A Rapid Serologic Test and Immunoblotting for the Detection of Helicobacter pylori Infection in Children

Treepongkaruna, Suporn; Nopchinda, Supujchara; Taweewongsounton, Aruchalean; Atisook, Kanit; Pienvichit, Pavit; Vithayasai, Niyada; Simakachorn, Nipat; Aanpreung, Prapun

doi:10.1093/tropej/fmk003

Cited by 16 publications

(18 citation statements)

References 26 publications

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“…Performance of ICT with CIM evaluated in this study was better than two other commercial ICT kits evaluated in Chinese patients [30] and similar to another commercial ICT kit evaluated in African-American, Caucasian, and Asian population with 13 C urea breath test as the 'gold standard' [31] . Its performance in our study was lower than a study that evaluated this kit in Thai children [19] and similar to a study that evaluated this kit in Portuguese children [20] . Performance of immunoblot with CIM in our study was similar to reports that evaluated the same kit in adult French population [16] and Portuguese children of different age groups [17] .…”

Section: Discussioncontrasting

confidence: 58%

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Non-invasive diagnosis of H pylori infection: Evaluation of serological tests with and without current infection marker CIM

Rahman¹,

Azam²,

Rahman³

et al. 2008

WJG

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Section: Discussioncontrasting

confidence: 58%

“…Performance of serological tests depends on the antigen preparation used [12,14] , and as H pylori strains differ among geographic locations, local validation of the test is necessary [13,14] . [15][16][17][18][19][20] . Bangladesh is a developing country with a high prevalence of H pylori infection and peptic ulcer [21,22] .…”

Section: Wwwwjgnetcommentioning

confidence: 99%

Non-invasive diagnosis of H pylori infection: Evaluation of serological tests with and without current infection marker CIM

Rahman¹,

Azam²,

Rahman³

et al. 2008

WJG

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“…The advantages of H. pylori multiplex western blotting assays include their capacity to simultaneously identify antigen-specific antibody responses which can replace biopsy-based methods for virulence profiling of the infecting H. pylori strain. Hence, multiplex western blotting becomes a particularly attractive method for application in children (Ögünç et al, 2003;Treepongkaruna et al, 2006) and the elderly (Simán et al, 2005) population for whom endoscopy-based methods are not advised.…”

Section: Discussionmentioning

confidence: 99%

Seroreactivity to Helicobacter pylori Antigens as a Risk Indicator of Gastric Cancer

Karami¹,

Talebkhan²,

Saberi³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Multiple etiologic factors are suspected to cause gastric cancer, the most important of which is infection with virulent types of Helicobacter pylori. Materials and Methods: We have compared 102 gastric cancer patients with 122 non-ulcer, non-cancer dyspeptic patients. Gastric specimens were evaluated for H. pylori infection by tissue-based detection methods. Patient sera underwent antigen-specific ELISA and western blotting using a Helicoblot 2.1 kit and antibody responses to various H. pylori antigens were assessed. Results: The absolute majority (97-100%) of both groups were H. pylori seropositive. Multivariate regression analysis demonstrated serum antibodies to the low molecular weight 35kDa protein to be protective and reduce the risk of gastric cancer by 60% (OR:0.4; 95%CI:0.1-0.9). Conversely, seroreactivity to the 89kDa (VacA) protein was significantly higher in gastric cancer patients (OR:2.7; 95%CI:1.0-7.1). There was a highly significant association (p<0.001) between seroreactivity to the 116kDa (CagA) and 89kDa (VacA) proteins, and double positive subjects were found at nearly five fold (OR:4.9; 95%CI:1.0-24.4) enhanced risk of gastric cancer as compared to double negative subjects. Conclusions: Seroreactivity to H. pylori low (35kDa) and high (116kDa/89kDa) molecular weight antigens were respectively revealed as protective and risk indicators for gastric cancer.

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“…Two articles measured IgG against H. pylori using a rapid immunochromatography test in whole blood. They showed sensitivity as low as 54% with better specificity [93,115].…”

Section: Serologymentioning

confidence: 99%

“…Four studies used immunoblots to measure IgG against H. pylori [87,90,97,115]. All measured IgG and showed sensitivity and specificity above 79%.…”

Section: Serologymentioning

confidence: 99%

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

et al. 2009

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The array of tests that can be used for diagnosis of Helicobacter pylori infection is large, and it can be confusing to define which test to use particularly in children where results may not be comparable to those obtained in adult patients. Using PubMed, we reviewed the English literature from January 1999 to May 2009 to identify articles that determined sensitivity and specificity of H. pylori invasive and non-invasive diagnostic tests in children. We excluded articles that presented a review of the literature, abstracts, case reports, or series where children's results could not be separated from adult populations. Of the tissue based methods, rapid urease tests have better sensitivity than histology to detect presence of H. pylori; however, histology can detect the pathology associated with disease including gastritis, intestinal metaplasia, and other conditions that could be the cause of the child's symptoms. Culture of gastric tissues or stool has 100% specificity but sensitivity is low. Of the serologic tests, immunoblot has the best sensitivity. The urea breath tests have >75% sensitivity for detection of H. pylori before and after treatment. Immunoassays in stool using monoclonal antibodies have >95% sensitivity for detection of H. pylori before and after treatment. PCR testing can be performed in tissue and stool samples and can detect genes associated to antibiotic resistance. In summary, the current commercial non-invasive tests have adequate sensitivity and specificity for detecting the presence of H. pylori; however, endoscopy with histopathology is the only method that can detect H. pylori and lesions associated with the infection.

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A Rapid Serologic Test and Immunoblotting for the Detection of Helicobacter pylori Infection in Children

Cited by 16 publications

References 26 publications

Non-invasive diagnosis of H pylori infection: Evaluation of serological tests with and without current infection marker CIM

Non-invasive diagnosis of H pylori infection: Evaluation of serological tests with and without current infection marker CIM

Seroreactivity to Helicobacter pylori Antigens as a Risk Indicator of Gastric Cancer

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

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