A Rapid, Simple Spectrophotometric Method for Simultaneous Detection of Nitrate and Nitrite

Miranda, Katrina M.; Espey, Michael Graham; Wink, David A.

doi:10.1006/niox.2000.0319

Cited by 3,224 publications

(1,794 citation statements)

References 49 publications

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“…NO 2 − concentrations were determined by the sulfanilamide coloration method [28]. NO 3 − concentrations were determined after vanadium reduction to nitrite and quantified in this form following the nitrite determination procedure [28,29]. SO 4 2− concentrations were measured using ion chromatography.…”

Section: Methodsmentioning

confidence: 99%

Vertical Distribution of Functional Potential and Active Microbial Communities in Meromictic Lake Kivu

et al. 2015

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The microbial community composition in meromictic Lake Kivu, with one of the largest CH 4 reservoirs, was studied using 16S rDNA and ribosomal RNA (rRNA) pyrosequencing during the dry and rainy seasons. Highly abundant taxa were shared in a high percentage between bulk (DNA-based) and active (RNA-based) bacterial communities, whereas a high proportion of rare species was detected only in either an active or bulk community, indicating the existence of a potentially active rare biosphere and the possible underestimation of diversity detected when using only one nucleic acid pool. Most taxa identified as generalists were abundant, and those identified as specialists were more likely to be rare in the bulk community. The overall number of environmental parameters that could explain the variation was higher for abundant taxa in comparison to rare taxa. Clustering analysis based on operational taxonomic units (OTUs at 0.03 cutoff) level revealed significant and systematic microbial community composition shifts with depth. In the oxic zone, Actinobacteria were found highly dominant in the bulk community but not in the metabolically active community. In the oxic-anoxic transition zone, highly abundant potentially active Nitrospira and Methylococcales were observed. The cooccurrence of potentially active sulfur-oxidizing and sulfatereducing bacteria in the anoxic zone may suggest the presence of an active yet cryptic sulfur cycle.

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Section: Methodsmentioning

confidence: 99%

Vertical Distribution of Functional Potential and Active Microbial Communities in Meromictic Lake Kivu

et al. 2015

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“…Colonic NO level was estimated as nitrite and nitrate by the acidic Griess reaction after reduction of nitrate to nitrite by vanadium trichloride according to the method described by Miranda et al [18] . The Griess reaction relies on a simple colorimetric reaction between nitrite, sulfonamide and N-(1-naphthyl) ethylenediamine to produce a pink azo-product with maximum absorbance at 543 nm.…”

Section: Determination Of Colonic Nitrite/nitrate Levelmentioning

confidence: 99%

Effect of hydroalcoholic extract of Hibiscus rosa sinensis Linn. leaves in experimental colitis in rats

Kandhare

Raygude

Ghosh

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

100

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“…The concentrations of nitrate and nitrite were detected by the methods of Miranda et al [24]. Nitrite and nitrate calibration standards were prepared by diluting sodium nitrite and sodium nitrate in pure water.…”

Section: Nitrate and Nitrite Analysesmentioning

confidence: 99%

“…Nitrite was measured in a similar manner, except that samples and nitrite standards were only exposed to Griess reagents. The absorbance at 540 nm was read to assess the total plasma level of nitrite and nitrate in all samples [24].…”

Section: Nitrate and Nitrite Analysesmentioning

confidence: 99%

Does dexmedetomidine reduce secondary damage after spinal cord injury? An experimental study

et al. 2009

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The aim of this experimental study was to investigate the possible protective effect of dexmedetomidine (DEX) on traumatic spinal cord injury (SCI). Twentytwo New Zealand rabbits were divided into three groups: sham (no drug or operation, n = 6), Control [SCI ? single dose of 1 mL saline intraperitoneally (i.p), after trauma; n = 8] and DEX (SCI ? 1 lg/kg dexmedetomidine in 1 mL, i.p, after trauma, n = 8). Laminectomy was performed at T10 and balloon angioplasty catheter was applied extradurally. Four and 24 h after surgery, rabbits were evaluated by an independent observer according to the Tarlov scoring system. Blood, cerebrospinal fluid (CSF), tissue samples from spinal cord were taken for biochemical and histopathological evaluations. After 4 h of SCI, all animals in control or DEX treated groups became paraparesic. On the other hand, 24 h after SCI, partial improvements were observed in both control and DEX treated groups. Traumatic SCI leads to increase in the lipid peroxidation and decreases enzymatic or nonenzymatic endogenous antioxidative defense systems. Again, SCI leads to apoptosis in spinal cord. DEX treatment slightly prevented lipid peroxidation and augmented endogenous antioxidative defense systems in CSF or spinal cord tissue, but failed to prevent apoptosis or neurodeficit after traumatic SCI. Therefore, it could be suggested that treatment with dexmedetomidine does not produce beneficial results in SCI.

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A Rapid, Simple Spectrophotometric Method for Simultaneous Detection of Nitrate and Nitrite

Cited by 3,224 publications

References 49 publications

Vertical Distribution of Functional Potential and Active Microbial Communities in Meromictic Lake Kivu

Vertical Distribution of Functional Potential and Active Microbial Communities in Meromictic Lake Kivu

Effect of hydroalcoholic extract of Hibiscus rosa sinensis Linn. leaves in experimental colitis in rats

Does dexmedetomidine reduce secondary damage after spinal cord injury? An experimental study

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