A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis

Chen, Jing; Zhang, Lida; Paoli, George C.; Shi, Chunlei; Tu, Shu‐I; Shi, Xianming

doi:10.1016/j.ijfoodmicro.2009.12.004

Cited by 110 publications

(71 citation statements)

References 29 publications

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“…The apparent prevalence is higher than the study conducted in Pakistan (35%) [30] , India [17] (28%). This variation may be causes due to small sampling strategy of our study or sensitivity of diagnosing tools used for the detection of salmonella [31] . Alternatively, it could be higher for the farm level prevalence of salmonellosis in birds.…”

Section: Discussionmentioning

confidence: 97%

Determination of Salmonella in egg shell and egg content in some selected areas of Bangladesh

et al. 2016

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Salmonellosis is one of the most common and widely distributed food-borne diseases. It constitutes a major public health burden and represents a significant cost in many countries. Salmonella are known for its wide range host and can cause clinical diseases in some hosts while in others, can be asymptomatic. Poultry and eggs are considered as major sources for different pathogenic Salmonella serotypes. Eggs produced locally under the small scale layer farm may present a hazard to consumers which may increase the spread of Salmonella in the environment. To investigate the occurrence of Salmonella, a total of 72 samples were taken from 6 poultry farm in some selected areas of Bangladesh. Sampling program was executed between October and December, 2013 and samples were tested using standard laboratory methods. Data was collected through direct interview and structured questionnaire. Our study shows that, true prevalence of Salmonella in egg shell and egg contents were 0.093% and 0.068% respectively. The highest apparent prevalence in Udayan poultry farm (50%) and lowest in Liza poultry farm (16.67%). The average frequency of egg shell contamination is positively correlated with re-use of egg tray without disinfection. To the best of our knowledge, this is the first investigation on Salmonella spp. in selected local egg production farms in Chittagong. Further detail study is highly recommended.

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Section: Discussionmentioning

confidence: 97%

Determination of Salmonella in egg shell and egg content in some selected areas of Bangladesh

et al. 2016

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“…One of these studies was a realtime PCR developed by Chen et al (5) was used for detecting SE in artificially contaminated foods, including liquid eggs. In another survey study by Gole et al (12) PCR was utilized to detect the presence of several virulence genes in Salmonella they isolated from eggs of commercial laying flocks.…”

Section: Discussionmentioning

confidence: 99%

“…International Organization for Standardization Method 6579 [ISO], United States Food and Drug Administration's Bacteriological Analytical Manual Chapter 5: Salmonella), which require up to 5 days (including biochemical and serological confirmations) (11,15), do not suffice in routine and rapid monitoring of these samples. In recent years, novel real-time PCR assays were developed for the rapid detection of Salmonella particularly from eggs (5). LightCycler PCR (LCPCR) is a specific real-time PCR system enabling rapid and reliable detection of the specific PCR product with probe-based technology and high sensitivity (3).…”

Section: Introductionmentioning

confidence: 99%

Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system

Temelli¹,

Kahya²,

Ata³

et al. 2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:This study aims to determine the presence of Salmonella in naturally contaminated grade A eggs by the standard culture method International Organization for Standardization Method 6579 (ISO) and a specific real-time PCR system (LightCycler PCR-LCPCR) to complement ISO. A total of 1635 eggs pooled into 101 samples were randomly collected within one year period from 20 different retail markets in Bursa, Turkey, carrying eggs of 16 large egg producers/suppliers of 5 cities with intensive layer production. Preparation of the egg and shell for analyses, Salmonella isolations and identifications, and detections were performed according to ISO 6887-4:2003, ISO 6579 and LCPCR, respectively. Overall Salmonella detection rate by ISO and LCPCR were 15.8 % (16/101) and 46.5 % (47/101), respectively. Out of 101 inner parts, Salmonella was detected in 11 (10.9 %) samples by ISO, and in 31 (30.7 %) samples by LCPCR. Six of 101 shell samples (5.9 %) were found to harbor Salmonella by ISO, while 18 (17.8 %) shells were positive by LCPCR. All isolates were determined as Salmonella enterica subsp. enterica serovar Enteritidis. These findings indicate considerably high Salmonella contamination in retail grade A eggs. This should be under routine monitoring by rapid methods such as PCR, complemented by standard culture to evaluate and assess the significance of risk for public health.

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“…diarrhea that is usually self-limiting to salmonellosis, mild fever, nausea, abdominal pains, enteric (typhoid) fever, bacteremia, septicemia and gastroenteritis in humans [35,36,37]. Among these, salmonellosis accounts for the major health, financial and socioeconomic burden caused by Salmonella species in infected patients worldwide [38,39,40,41].…”

Section: Journal Of Food and Nutrition Researchmentioning

confidence: 99%

Use of <i>invA</i> Gene Specific PCR Analysis for the Detection of Virulent <i>Salmonella</i> Species in Beef Products in the North West Province, South Africa

Ateba¹,

Mochaiwa²

2014

JFNR

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A total of 32 beef samples were collected from shops around the North West Province, South Africa and analyzed for the presence of Salmonella species. A total of 96 presumptive isolates were screened for characteristics of Salmonella. All (100%) of the isolates were Gram negative rods and catalase positive. However, only a small proportion (25%) utilized citrate as a sole carbon source while 78% of the isolates fermented the sugars glucose, lactose and sucrose. Gas was produced by 34.4% while only 3.1% of these isolates produced hydrogen sulphide gas. Despite the fact that a small proportion (12.5%) of these isolates were identified as Salmonella species by the API 20E test, a much larger proportion agglutinated with Salmonella Poly A-S antiserum (53.1%) when compared to the Salmonella Poly O antiserum. To avoid bias, all the 96 isolates were screened for the Salmonella specific invA gene through PCR analysis and only 10 (10.4%) isolates were positively identified. Moreover, none of the isolates possessed the fliC flagella gene while a small proportion 11(11.5%) were positive for the fljB gene fragments. The presence of these pathogenic Salmonella species in raw meat indicates the health risk that these food products can cause to consumers, especially if consumed undercooked.

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A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis

Cited by 110 publications

References 29 publications

Determination of Salmonella in egg shell and egg content in some selected areas of Bangladesh

Determination of Salmonella in egg shell and egg content in some selected areas of Bangladesh

Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system

Use of <i>invA</i> Gene Specific PCR Analysis for the Detection of Virulent <i>Salmonella</i> Species in Beef Products in the North West Province, South Africa

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