapid Separation and Highly Determination of Paraben Species by Ultra-Performance Liquid Chromatography —Electrochemical Detection

Abstract

In this study, a new technique was developed using rapid ultra-performance liquid chromatography (UPLC)-based separation coupled with electrochemical detection by a boron-doped diamond (BDD) electrode for the detection and quantification of three commonly used parabens (methylparaben (MP), ethylparaben (EP) and propylparaben (PP)). We aimed to reduce the analysis time by using UPLC coupled with a short reverse phase C 18 monolithic column (25 mm × 4.6 mm). Operating the monolithic column at low back-pressure resulted in high flow rates. A mobile phaseconsisting of a 25:75 (v/v) ratio of acetonitrile: 0.05 M phosphate buffer (pH 5) at a flow rate of 2.5 mL•min −1 was used to perform the separation. The amperometric detection with the BDD electrode was found to be optimal and reliably reproducible at a detection potential of 1.5 V vs. Ag/AgCl. Under these conditions, the separation of the three targetanalytes (MP, EP and PP) was achieved in 2 min and was linear within a sample concentration range of 0.1 to 50.0 mg•L −1 (r 2 values of 0.9970, 0.9994 and 0.9994 for MP, EP and PP, respectively). This method was successfully applied to determine the concentrations of each parabeninsix real samples with therecoveries ranging from of 80.3%-98.9% for all three parabensfrom samples spiked at 12, 22 and 32 mg•L −1. Therefore, the proposed method can be used as an alternative rapid and selective method for the determination of paraben levels in real samples.

Ultrasound-assisted cloud point microextraction of certain preservatives in real samples and determination by HPLC

“…9,10 For these reasons, it is important to develop a simple and reliable method for the identication of parabens in various samples. High performance liquid chromatography (HPLC), 11 liquid chromatography (LC), 12 LC-electrochemical and ultra-violet (UV) detection, 13 gas chromatography-mass spectroscopy (GC-MS), 14 liquid chromatography-mass spectroscopy (LC-MS) 15 and capillary electrophoresis 16 have oen been used for the determination of various paraben derivatives. There are also electroanalytical 17 and spectroscopy methods.…”

Paper spray tandem mass spectrometry: A rapid approach for the assay of parabens in cosmetics and drugs

“…Several methods for the assessment of parabens in different matrices, including cosmetics and pharmaceuticals, have been published. Conventionally, they are analyzed by gas chromatography (GC) or high‐performance liquid chromatography (HPLC) coupled with mass spectrometry (MS), ultraviolet, and electrochemical detection 17–23 . MS is certainly the most appropriate tool for a specific and sensitive analysis 24,25 ; moreover, this technique allows the use of labeled internal standards, which assure a high grade of accuracy in the analysis 26,27 .…”