A Rapid Test Strip for Diagnosing Glycosylated Hemoglobin (HbA1c) Based on Fluorescent Affinity Immunochromatography

In this paper, gold nanoparticles were combined with aflatoxin B1-aptamer as a single probe via the specific binding between aflatoxinB1 with aptamer, designing a colorimetric detection method. Meanwhile, a chromatographic strip with gold nanoparticles modified by complementary chains of aflatoxin B1 aptamer as the probe was developed for rapid detection of aflatoxin B1. Under the optimal conditions, the linear detection range of the colorimetric method was 0.5–50 ng/mL, the minimum detection limit was 0.5 ng/mL, the detection range of the chromatographic strip was 0.5–500 ng/mL, the minimum detection limit was 0.5 ng/ mL, and the semi-quantitative limit of detection was 51 ng/ml. Finally, comparing the two methods, it is concluded that chromatographic strip detection is more sensitive, accurate and celerity. Sample recovery rate of the latter was from 97.3–120%. All detections could be accomplished within 10 min, indicating that the test strip could be a potentially effective tool for rapid on-site detections.

show abstract

A Rapid Test Strip for Diagnosing Glycosylated Hemoglobin (HbA1c) Based on Fluorescent Affinity Immunochromatography

Cited by 7 publications

References 29 publications

Fluorescence/electrochemiluminescence approach for instant detection of glycated hemoglobin index

Fluorescence/electrochemiluminescence approach for instant detection of glycated hemoglobin index

Comparability of different methods of glycated hemoglobin measurement for samples of patients with variant and non-variant hemoglobin

Aptamer-based Colorimetric and Lateral Flow Chromatographic Strip detection of Aflatoxin B 1 in corn Samples

Contact Info

Product

Resources

About