A rapid, targeted, neuron-selective, in vivo knockdown following a single intracerebroventricular injection of a novel chemically modified siRNA in the adult rat brain

Nuclear-translocated Glyceraldehyde-3-phosphate Dehydrogenase Promotes Poly(ADP-ribose) Polymerase-1 Activation during Oxidative/Nitrosative Stress in Stroke

“…siRNA to control, e.g. non-targeting siRNA, (5Ј-UGGUU- UACAUGUCGACUAA-3Ј) or rat GAPDH (rGAPDH; 5Ј-UCU-ACAUGUUCCAGUAUGA-3Ј, Accell siRNA from Dharmacon) was intracerebroventricularly introduced according to a previously published method (26). The AAV2-injected rats were placed in a stereotaxic instrument (Narishige).…”

Short interfering RNA and the central nervous system: development of nonviral delivery systems

“…To increase the stability and efficiency of siRNA, several chemical modifications ('Accell siRNA') have been introduced into siRNA nucleotides. Although its mechanism of delivery is unknown, chemically modified siRNA reduced expression of target genes in neurons, but not glia, after ICV administration [12]. These results suggest that chemical modification is necessary to deliver siRNA to the CNS even when using ICV administration.…”

“…Gene silencing using ICV administration of siRNA has been reported [10][11][12]. The ICV infusion of naked siRNA into the third ventricle of the mouse brain suppressed a target gene at a dose of 3 µmol [10], but another study showed that naked siRNA does not reach brain cells as well as naked antisense oligonucleotide after ICV administration [11].…”

Silencing of Id2 attenuates hypoxia/ischemia-induced neuronal injury via inhibition of neuronal apoptosis

“…A 28-gauge stainless steel injection cannula was paved into the right lateral ventricle as previously described [25]. Then the rats received a single injection of Id2-siRNA or misRNA or 0.9% NaCl at a rate of 0.5µl/min using a micorinfusion pump and a 10 µl microsyringe.…”