A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity

Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline Phosphatases

“…From these results, the authors speculated that the bright emission in the yellow channel is attributable to ALP-catalyzed dephosphorylation of the probe. [95]. Its molecular engineering associated it with the phosphatase-regulated ESIPT phenomenon (Figure 61).…”

“…ALP-29 showed excellent selectivity towards ALP without showing any interference from the other enzymes, such as acetylcholinesterase, trypsin or lysozyme. The MTT-based cytotoxicity assay in HeLa cell lines revealed that [95]. Its molecular engineering associated it with the phosphatase-regulated ESIPT phenomenon (Figure 61).…”

“…(b) FCIMs of HeLa cells pretreated with NaH 2 PO 4 or levamisole and then incubated with ALP-28. Figures were adapted, modified and reproduced with copyright permission from Elsevier Ltd., 2019 [94].Yangyang et al reported a benzothiazole-based ratiometric probe, (4-benzamio-2-(benzo[d] thiazol-2-yl)phenyl dihydrogen phosphate (ALP-29), for the intracellular recognition of ALP[95]. Its molecular engineering associated it with the phosphatase-regulated ESIPT phenomenon (Figure61).…”

Highly Sensitive Alkaline Phosphatase Biosensor Based on Internal Filtration Effect between G-Quadruplex/N-methylmesoporphyrin IX and p-Nitrophenol

“…Among them, fluorescence methods have attracted great attention due to their high sensitivity. Some fluorescence materials, such as nanoclusters 14,15 , organic fluorophores 16,17 , quantum dots [18][19][20] and carbon dots 21,22 , have been successfully used to monitor ALP. However, most of these methods were subject to some limitations, such as expensive reagents, long synthesis time and complex synthesis routes.…”

Sensitive sensing of alkaline phosphatase and γ-glutamyltranspeptidase activity for tumor imaging

“…Alkaline phosphatase (ALP) and γ-glutamyltranspeptidase (GGT) are two important biomarkers for many pathological processes, 22,23 including cancers. It has been reported that ALP is overexpressed in osteoblastic bone cancer, 24 liver cancer, 25 breast cancer, 26 and prostatic cancer, 26 and the malignant tumors occurring in the liver, 27 cervix, 22 ovary, 28 breast 27 and pancreas 29 were found with abnormally higher levels of GGT. BLI probes were reported to individually sense ALP 30 or GGT 31 activity, but none can detect both ALP and GGT activity using a single molecular probe, which may not only boost the response rate of the probe but also promote the sensitivity for tumor imaging.…”